EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11551

Kpnb1 karyopherin (importin) beta 1 ( MGI:107532)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006809. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006809_01	euxassay_006809_02	euxassay_006809_03	euxassay_006809_04

euxassay_006809_05	euxassay_006809_06	euxassay_006809_07	euxassay_006809_08	euxassay_006809_09

euxassay_006809_10	euxassay_006809_11	euxassay_006809_12	euxassay_006809_13	euxassay_006809_14

euxassay_006809_15	euxassay_006809_16	euxassay_006809_17	euxassay_006809_18	euxassay_006809_19

euxassay_006809_20	euxassay_006809_21	euxassay_006809_22	euxassay_006809_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11551_wholemount_strong.wlz

11551_wholemount_moderate.wlz

11551_wholemount_weak.wlz

11551_wholemount_possible.wlz

11551_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11551_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7946
Entity Detected:	Kpnb1, karyopherin (importin) beta 1 ( MGI:107532)
Sequence:	sense strand is shown >T7946 CCCGAGCAGCAGCCGCGCTCCGAGCTGCCCCCGGGGTCCCTCCCCCGCCGCCAGAGGAGCAGCTGCTGCC GCCTAGCACATTAGGAGGGCGGAAGGAAGGAAGGAGAGCGGGGAGGAAGGGGAGCCGGAGCGCCTGGCCA GAGGCAGCCGGTGAATGGGCTTGTGGTGACCCCCACCCCCCACCCCACCCTCCCTTCCCACCCGACCCCC AACCCCCATCCCCAGTCGGAGCCGCCGCCCGACAGGCCGGGCCGCCGTCTTGCGAGGAGTCCCCGCCGCC GTCACCTCCGCCATGGAGCTCATAACCATCCTCGAGAAGACCGTGTCTCCGGATCGGCTGGAGCTGGAAG CGGCGCAGAAGTTCCTCGAGCGTGCGGCCGTGGAGAATCTGCCCACGTTCCTTGTGGAACTGTCCAGAGT GCTGGCAAACCCAGGAAACAGTCAGGTTGCCAGAGTTGCAGCTGGTCTACAAATTAAGAACTCTTTGACA TCGAAAGATCCAGATATCAAGGCACAATACCAGCAGAGGTGGCTCGCTATTGATGCTAATGCTCGACGGG AAGTCAAGAACTATGTTTTGCAGACGTTGGGCACAGAAACGTACCGGCCTAGTTCGGCCTCACAGTGTGT GGCTGGTATTGCTTGTGCAGAGATCCCAGTAAGCCAGTGGCCAGAGCTAATTCCTCAGCTGGTAGCCAAT GTCACAAACCCCAACAGCACAGAGCATATGAAAGAGTCCACATTGGAAGCTATTGGTTACATTTGCCAAG ATATAGACCCAGAGCAGCTACAGGATAAGTCCAATGAGATCCTGACTGCCATAATCCAGGGGATGAGGAA GGAGGAGCCTAGTAACAATGTGAAGCTGGCTGCTACCAATGCACTCCTGAACTCACTAGAGTTCACCAAA GCAAACTTTGACAAAGAGTCTGAAAGGCACTTTATCATGCAAGTGGTCTGTGAAGCCACACAGTGTCCAG ACACAAGGGTAAGAGTGGCTGCTTTACAGAATCTAGTGAAGATAATGTCCTTGTATTACCAGTACATGGA GACATACATGGGTCCTGCCCTTTTTGCAATCACAATTGAAGCAATGAAAAGTGACATTGATGAGGTGGCT CTCCAAGGGATAGAGTTCTGGTCCAATGTCTGTGATGAGGAAATGGATTTGGCCATTGAGGCTTCAGAGG CAGCAGAGCAAGGACGCCCCCCGGAGCACACCAGCAAATTTTACGCCAAGGGAGCACTGCAGTACTTGGT GCCCATCCTCACACAGACACTGACTAAACAGGATGAAAACGATGACGACGATGACTGGAACCCTTGCAAA GCAGCTGGGGTGTGCCTCATGCTCCTGTCCACCTGCTGTGAAGATGACATTGTGCCGCATGTCCTTCCCT TTATTAAAGAGCACATCAAGAACCCTGACTGGCGATACCGGGATGCAGCAGTGATGGCTTTTGGCAGTAT CTTGGAAGGACCAGAGCCTAATCAACTGAAACCATTAGTCATACAGGCTATGCCCACCCTAATAGAACTA ATGAAAGACCCCAGTGTAGTTGTTCGAGACACAACAGCGTGGACTGTGGGCAGGATCTGTGAGCTGCTGC CTGAAGCCGCCATCAACGATGTCTACCTGGCACCCCTTTTACAGTGTCTGATTGAGGGCCTCAGTGCTGA