EMAGE mouse gene expression spatial database

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EMAGE:7712

Jph4 junctophilin 4 ( MGI:2443113)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007469. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007469_01	euxassay_007469_02	euxassay_007469_03	euxassay_007469_04

euxassay_007469_05	euxassay_007469_06	euxassay_007469_07	euxassay_007469_08	euxassay_007469_09

euxassay_007469_10	euxassay_007469_11	euxassay_007469_12	euxassay_007469_13	euxassay_007469_14

euxassay_007469_15	euxassay_007469_16	euxassay_007469_17	euxassay_007469_18	euxassay_007469_19

euxassay_007469_20	euxassay_007469_21	euxassay_007469_22	euxassay_007469_23	euxassay_007469_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7712_wholemount_strong.wlz

7712_wholemount_moderate.wlz

7712_wholemount_weak.wlz

7712_wholemount_possible.wlz

7712_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7712_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot mesenchyme

moderate

regional

moderate expression: see section 08 21 22

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 03 04 05

diencephalon

moderate

regional

moderate expression: see section 07 09 10 11 12 13 14 15 16 17 18

telencephalon

moderate

regional

moderate expression: see section 08

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24 moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon marginal layer

strong

regional

strong expression: see section 02 03 04 05 10 11 13 14 15 16 17 18 19 21 22 23 24 moderate expression: see section 06 07 08 09 12 20

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 14 15 16 17 18 moderate expression: see section 09 12

hindbrain

strong

regional

strong expression: see section 05 moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 22

midbrain

strong

regional

strong expression: see section 05 moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 18 19 20

trigeminal v nerve

moderate

regional

moderate expression: see section 10 17

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 17 weak expression: see section 10

