EMAGE mouse gene expression spatial database

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EMAGE:11555

Wipf1 WAS/WASL interacting protein family, member 1 ( MGI:2178801)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006807. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006807_01	euxassay_006807_02	euxassay_006807_03	euxassay_006807_04

euxassay_006807_05	euxassay_006807_06	euxassay_006807_07	euxassay_006807_08	euxassay_006807_09

euxassay_006807_10	euxassay_006807_11	euxassay_006807_12	euxassay_006807_13	euxassay_006807_14

euxassay_006807_15	euxassay_006807_16	euxassay_006807_17	euxassay_006807_18	euxassay_006807_19

euxassay_006807_20	euxassay_006807_21	euxassay_006807_22	euxassay_006807_23	euxassay_006807_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11555_wholemount_strong.wlz

11555_wholemount_moderate.wlz

11555_wholemount_weak.wlz

11555_wholemount_possible.wlz

11555_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11555_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7924
Entity Detected:	Wipf1, WAS/WASL interacting protein family, member 1 ( MGI:2178801)
Sequence:	sense strand is shown >T7924 GCCCTGCGGACCCAGAGAGAAGTTTCCCAGAAAAAAATGCCCCGCGCGCCGCGGGGCCGAGGAGCCGCCC GGAGCCGCTGCACGATTTACCTTCAAGACTGCTGAAATTTTAACTACCCAAGATGCCTGTCCCTCCCCCA CCAGCACCCCCACCGCCACCAACATTTGCACTCGCTAACACTGAGAAGCCCACCTTGAACAAGACAGAGC AGGCTGGGAGGAATGCTCTTCTCTCTGACATCAGCAAAGGGAAGAAGCTAAAGAAGACGGTCACCAATGA CAGAAGTGCCCCAATATTGGACAAACCAAAGGGAGCCGGCGCCAGTGCTGGTGGCTATGGTGGAGGCGGC GGAGGTGGTGGTGGAGGAGGAGGAGGAGGAGGAGGCAGCGGTGGAAATTTTGGAGGGGGTGGTCCTCCAG GACTGGGAGGACTATTCCAGGCTGGAATGCCGAAGCTGAGGTCCACCGCCAACAGGGATAATGATTCTGG AGGAAGCCGACCACCGATTTTGCCACCGGGAGGAAGAGCCACATCTGCCAAGCCCTTTTCACCCCCGAGT GGCCCGGGGAGGTTTCCCGCACCCTCCCCAGGCCACAGGAGTGGGCCCCCAGAGCCACCCAGGAACAGAA TGCCTCCCCCGAGGCCTGACGTGGGCTCAAAACCAGATAGCCTTCCCCCTCCGGTACCAAATACGCCAAG ACCTGTTCCGTCGAGTCTGCACAACCGGGGATCCCCAGCAGGGCTGGGAGCCCCCAGGCCCCCCTTTCCC GGGAACCGAGGTGCTGCTTTTGGAGCAGGCTCTGCGCGCCAGAACCCCTCCGGCTCCTCCTCTCCGTTTC CTAGGCCCCCTTTGCCCCCCACCCCAAGCCGAGCCTTGGATGACAAGCCCCCTCCACCACCTCCTCCCGT GGGCAACAGGCCCTCCATGCACAGAGAAGCGGTGCCCCCTCCCCCCTCACAGACCAGCAAACCTCCAGTG CCTTCCACCCCGCGGCCTGGGTTGGGTTCGCAGGCACCACCTCCTCCTCCACCGCCCAGCCGGCCAGGGC CCCCTCCTCTGCCTCCAGCTTCCAACGACGAGATCCCCAGGCTGCCACAGCGGAACCTGTCCCTCACGTC CTCTGCACCTCCATTGCCTTCACCTGGACGCTCAGGGCCTCTCCCTCCCCCACCCAGCGAGAGGCCCCCT CCTCCTGTAAGGGATCCACCAGGCAGATCAGGCCCCCTCCCACCACCACCTCCAATAAACAGAAATGGAA GCACAGCTCGGGCCCTGCCTGCCACCCCTCAGTTGCCATCCAGGAGTGGAATGGACAGTCCCAGAAGTGG GCCTAGGCCTCCTCTTCCTCCTGACAGGCCTGGTGCTGGGGCGCCGCCCCCACCCCCACCCTCCACATCA GTTCGAAATGGCTTCCAAGACTCATCGTGTGAAGATGAGTGGGAAAGCAGATTCTACTTCCATCCGATTT CTGACTTGCCGCCTCCAGAGCCATACGTACCGACAACCAAGACGTATCCCAGCAAACTGGCCAGAAACGA AAGCCGAAGTGGATCCAACAGAAGAGAAAGGGGCGCCCCACCCCTTCCTCCCATCCCGAGGTGATCTCTG CCTGCTCCGTTCTACCCAAGCTCCAAAGCTGCTTCTGTTGTTGCTGTTTGAAAACTGCACACCCCTCCTC TCGATTTGCCACGCCCCCCCCAGAGAGAGTAGGAGGGAGGAACGGAAGCTGAGGAGGGGATCCCTGCGTG TGGGGGTGGGGATCGGGGAAAGAAGCGCCTAGCTGTTTATGTTGTATATATTCCTGAGCTATTGCTTGTA