EMAGE mouse gene expression spatial database

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EMAGE:7710

Fbxl3 F-box and leucine-rich repeat protein 3 ( MGI:1354702)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007439_01	euxassay_007439_02	euxassay_007439_03	euxassay_007439_04

euxassay_007439_05	euxassay_007439_06	euxassay_007439_07	euxassay_007439_08	euxassay_007439_09

euxassay_007439_10	euxassay_007439_11	euxassay_007439_12	euxassay_007439_13	euxassay_007439_14

euxassay_007439_15	euxassay_007439_16	euxassay_007439_17	euxassay_007439_18	euxassay_007439_19

euxassay_007439_20	euxassay_007439_21	euxassay_007439_22	euxassay_007439_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7710_wholemount_strong.wlz

7710_wholemount_moderate.wlz

7710_wholemount_weak.wlz

7710_wholemount_possible.wlz

7710_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7710_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7828
Entity Detected:	Fbxl3, F-box and leucine-rich repeat protein 3 ( MGI:1354702)
Sequence:	sense strand is shown >T7828 CCGCGCGTGGCGGGGCAGCGAGGAGGACGGGCTCCTCCGGGCTCGCCCTGGGCGGCGCGGGTGCAGGCGG CTGGAAAGCCCGGGGGCCTGGGCGGTCAGTGTGTGCGGAAGCCGGGCCGGGGAGGCGCACGGTGGAAAAC TTGGTGAACTTAGGGGCAGCCGCCCGGGTCGCGTGCTGCAGACCACCACCCGGAAGCCGCCCGGGCAGGG CATCGCCGAGCCGCGTGCGAGGATGAAACGAGGAGGAAGAGATAGTGACCAGGACTCTGCTGAGGAAGGC ACTGCCGAGAAGCCCAAGAGACCGAGGACGACACAGGAGCGCTCGCAGCCTTGTGACTGGGGTAACCTCC TGCAGGACATCGTCCTCCATGTTTTTAAGTATTTGCCTCTTCTTGACCGGGCTCATGCTTCCCAAGTGTG CCGGAATTGGAATCAGGTATTTCACATGCCTGACTTGTGGCGATGTTTTGAATTTGAACTGAATCAGCCA GCGACATCTTACTTGAAAGCCACACACCCAGAGCTGATCAAACAGATTATCAAAAGGCACTCAAACCACC TACAGTATGTCAGCTTCAAGGTGGACAGCAGCAAAGAATCAGCTGAAGCTGCTTGTGACATACTGTCGCA GCTTGTGAATTGCTCTTTAAAAACTCTCGGACTTATCTCGACTGCCCGGCCAAGCTTTATGGATCTACCA AAGTCTCACTTTATCTCTGCACTGACAGTTGTGTTTGTAAACTCCAAGTCCCTGTCCTCACTTAAGATAG ACGACACCCCAGTCGATGACCCATCCCTTAAAGTCCTCGTGGCCAACAACAGCGACACACTCAAGCTGTT GAAAATGAGCAGCTGTCCTCACGTCTCTCCAGCAGGCATTCTGTGTGTGGCTGACCAGTGTCATGGCTTA CGAGAACTGGCCCTGAACTACCATTTGCTAAGTGATGAGTTGTTGCTCGCACTGTCTTCTGAAAAGCATG TTCGCTTAGAACATTTGCGCATTGATGTTGTCAGTGAGAACCCGGGACAGACACACTTCCACACTATCCA GAAGAGCAGCTGGGATGCCTTCATCAAACACTCACCGAAAGTCAACCTAGTGATGTATTTTTTTTTATAC GAAGAGGAATTTGACCCATTCTTTCGTTATGAAATACCTGCTACGCATCTTTACTTTGGGAGATCAGTCA GCAAAGATGTGCTTGGCCGTGTGGGCATGACCTGCCCGAGACTAGTGGAACTGGTAGTGTGTGCCAATGG GTTGCGGCCTCTTGATGAAGAGTTAATTCGCATTGCGGAACGTTGCAAAAATTTGTCAGCTATTGGGCTG