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EMAGE:11554

Mrpl35 mitochondrial ribosomal protein L35 ( MGI:1913473)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554
"Pseudo-wholemount" of euxassay_006802. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006802_01 euxassay_006802_02 euxassay_006802_03 euxassay_006802_04
EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554
euxassay_006802_05 euxassay_006802_06 euxassay_006802_07 euxassay_006802_08 euxassay_006802_09
EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554
euxassay_006802_10 euxassay_006802_11 euxassay_006802_12 euxassay_006802_13 euxassay_006802_14
EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554
euxassay_006802_15 euxassay_006802_16 euxassay_006802_17 euxassay_006802_18 euxassay_006802_19
EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554 EMAGE:11554
euxassay_006802_20 euxassay_006802_21 euxassay_006802_22 euxassay_006802_23 euxassay_006802_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11554Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11554_wholemount_strong.wlz
11554_wholemount_moderate.wlz
11554_wholemount_weak.wlz
11554_wholemount_possible.wlz
11554_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11554_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8223
Entity Detected:Mrpl35, mitochondrial ribosomal protein L35 ( MGI:1913473)
Sequence:sense strand is shown

>T8223
CCGTAGGCAGGCTGCAAGATGGCGGCTTCCATCTTCACGGGCGCCGTGAGAGCTGCCTCAGGAATATTTC
GTCCCTTGAATGTCTTGGCATCTTCAACCTATCGGAACTGCGCCAGGAATGCCTGTCTTAATTCTTCACT
GTGCACCATTCATTTTAGACATATTCAGACATCAGTTGTTTCCTCTGCTCCAAGACTTGTCACATCTGTT
GGACACCTGGCATATGGGCATACCACAACAGTCCTTAATAGAGTGGCCACATTGGTTCCCAGTGTCCTGA
AGCCACCAGTCAGAGCTCTAACATACTGCAGCACACGGAAAGGCAAGAGAAAGACGGTAAAGTCGGTCGT
GCATCGCTTCCTCCGACTTCATTCTGGCCTTTGGCTAAGGAGAAAGGCTGGCTATAAGAAAAAATTATGG
AAAAAGTCAACTGCAAGAAAGAAGCGCTTGAGGGAATTTGTGTTCTGCAGCAAGACCCAGAGTAAACTCC
TGGATAAAATGACGACATCTTTCTGGAAGAGGCGCAACTGGTACGCTGGCGATCCTTACCAGATGTACCA
CGATCGAACAAACCTACGAGTATAGTTCAGAGCTATCATAGTTCCCAGCTATGGAATGTGGGTCTTTGTG
TACATAATGCCCTTGCAAAGTGAGTATGCGTTAAACTGTAAACTGTACCAGCTGATCGGCAAGCTTAGAT
TACCACCATTGTGCATTAAACATTCCCAGAGTTGGTCATAGAACTAAAAGTTAAATTTATTGTGCATTAT
TTCAAAAAACTGGTAATGGCTTTCCTTATTTCTGGAATCTTGGATGAGGAAAAACACGAAAAGACGCTCA
ACAGTGGGCCTTTGTGTGGAATTGAGATGGTATCCAGGTTTTTGCACATGCTAAACAGACATCTTACATG
CCTATTCCCAATATGACACATGCTGTGTTTAAAAAATAAATACCTAGGAACTCAGAAAAAAAAAAAAAAA
AAAAAAA
Notes:The probe template was PCR amplified from IMAGE:1245736 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1245736 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11556 same embryo
 EMAGE:11553 same embryo
 EMAGE:11552 same embryo
 EMAGE:11555 same embryo
 EMAGE:11551 same embryo
 EurExpress:euxassay_006802 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS