EMAGE mouse gene expression spatial database

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EMAGE:11352

Serpina3k serine (or cysteine) peptidase inhibitor, clade A, member 3K ( MGI:98377)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003558. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003558_01	euxassay_003558_02	euxassay_003558_03	euxassay_003558_04

euxassay_003558_05	euxassay_003558_06	euxassay_003558_07	euxassay_003558_08	euxassay_003558_09

euxassay_003558_10	euxassay_003558_11	euxassay_003558_12	euxassay_003558_13	euxassay_003558_14

euxassay_003558_15	euxassay_003558_16	euxassay_003558_17	euxassay_003558_18	euxassay_003558_19

euxassay_003558_20	euxassay_003558_21	euxassay_003558_22	euxassay_003558_23	euxassay_003558_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11352_wholemount_strong.wlz

11352_wholemount_moderate.wlz

11352_wholemount_weak.wlz

11352_wholemount_possible.wlz

11352_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11352_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 12 13 14 15

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 12 14 17

pons mantle layer

moderate

regional

moderate expression: see section 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 19 20 21 22 23 weak expression: see section 24

dorsal root ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 09 10 11 12 13 17

axial skeleton

strong

regional

strong expression: see section 13 14 15 16

axial skeleton tail region

strong

regional

strong expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7414
Entity Detected:	Serpina3k, serine (or cysteine) peptidase inhibitor, clade A, member 3K ( MGI:98377)
Sequence:	sense strand is shown >T7414 TGAACATCAGGAGTCAGCTATCACAGAGGCTCTGCAGCCTGGAGAACAGAGAAGATGGCCTTCATTGTAG CTATGGGGATGATCTTAATGGCTGGAGTCTGTCCTGCTGTCCTATGCTTCCCAGATGGCACAAAGGAAAT GGACATTGTATTCCATGAACACCAAGACAATGGGACACAAGATGACAGTCTCACATTGGCCTCCGTCAAC ACTGACTTTGCCTTCAGCCTGTACAAGAAGCTGGCTTTGAAGAATCCAGATACAAATATTGTCTTCTCCC CACTTAGCATCTCAGCTGCCTTGGCCCTTGTGTCCCTGGGAGCAAAGGGCAAGACCATGGAAGAGATTCT AGAAGGCCTCAAGTTCAATCTCACAGAGACCCCTGAAGCAGACATCCACCAGGGCTTTGGCAACCTCCTA CAGAGTCTCAGCCAGCCAGAAGACCAGGATCAGATAAACATAGGCAATGCCATGTTTATTGAAAAGGACC TGCAGATCCTGGCAGAATTCCATGAGAAGACAAGGGCTCTGTACCAGACTGAGGCCTTCACAGCAGACTT CCAGCAGCCTACTGAGGCCAAAAACCTCATCAATGACTATGTGAGCAATCAGACCCAGGGAATGATCAAG GAACTCATCTCAGAACTGGATGAGAGGACATTGATGGTGCTGGTGAATTACATCTACTTTAAAGGCAAAT GGAAGATATCCTTTGACCCCCAGGACACATTTGAGTCTGAGTTCTACTTGGATGAGAAGAGATCTGTGAA GGTTCCCATGATGAAAATGAAGTTACTGACCACACGCCACTTCCGTGATGAGGAGCTATCGTGCTCTGTG TTGGAGCTGAAGTACACAGGAAATGCCAGCGCCCTGCTCATCCTCCCTGACCAGGGCAGGATGCAGCAGG TGGAAGCCAGCTTACAACCAGAGACCCTGAGGAAATGGAGGAAAACTTTGTTTCCCAGCCAAATAGAGGA GCTAAACCTGCCCAAGTTCTCCATCGCTAGTAACTACAGGCTGGAGGAGGATGTCCTTCCAGAAATGGGG ATTAAGGAAGTCTTCACCGAACAAGCTGACCTATCTGGGATCACAGAAACCAAGAAACTGAGTGTGTCTC AGGTGGTCCACAAGGCTGTGCTGGATGTGGCTGAGACAGGCACAGAAGCAGCTGCTGCCACAGGGGTTAT TGGTGGCATTCGTAAGGCCATATTACCAGCTGTGCATTTCAACAGGCCATTCCTGTTTGTTATCTATCAC ACAAGTGCTCAGAGTATCCTCTTTATGGCCAAAGTCAATAACCCCAAGTAGACCTAAAGCTCACCAAGTT CTCATGGTTCTGCCCAGGGCTCTGGGACAAAGTCTGCATGTGGATCTCTATATACATCCTGTCATTCAAG CTCTGATTGGCTGTTGCAAAGTTGGCTTAGACAGCTACATTAACTATCTCTGTGATCCCACATGCACATA AGACTTTGGACTCTCAGTGTCAGGCTCTCGGTTGTCTTGGGAGCATCTGTGCATATTTCTGAACCTGGAA TCTGCCTTTATTCTTCTTCCCGGGTGACTCCTCTTTATGTGTCTGTACCCCAAACCTAGGCATTTGCAAA TACACAGAGTTCCAATACCGACAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4195356 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4195356 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11354 same embryo
	EMAGE:11351 same embryo
	EMAGE:11353 same embryo
	EMAGE:11350 same embryo
	EMAGE:11349 same embryo
	EurExpress:euxassay_003558 same experiment
	MGI:4827980 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source