EMAGE mouse gene expression spatial database

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EMAGE:11351

Cyp2c50 cytochrome P450, family 2, subfamily c, polypeptide 50 ( MGI:2147497)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003532. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003532_01	euxassay_003532_02	euxassay_003532_03	euxassay_003532_04

euxassay_003532_05	euxassay_003532_06	euxassay_003532_07	euxassay_003532_08	euxassay_003532_09

euxassay_003532_10	euxassay_003532_11	euxassay_003532_12	euxassay_003532_13	euxassay_003532_14

euxassay_003532_15	euxassay_003532_16	euxassay_003532_17	euxassay_003532_18	euxassay_003532_19

euxassay_003532_20	euxassay_003532_21	euxassay_003532_22	euxassay_003532_23	euxassay_003532_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11351_wholemount_strong.wlz

11351_wholemount_moderate.wlz

11351_wholemount_weak.wlz

11351_wholemount_possible.wlz

11351_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11351_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7412
Entity Detected:	Cyp2c50, cytochrome P450, family 2, subfamily c, polypeptide 50 ( MGI:2147497)
Sequence:	sense strand is shown >T7412 CTCCATGGATCCAATCCTGGTCCTGGTGTTCACCCTCTCCTGTCTGTTTCTCCTCTCACTCTGGAGACAG AGCTCTGAAAGGGGGAAGCTCCCTCCTGGCCCCACTCCTCTTCCAATTATTGGAAATATCCTTCAGATCA ATGTGAAGGACATCTGCCAATCCTTCACCAATTTATCAAAAGTTTATGGCCCTGTATATACTCTATATTT GGGCAATAAGCCCACTGTGGTGTTGCATGGATATGAGGCAGTGAAGGAAGCTCTGGTTGACCATGGGGAG GAGTTTGCTGGAAGAGGAAGGCTCCCAGTTTTTGATAAAGCTACTAATGGAATGGGCATTATATTTAGTA AAGGAAATGTATGGAAAAACACAAGGCGCTTCTCACTCACGACTCTGCGGAATTTAGGCATGGGAAAAAG GAGCATTGAGGACCGTGTTCAAGAGGAAGCACGTTGCCTTGTGGAGGAACTTAGGAAAACCAATGGCTCA CCCTGTGATCCCACCTTCATCCTGGGCTGTGCTCCCTGCAATGTCATCTGCTCAATTATTTTCCAGGATC GTTTTGATTATAAAGATCGGGATTTTCTTAATTTGATGGAAAAACTGAATGAAATAACCAAGATAATGAG CACCCCTTGGTTGCAGGTTTGCAATACATTTCCTGTTCTGCTTGATTATTGTCCAGGAAGTCATAACAAA GTTTTTAAAAATTATGCTTGTATTAAAAATTTCCTTTTGGAGAAAATAAAAGAACATGAAGAATCATTGG ATGTTACAATTCCTCGGGACTTTATTGATTATTTCCTAATTAATGGAGGCCAGGAAGACGGCAACTATCC ATTGAAAAATAGGCTTGAACACCTGGCAATAACAGTGACTGATCTTTTTTCTGCTGGGACAGAGACAACA AGCACAACACTGAGATATGCTCTCCTACTCCTGTTGAAGTACCCACATGTCACAGCTAAAGTCCAGGAAG AAATTGAGCATGTGATCGGCAAGCACCGAAGCCCATGCATGCAGGACAGGAGCCGCATGCCCTATACTGA TGCCATGATTCATGAGGTCCAGAGATTCATTGACCTTGTCCCCAACAGCCTGCCCCATGAAGTGACCTGT GACATTAAATTCAGGAACTACTTCATCCCCAAGGGGACAAATGTAATAACATCACTGTCATCAGTGCTGC GTGATAGCAAAGAGTTCCCCAACCCAGAGAAGTTTGACCCTGGGCACTTTCTAGATGAGAACGGAAAGTT TAAGAAAAGTGACTACTTCATGCCCTTCTCAACAGGAAAACGGATATGTGCAGGAGAAGGCCTGGCACGC ATGGAGCTGTTCCTATTCCTAACCAGCATTTTACAGAACTTCAACCTGAAACCTCTGGTTCACCCAAAGG ACATAGATGTAACCCCAATGCTCATTGGATTGGCCTCAGTGCCTCCTGCTTTCCAGCTCTGCTTCATTCC TTCCTGAAAATCAAATGCCTGGCTCCATCTGGGACATCTTCTGTGATCACCCTGAGGCTTTAATTCACTG CTTTCCACACTGGACATACTGCTTTTTATCCATTTCTTTTGCCTGCTCATAATTTCATAAGGTTCTATGA ACATACATTTACCAATTTGCAGATCTTGCACAAATATTTCTGACTGGTCCTATTTTCTGAAACACTGTTA CCATATGCTGTGATGATATGGACCTGATTCCAAGTTAAATATACTCTCATAAAATTGAAATTACTATTGT AAGATAATTCACTGTCGCTTCTCTTCTGCATTTTCTTAATAATAAATTCTTTTGGTATAACTAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4195223 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4195223 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11354 same embryo
	EMAGE:11353 same embryo
	EMAGE:11350 same embryo
	EMAGE:11349 same embryo
	EMAGE:11352 same embryo
	EurExpress:euxassay_003532 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source