cervical ganglion

weak

regional

weak expression: see section 10 18

thoracic ganglion

weak

regional

weak expression: see section 11 12

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8972
Entity Detected:	Jph4, junctophilin 4 ( MGI:2443113)
Sequence:	sense strand is shown >T8972 AGCCCCAGCACACATGCCACTCAGGATGAGGGTCCGGCCCTGCCTGCCCGCGCTGGGGCCCCTCCGCCCA GCCCCGGTCTAACTGCCCCCGCCCCCAAGCCTCGCCCGGCCCCCAGGCCCCCAGCCGGCTCTCCAGCCCC ATGATGCGCTGAGCTGCCGGGGGGCTGAGGCCGCGCCAACTACATGCATGTCCCCCGGGGGCAAGTTCGA CTTTGACGACGGGGGCTGCTACGTGGGGGGCTGGGAGGCGGGGCGGGCACATGGCTACGGCGTGTGCACC GGGCCCGGCGCCCAGGGAGAGTACAGCGGCTGCTGGGCTCACGGCTTCGAGTCGCTGGGTGTCTTCACGG GGCCCGGCGGACACAGTTACCAGGGCCACTGGCAGCAGGGCAAGCGCGAAGGGCTGGGCGTGGAGCGCAA GAGCCGCTGGACCTACCGCGGCGAGTGGCTGGGCGGGCTGAAGGGGCGCAGCGGCGTATGGGAGAGCGTG TCCGGCCTGCGCTACGCCGGGCTCTGGAAGGACGGCTTCCAGGACGGCTACGGCACCGAGACCTACTCGG ATGGAGGCACTTACCAAGGCCAGTGGCAAGCCGGGAAACGCCACGGCTACGGGGTGCGCCAAAGTGTACC CTACCACCAGGCGGCGCTTCTGCGCTCACCCCGCCGCACCTCCCTGGACTCCGGCCACAGCGACCCCCCG ACGCCGCCTCCACCCCTACCCCTACCAGGAGATGAAGGAGGCAGCCCAGCCTCCGGCTCCCGAGGCGGCT TCGTGCTGGCGGGGCCAGGGGACGCCGACGGGGCGTCTTCCCGTAAGCGCACCCCAGCAGCAGGTGGATT CTTCCGCCGTTCACTGCTGCTCAGCGGGCTCCGGGCTGGTGGGCGCCGCAGCTCCCTGGGCAGCAAGAGG GGATCTCTACGCAGCGAGGTGAGCAGCGAAGTGGGCAGTACAGGACCCCCGGGCTCCGAGGCCAGCGGGC CCCCAATCCCAGCTCCACCTGCCCTCATCGAGGGCTCAGCCACTGAGGTGTACGCCGGTGAGTGGCGTGC GGACCGGCGCAGTGGCTATGGAGTGAGCCAGAGATCCAACGGGCTGCGCTACGAAGGCGAGTGGCTGGGC AACCGACGCCACGGATACGGGCGCACCACCCGGCCCGATGGCTCCCGTGAGGAGGGCAAGTACAAGCGTA ACCGGCTGGTACACGGGGGACGTGTCCGCAGCCTCCTGCCTCTGGCCCTTAGACGGGGCAAAGTCAAGGA GAAAGTGGACAGGGCTGTCGAAGGTGCCCGTCGAGCTGTGAGTGCTGCCCGCCAGCGCCAGGAAATTGCG GCTGCCAGGGCAGCAGACGCCCTCCTAAAAGCAGTGGCAGCCAGCAGCGTCGCGGAGAAGGCTGTGGAGG CTGCGCGGATGGCCAAGCTGATAGCCCAGGATCTACAACCCATGTTAGAGGCCCCAGGCCGCAGACCCAG ACAGGACTCAGGAGGTTCTGACACAGAGCCTTTGGATGAGGACAGCCCTGGGGTATACGAGAATGGACTG ACCCCCTCAGAGGGCTCTCCGGAACTACCCAGCAGCCCCGCCTCCTCCCACCAACCTTGGCGAGCCCCTG CCTGCCGGAGTCCACTGCCTCCTGGAGGGAACTGGGGCCCCTTCTCCAGCCCTAAAGCTTGGCCCGAGGA GTGGGGGGGTCCTGGTGAGCAGGCAGAGGAACTAGCTGGCTATGAGGCTGAGGATGAGGCTGGGGTGCAG GGTCCAGGACCAAGAGATGGTTCCCCACTTCTCGGAGGCTGCAGTGACAGTTCAGGAAGTCTTCGAGAGG AGGAGGGGGAAGACGAAGAGTCCTTGCCCCAACTGAGGGCCCCAGGAGGCTCAGAGTCTGAGCCTGTCAC CACGCCAGTTTTGAGGGGCCTGTCTTCAAGGGGTCCTGATGCTGGGTGCCTGACAGAAGAGGTTGAGGAG CCTGCTCCCACCGAGAGGCCTGCCCAGCCGGGAGCTGCCAACCCCCTGGTGGTGGGAGCCGTGGCCTTGC TGGACCTCAGCCTGGCCTTCCTGTTCTCCCAGCTCCTCACCTGAGGCAACATCCTGGCTTCCTTCGGGTT CTGGCTGCCTGCCTCTGCACCCATTCTGACCTTCGACCTGCCTTCTCTTCTCTCCTCCTCCACCCACTTG TGTTTTCCCCTATCTCCGGGGCCCCTTTCTTTTGGTCTCTCGCTTCTTCCTTCCCTCTATTCTTTGAGAT GGTCTCATTTATTCTGGACCCGTCTCTGGTCTTCCTGTTTTTTCTTTCCCACCCTGACCCCTTCTCTCTC TAGATTGTTTACATATGAAGGGCTGTTCTCTCTCAGAGTTGCTCTCTCCTCTGAGATACACAAATCTAAG TCAGACCATTGCCCCCCCCCATGTCCTCCCCAACCTTTTCTTTGAATCAAAACTTCACCAAGGGTGGGGG TTTGGTGTCCTGAGGAATCTCCTGGAATCTGAGTGGAGGGGAGAAAGGAAATGCATAAGAAGTGATTGGG TGGCCAGACAAGGCACTGCCTGAGCCACTTGGGGCTCACTGGCCCCAAGGGAAAGCTGCAGGAAAAGTAC CCTCCACTCTTACCTCCCAGAACCTACCTAAGTTCTTGCCTACTTTGATCTTCCCTGAGGGAGAAAAGGG GAGCGAACAACAGGAAACTCCCCGTGCTCCCTCTCCTGCCCTCCTCAACCAGTCCCTCTCCCAACCACCA AGGATGGCACGCCAAGCCAAGAGTATTGAGAAAAGCAACGAAAAACCATCTGGTGCAACCAAGCCCGCCC CTCTTCAGATTCTGCCACTCACCTCTCTACCCTTCTCTGCCCCCCTGAAAATTTATTTGGTTGGTCTGGA CTCACTTGTGGCCTTTGATTAAATTCCTAAGGGGACTGAAGAAGACATTTCTGCAGAGGGTTAGAGGCGC TGCAGCAAGCGAGGACCCCAGTCCTCAGCTCTGACAGTTGTGGGGCGAACACGTTGAGGGCTGAAGGGCA GCAGATGCTGTCACAAGTCTAGTTTCTAACCCCTCGCGTAGACTCCTGCGAGATGGGAGCTAACGGGGAA TCAGAGGAAGCCGCTGCCCCACACAGGGCAGGGCAGAGGTGGGTGTCAACCCCGGACGAGAGTTGGGGGG AGAGCCTGTTTGAGATTATGGCTGATCCCTGGGACTAACTTTCCCTCTGGGGACAGAAAGCTGTACAAGA GGGGACTGTGGAACCCCGGGGGACTTTCCTACCCTGCATGAGGCAGAGGCAAGCTGCCTGCCAATCAATC CCTTCCTCAAGGAATGGCCTTGCCCAGGAATGCCCACCACACACACACTCTTCTTATTTTTCTAGTCAAC CTCTGTTTACTCCCTGGCCTGCCTTCCTCCTGCCTCCCTTCCTTCCTTCCTTCTCAATCTTTACTTCTGA TTTCTATTTCATGGAATTTGGGATTGAAGTTATACAACAGTGCCGCCAACATCAAGTCTTGCAGGAAAAA AAAAATAACAAAGAAATTTAACAAAAAAAAGTATATTAATAAAAAAGTTCCAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:6810400 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810400 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7714 same embryo
	EMAGE:7713 same embryo
	EMAGE:7715 same embryo
	EMAGE:7716 same embryo
	EMAGE:7711 same embryo
	EurExpress:euxassay_007469 same experiment
	MGI:4825683 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source