GCCCAGGGTGGCTTCAAATGTGTGCTGGGCTTTTTCCAGTCTGGCTGAAGCTGCGTATGAAGCTGCAGAT GTAGCTGATGATCAAGAAGAACCAGCCACCTATTGTCTGTCTTCTTCCTTTGAACTTATAGTTCAGAAGC TATTGGAGACCACCGACAGACCCGATGGACACCAGAATAACCTGAGAAGCTCTGCGTATGAGTCTCTCAT GGAAATCGTAAAGAACAGTGCCAAGGATTGTTACCCTGCCGTGCAGAAGACCACCCTGGTCATTATGGAA CGGCTGCAGCAGGTGCTTCAGATGGAGTCCCATATCCAGAGCACATCCGACAGAATCCAGTTCAATGACC TCCAGTCTCTACTCTGCGCGACTCTTCAGAATGTTCTCCGGAAAGTGCAGCATCAAGATGCTCTGCAGAT CTCTGATGTGGTCATGGCCTCCCTGTTAAGGATGTTCCAAAGCACAGCTGGGTCTGGGGGAGTGCAAGAA GATGCCCTGATGGCAGTTAGCACACTGGTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCT TTAAACCATTCCTGGGCATTGGACTGAAAAATTATGCTGAGTACCAGGTATGTTTGGCAGCTGTTGGCTT AGTTGGAGACTTGTGCCGAGCCCTGCAGTCTAACATCTTGCCTTTCTGTGACGAGGTGATGCAGCTGCTC CTGGAGAACTTGGGGAATGAGAATGTCCACAGGTCTGTGAAGCCACAGATTCTGTCTGTGTTTGGTGATA TTGCTCTTGCCATTGGTGGAGAGTTTAAAAAATACTTAGAGGTTGTATTGAATACTCTACAGCAGGCCTC CCAAGCCCAGGTTGACAAGTCAGACTTTGACATGGTGGATTATCTGAATGAGCTAAGAGAAAGCTGCTTG GAAGCTTATACGGGAATCGTCCAGGGATTGAAGGGAGATCAGGAAAACGTACACCCGGATGTAATGCTGG TACAGCCCAGAGTAGAATTTATTTTGTCTTTTATTGATCACATTGCTGGAGATGAGGATCATACGGACGG AGTGGTAGCCTGTGCTGCTGGTCTGATAGGGGACTTGTGTACAGCCTTCGGGAAGGATGTACTGAAGTTA GTAGAAGCTAGGCCAATGATCCATGAACTATTAACTGAAGGGCGGAGATCGAAGACTAACAAAGCAAAGA CCCTCGCTACGTGGGCAACCAAGGAACTGAGGAAACTGAAGAACCAGGCTTGATCTGGTACCACTGGGAT GATGACCTGAGACCCCCACTGGAAATCTCCAATCTTTTGAAAAACCCGGAAGTGAGGAGTGTGCACGGAT GCTGAATGCTTGGGAATGAGAGGATGAGTGAGTGAGGCTTGAAAACACCACACTGAAAATCCTGCCACAG CCAACAGCAGCAGCGCTGTTAGACATGAGCTAAGTAAGCACTGAGTTTGTAGAAAACATAACATCAGCCA TCTTGGAACAGAGAAACAATGGATTTACTTACTTATTAAAAACAAAAAAGCTGTCTCTTCCCAGGGAAGG CTAGAACTAGCTTTCCTGTCTTGGCCGGTGCCGAATGGGTGACTGATTTGGGAGAGGAGGACCTGGGTTC ACCTGTGGGGCTGTGTTGTGAAAGGCAGCCTGGCCCCTGCTACTGAGGAGAAAGATGGAGCCCGGGTCTT GAGCCCACCTTCACTGTACCGTTGCACTTGGTACTGTCTATGTGCCCTTGAGAAGGCGCGTGGGGGATTT CACAGTCTGAGAATGAGTGTGTGTGAGTGAGGCGGTACCCACATTCTCAACTTCAAGTCATTGCAGTTTC TTTTTCCCAGAAAACAAAGGGGTTTGATGTTGCATTTCATAAACTAACTGAAGTTCTGGCTACTGATGCA GCACAAGAGACGTGTTTTTTTTTTTAAATGAGGAAAGACGCTGTTTAGGTTTGGTTTGGTTTCATTTTAA TTACTCTAGGGAAAACACTGACGAATGGTCAGCGCTCCTGTCCTGATCTTTTCATCAAGGCGCCTTTCCT GTACTATGGTTCAGCTGTGAATGTAGCTATGGGGGGAGGGGGGAGAAAGGAGAACTCTGGAGTTAGAGGA TATAGAAAATAAAAGTACAGTTGTTACAAAGAAAATGCAGACTTCAAAGACAGAAAAAGCCACAACCCAA ACCAAAATTTAAATGCTTGAAATTGGCATCATAAACAAGCTCTCTCCTGACTTGTATTGTGGCAGTCGGA ATGCCCCCAGAAAACTGTGCCAAAGAGTTTAGAAAACAAATGTACAATAAAAGTAAATACACTCAAAACC GCAAACTTCAGGTAACTATTTTGAATTGCAAATGACGAAAATTTAATGTTGAACGATCTGATAAAATAAC CATTTGGAAACTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6518750 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6518750 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11556 same embryo
	EMAGE:11553 same embryo
	EMAGE:11552 same embryo
	EMAGE:11554 same embryo
	EMAGE:11555 same embryo
	EurExpress:euxassay_006809 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source