EMAGE mouse gene expression spatial database

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EMAGE:11353

Cyp4f14 cytochrome P450, family 4, subfamily f, polypeptide 14 ( MGI:1927669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003534. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003534_01	euxassay_003534_02	euxassay_003534_03	euxassay_003534_04

euxassay_003534_05	euxassay_003534_06	euxassay_003534_07	euxassay_003534_08	euxassay_003534_09

euxassay_003534_10	euxassay_003534_11	euxassay_003534_12	euxassay_003534_13	euxassay_003534_14

euxassay_003534_15	euxassay_003534_16	euxassay_003534_17	euxassay_003534_18	euxassay_003534_19

euxassay_003534_20	euxassay_003534_21	euxassay_003534_22	euxassay_003534_23	euxassay_003534_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11353_wholemount_strong.wlz

11353_wholemount_moderate.wlz

11353_wholemount_weak.wlz

11353_wholemount_possible.wlz

11353_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11353_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7411
Entity Detected:	Cyp4f14, cytochrome P450, family 4, subfamily f, polypeptide 14 ( MGI:1927669)
Sequence:	sense strand is shown >T7411 CGGACGCGTGGGTGCTTTGCTAGCTCTGGTCATTCCCCTCACCAGCATACCCCTCCTCCCCCTATTCATC TTCTTCTCCACTCCTGTACCTCTTCACCCAACATTTCCTTGGTTCTCAAGTCTCCTTTCTGCTCTATCCT CTGTCCAGTGTCTTGAGATTATTGTCCCTTTCCGGGACCTCAGTTACTCACTGCACATTTATTCACCCAG CAGGATGTCACAGCTGAGCCTGTCCTGGTTGGGACTGGGGCCCGAGGTGGCCTTCCCATGGAAGACCCTG CTACTGCTTGGGGCCTCCTGGATCCTGGCCCGGATTCTGATCCAGATCTATGCTGCCTATAGGAACTATC GTCACCTCCATGGCTTCCCTCAGCCCCCCAAACGGAACTGGCTTATGGGTCACGTGGGCATGGTAACTCC TACAGAGCAGGGCTTGAAGGAATTGACTCGTTTGGTGGGTACCTACCCCCAAGGTTTTTTGATGTGGATT GGACCAATGGTCCCTGTTATTACTCTATGCCATTCTGACATCGTCCGATCTATCCTCAATGCCTCAGCCG CTGTTGCACTCAAGGATGTGATATTCTACAGCATCCTAAAGCCTTGGCTGGGGGATGGGCTCTTGGTGAG TGCTGGTGACAAGTGGAGCCGCCATCGTCGCATGCTGACACCCGCTTTCCATTTCAACATCCTGAAGCCC TATGTGAAGATTTTCAATGATAGCACCAACATCATGCACGCCAAGTGGCAACGGTTGATTTCAGATGGCA GTGCCCGTCTGGACATGTTTGAGCACGTCAGCCTGATGACTCTGGATAGTCTGCAGAAATGTGTCTTCAG CTTTGACAGCAACTGTCAGGAGAAGTCCAGTGAATATATTGCTGCCATCCTGGAGCTCAGTGCCCTAGTG GCCAAAAGGCACCAGCAGCCTTTGATGTTCATGGACCTGCTGTATAATCTCACCCCTGATGGTATGCGCT TCCGTAAGGCCTGCAACGTGGTGCATGAATTCACTGATGCTGTCATCCGGGAGCGGCACCGCACCCTCCC AGATCAGGGTTTGGATGACTTCCTTAAGTCTAAGGCCAAGTCCAAGACTTTGGATTTCATTGATGTGCTG CTGCTGAGCAAGGATGAAGATGGAAAGGAGCTGTCAGATGAGGACATCCGAGCAGAGGCTGACACCTTCA TGTTTGAGGGCCATGACACGACAGCCAGTGGACTCTCCTGGATCCTCTACAACCTGGCAAGGCACCCAGA ATACCAGGAGCGCTGCCGGCAGGAAGTTCAGGAGCTGCTGAGGGGCCGAGAGCCTGAGGAGATTGAATGG GACGACCTGGCCCAGCTACCCTTCCTGACCATGTGCATCAAGGAGAGTCTGCGACTGCATCCCCCAGTCA CGGTCATCTCCCGATGCTGCACCCAGGACATTCTGCTCCCAGATGGCCGGACCATTCCCAAAGGTATCAT CTGTCTCATCAGCATCTTTGGAATTCATCACAACCCATCCGTGTGGCCAGACCCTGAGGTCTATGACCCC TTCCGCTTTGACCCTGAAAACATCAAAGACAGCTCACCTCTGGCATTTATTCCCTTCTCGGCGGGACCCA GGAACTGCATAGGACAGACTTTCGCCATGAGTGAGATGAAGGTGGCACTGGCGCTGACGCTGCTGCGCTT CCGACTCCTACCGGATGACAAGGAGCCGCGCAGGCAGCCAGAGCTGATCCTGCGCGCAGAGGGCGGGCTG TGGCTGCGGGTGGAGCCGCTGAGCGCGGGCGCTCATTGACCTAAGACCTGGTCTAGAGCCACCCTACCAA CATATCTCTGCGGATCCCAGAATGAACAGAACTGTCACCCATACCTGAATCTTACCGAGAGCCAGCAGGG GGCGCTGTGAAATCCAGTGCGTGGGTGAGAGAGGCCTGCTCCATAAGGTGCATTCTCCTCCAACCAACCT GGACTATTCTCTGAACAACATAGGAAGCCAAAGGGATGTCTGAGCAAGAGGCCAGAATCAAAGGCAAATT TGAGCTTTGATTCATGCAGCTCTGTTTCCATTACTTTGACTTCCATTCTAATCACCTAATAGACCATTCT TCTCTCACCTCAGAATTACTTTTCTGTGGGCACTAAATTACTGTGTTTATTAAACCTTAAGACCGGAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4195156 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4195156 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11354 same embryo
	EMAGE:11351 same embryo
	EMAGE:11350 same embryo
	EMAGE:11349 same embryo
	EMAGE:11352 same embryo
	EurExpress:euxassay_003534 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source