GGGGAATGTGAAGTCTCATGTAGTGCCTTTGTTGAGTTTGTGAAGATGTGTGGGGGCCGCCTGTCTCAGT TATCCATTATGGAAGAAGTGTTAATTCCTGACCAAAAGTATAGTTTGGAACAAATTCATTGGGAAGTGTC TAAGCATCTTGGCAGGGTATGGTTCCCAGACATGATGCCTACTTGGTAAGGGACTGCATGACACAGGACA TAATGAGTGAGTAGTACCTTCATTCAAGAAACGTATTCTAACTAAAAGTTTATCTGCTCTAAAGCTGATA CAATGCTACTGTTCTGTGGCACAGAAATTTGATATCTTTGAGCTATGAATGATGTTTGTCAGAAGACTTA TAGTTTGGTTCAGATAACTCCTTTTTTGTAGCCTAATAGCAATCATATGTTTAAAAATTAAAAATGTGGT TTCATATTTGAAAGTAATATCCAATTATGAAATTGAGATAATTCAGTCTGAAGGAAGCAACCTGTATATT GTAATATCTCAGAAATGTACTAATAGGTTTTCTGAAATGTTCTCTATGCATTTTTTAAAAAAATGTAAAC TTGACATTTTAGGGTCTTCAGTTATAAATACACCTGTTATAAGGTGTTTAACATAGCTCAGGAAAGTGAG CATTTTGTGAGAGAAATGTATGATAGTGCTCGTAAGACCTTGGTTGAATGTTTTCAAACGTTATGAAGCT GTTTTAAAAATAAAGATATAACTATCAAAGTACTTAGAACGTTGATGAATGTTGAAGTGGCTGTTAAGGA GGGCACACAGAGCCAGGATCCAATCTGGAGAGATCCGTGGAGTAGGAGAGGTAGCACTCAGCCTTGTGTA ACTCTAGGTTTCTTGTGTGATATGCTAAACACATGCATTCTCTTTTGTTATATTACTAATGTCTGTGGTG CCTGAGGCATCGTGTGTAAAGGAATGCTTTGCTCTCTCTAAGTCTTTATTTAAACTATTAGTAATTATTT AAATTAAAATTTTACTTAAAAACCAAATTGAAATAATTAAAAGTAGAACTTTATAAATTGTTTATGAACA GTTTCATGAAAGGATATTAGTCTCCAGTGAACTTTAGTGATGGCTCATGCTTTAAGTTTGGAATTACATA GCTACAATAAATACAACTTTAAAAATCAAAGAGCATCTGTACAGTTTAGAAACACTAAATTTAAAAGAAG ATATATTTCATAATGATTTGCATAAATATTACTCAGTGAACTGGAACATTTTAATAGGAAAATATCTATA GTTTTGTGATTGTCAGTTTGGTTGAAACTGGTATTTTATATTAAGTTATGTAGCTTTTCATATTACTTTA ATATGAATAAAGGTAAATTCTAGTCTTTGTAGAATATAAAAATACTAAGTTTTGGTTATATTATAGTTTT GGGTTTTTGGTTTTCATTTTTTTAGATCCTGTATGAGCTTGGATTCTGGGCACTAGTGCTAACAGATGTC TCTCCTGAGAGAAGTTAGTAGTGACTTACCCCACAGGATGCTTCAGAGCCTGAAATGGCTCTTCAGTAGT AGTGGGACTTTTACAAAGTACCTTGATAATTATGGAAGCTAACAGTCTTAAAAATAGATAAACTTACTGA GACTAAAACAGAATGGCTGTCCACACAGTGCATGCTTTTCAAGAGTGGTGGTGTTCTTTCAGGCACGTAG GGATGAGAAGGAAAAGACCGACCCAGAGCTCCACACTAAAGGCAACTATTAGAATATTTCACTCTTAAAC AGTAAGAAGCTTTGACTGTACAGGAAATACTTGTGCTGTTTTGTGTTTTGTTTTTCTTTTTAATTTTTTT GAACAACATTGAAAAATGGAGTTGATGGTATATAGTCAGTTTTACTAACCAAAGCTTTATTTGAAAACTT TTGTACAAATTGAATTATATGCTTGAATACTTTAAGTCACTTTATAAGCAAAATGCATAGCACTTAATTT GTTTATACTGTAAAATATATGTTAAATGGTTTTATGAATTTGAGAATAAAGATAGTTACTAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6334317 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6334317 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7708 same embryo
	EMAGE:7707 same embryo
	EMAGE:7709 same embryo
	EMAGE:7706 same embryo
	EurExpress:euxassay_007439 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source