TCAGCTGCAGCCTGCCAGGGTCAGACATCCACTGCTTTTTTGCAACAGACTGTAGTTGTTAAATCCCCTG AGAGCTGGTTTTCTCTATGCCTTTCTTGCTCACATGGCCTCTGGCCTGTTTCCACGAACCACACCGCCCA AGCGAGTTGCGAGCAAGCCCTGTGGTTACAGAACGTCCGATCTTCGGCTGTCTGAGGAAGGCTCCAGGAC AGAGCTGTCTTAAAAGAAACATAATGTGAGCCCCATACCCTGTCTGAAAATGACTAGCAGCTATGTTTTA TAAAGGTAGAAGGAAACAGATGAAAAGAATTGCATTTTACTCAGCACTGTGCATCTGAAATATCACTTCG ACTTGTATTATTAATGAGCTATTTACATTTTTGGTACTAAGTCCTCACACTCGAGTAACCATTCTGTACT TGGAACAAGCACACTGCCAGATTTCAAGTGTCCACACCGGCCTCAGCACCACACTGTCAGACGGTCTGGA AGACAGGAGTTAGGACAGCTTGTGCCTAAAAAGGCAAATTGAGTTTTAGCAATTCAGGAGATGTCCCAAG ACGGAGTCAGAACTTGATACAATGTTTGGCAGGCAGTGAGACCTTAGATTTTTGACCCTTATTCTGCCTT TGTCCTTAATATGTATGTCCAGGTGTTTCTTCAAAGTATCTTAGTGAGAAATGTATCTAGTAGAGTTTAC AGTCCCGTTTACAGTGGATTGCAAACTCCACTGCAAGAAAACAAAACTGTGTGGGTACTCCCTGGCTTAG AACCAGCTGGCTTGTGCACTGCTGGTCACTGGCCTTGCGCATCTTCTGTGGCTCTTCAGTCCTCCACCCC AGAGAAAACTCCTTCCTCAGGTCAGGAAAGGCTAGTAGGGCAAGATAGGAGAAGAAAGTCAGAGAATGTA TCAGAACTATGGGAGAAGAGGATAGCGTTGATGATGGGCAGAGCTCTACACAACCGACTGGGGTCAAGTA GATTAAGGAGCTTACCAGTGGAACCTAGAAAGCATTGGTGTTGAATTTCTTGGGAGAATCATTGTCTTGG ATCTGTGCAAGTAAGTTCCACTAAAGGATGGATCACAAAAAGGGCCAGGCTTGCCTTTTTTATATATATG ACTTTGTTTACAACATTTTACTGGGACTTTCAAACCTAGCTGTAGAGTTGGAAAAAGATTTCCATTTTGA TGTTTTATATAATCATGTTTAATGTTACCATTGTCTATTAAAGCACATGGCCACATATGTGTACCTACCT GAACTTTGTATTTTGGGTCCAGCGTATTGCTCACATGTTGCTGTCACATGTTGCTGAAAGATGCTAATAA AAAAAAATCAATCCAAAGAAAAATGGAAAGACATTCCTATTTATAGAATGATTGTTTCGTTTATATAAAA GCATAACTTAGGAGTTCTAATAGATGAAAAGTCTAATAAATTATAAAGTGATTGATTTGAATATATTGTA TTTTTATAACTTTCCTTGCCAAAGCCCAGACCTAGTTCTTTAGAGAGTAGTTTTCCTCCCTTCTCCTCCA CCATTTGTCGGTCTTCCATGAGAACAAAGAAATAAAAGGGTTAACAAGCTTGACAATTTTTCTATTCCTA ACATTTTAAAATGTATTTCGAATTCTTTGATCTGATATTCACTAGGATAAACATCCTTTTCATAGACGCT CAATGACCTACTTTACTGAAAGTCGAATTGGTCCTTCCTTGTGCTGTGTATGGAAGAGAAAGGTTGGACT CAAAGATTGTGGGTCTCAGTTTTTTAAGGGATAGTGAAAATTGGACAAGTTCTGGTAAATAAATCTCAGT TTTCAGGTACATTTCTAAGTCAGGGACGGGTCACAGCTCAAAGTTGCCATTCATAATAAAGAAGGTTTAG AATGCATTTTGCAAAGAATGTTTCATTAATACACCTACATCCTATCTTTGGAAGTCAGTGACCCAATTGC ATTTAAAACTCTTGAGAAGTTCTGGGTTTAGCTTAACCTATGGTAAATCATTTAAAAAAAAAAAGTCTAT TTTCTTCCCTGAAATCAAAGCTTACATGTATGTTTTTCCATAATATTTCCAGTTATGTGTATTTAAGATG CAATGCAGTATTTTAACATTTGCAAATCATTTTATATGGAAATATATTGCAGTATTAAAATTCAGTGTTC TGTGTCTATTTCACTATGCATTTTATTTACTGAAAGCTGAGAAAATATTTAACCCAATGGTGCCTTACTG GTGATCTGAAAAGAATTGACTTGTTATGTCTAAGTAGTGGATTTGTTGCATATTTGTAAAAACTGTTAGG CCTTTATATTTTAACTTGTAATATCGATTTTGTTATTTTAGTACCAGAAACAGGGTGATTATTGAAGTCT CCTCAAACTTTTGGTATGAAATTTAACTTTCCCTGTTCATAGGCAGGAGCTGTAGAGGAATTTTTTCATA CCACTGCACTGTGTAAACAGACACAGACATCTGTGTCATCAGGGTAGTTAAATGGTAGGGTAGAATCTAC CATAGACACCTGCATGTTTGTAAATTATCCAGACAATGAATAAAAAGCAGAATGATAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6514406 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6514406 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11556 same embryo
	EMAGE:11553 same embryo
	EMAGE:11552 same embryo
	EMAGE:11554 same embryo
	EMAGE:11551 same embryo
	EurExpress:euxassay_006807 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source