EMAGE mouse gene expression spatial database

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EMAGE:11552

Cml2 camello-like 2 ( MGI:2136446)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006794. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006794_01	euxassay_006794_02	euxassay_006794_03	euxassay_006794_04

euxassay_006794_05	euxassay_006794_06	euxassay_006794_07	euxassay_006794_08	euxassay_006794_09

euxassay_006794_10	euxassay_006794_11	euxassay_006794_12	euxassay_006794_13	euxassay_006794_14

euxassay_006794_15	euxassay_006794_16	euxassay_006794_17	euxassay_006794_18	euxassay_006794_19

euxassay_006794_20	euxassay_006794_21	euxassay_006794_22	euxassay_006794_23	euxassay_006794_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11552_wholemount_strong.wlz

11552_wholemount_moderate.wlz

11552_wholemount_weak.wlz

11552_wholemount_possible.wlz

11552_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11552_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adenohypophysis

weak

spotted

weak expression: see section 12 13 14 15 16 17

kidney calyx

moderate

regional

moderate expression: see section 22 23 weak expression: see section 07 08 09 10 11 12 19 20 21

left lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

right lung

moderate

regional

moderate expression: see section 16 17 18 19 20 21 22 23 24

trachea epithelium

moderate

regional

moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7383
Entity Detected:	Cml2, camello-like 2 ( MGI:2136446)
Sequence:	sense strand is shown >T7383 CCACGCGTCCGCAATAAAGTGCCAGACCAGATCTGCACCCTAGAGGTTCACCAGGCTCTGGTAGGTTTTA CTGGATGTCATCGGAGGCAAAGGCCATCCTGGACATTTGGATCTGTCATATTAGACTGAATCATTCCAGT TGCTGGAAAGAGGATTTGTTGAAACTTGGACCTGGGAACACAGGAGTTTTCAACTCTGGGCCCTGAAGAG GAAACAGAAGATCTCAGAACAGCACATCTTTCCACAGTGTAGAACCTCAGTTCCCAAAGGGCTCAGGGAA GTTATGCAAGAAGGTCTGGATGTCCCTTGTGATCACTGATACTTGAGAGCCAGAAGTCTCCCCATGGCTG CTTATCACATCCGACAGTACCAGGAGAAGGACCACAAAAGGGTCCTGGAATTGTTCTCCAGCGGCATGAA GGAGCTTATTCCTGCTGCCATCCGACAGATGCTGACACTGCCTCATTCTCTCTTGCTCTTACCTGGAGTG CCTGTGACCATAGTATTGATGTCTGCCTCCTGGCTCCTGGCCACATTATACAGCTTCCTCTTTCTCCTTT GCCTGTGGCTTATTTTCTGGATTTCTTGCAGAAATTATGTGGCTAAAAGTTTGCAGGCAGATCTTGCTGA CATCACCAAGTCTTACCTGAATGCACATGGCTCCTTCTGGGTGGCTGAGTCTGGAGACCAGGTAGTTGGC ATGGTGGGTGCTCAGCCAGTCAAGGACCCTCCATTAGGGAAGAAGCAGATGCAGCTCTTTCGCCTGTCTG TGTCCTCACAGCATCGAGGACAGGGAATAGCAAAGGCACTGGTCAGAACTGTCCTCCAGTTTGCTCGGGA CCAGGGTTACAGTGATGTTGTCCTTGAGACTGGCAGTGTGCAACATAGTGCTCAGGCTCTCTACCAGGCC ATGGGCTTCCAGAAGACAGGCCAGTACTTTGTCAGTATAAGCAAGAAGTTAATGGGTCTTTCTATTCTTC AATTCTCTTACTCTCTCCCTTTTGCTTCAGGACCAGGGTATAGTGGGAAATATTTAAAAAAAGGTCCCAT TCCATGCTAGCACCAGGTACTCTCTGGCCCCAGTGGTCTCACTGCCTCCATGGCTTGTCCTATGTAGCAA CTGCCCATCTTGAGGATCTCTTGCTGGGGATTGTAACCTGGTAAAATCAAAAGTCTAGAGGCTTGTGATT TATCAGTCAGATTTAAATTAATAAATTCTCAACCCACAAAATGTCCCCACAATGAATTCAAAACTCAATT GAAATAAACTGAGGCTACACACCTAGATGGGGCAAACATACCCTATTTATTATTTAATCATCTATCCAAT AAATTCCCAATATATATGGCTCCCAGGACTGTGTGCTTCTGGCTTCTCATCCTCTCCTATAGTTTTGATT TTGTCTCCTGCCCTGTGTCTCTTCTTCTCTCTAATATTTCCTGTTACACAAAGGATGTGATCCTTTTTTG ATTTGTGTGTCCAGAAGGCTTCAGTTCACTCTGCTCTAGGAACAAGCCACTCCTGGCAGTTTCCCAGTAC CAATCCCAAGGGCTCCCTTTGCACTCCTTTCAGATGCAGCCATGCTGGTGGTGACCTGTGGGTGTTGGCT CCATTCTGCCTTTTTAAGTATCTCTCTTTCGTCTTTCTAGTGCCATGATAAGATAGCACAAAACCCAACT GAGTCCATCCTGCACACAGTTCACTTAGGAATCTGTTAGCTGTCAGTATCAGCTAGGTGGTCTCAAGACT TCAAGGCAAGCAACCTTGCTGAGCTGAAGTTCCAGCAGCTCACAGGATTTAAATCTAAGACTTGTGTAGA AATGGAGCCTCCACTGGTAGAGGCACAGCACAGATCAGGGTGGCTGCACTGAGAGCACTGGAGCAACGAA GCCTGTAACTTACACCTAAGCCACCTCTGTGCAGAGGGAGGAAGGGGACCTCTGATGCAGTGAGAGTAAC TTTGAATGGCCTCTCCTGGTGGAATGATCCCTCCTGGCCTCTTGGTCCCAGTCTTATGGGAAAGCACAAT TAAATGCATGAAATGTACTTGGGGATTCATGAAGATGAAATGGGATCAGGAATTGTATGAATATGTCACC CTAGGGAAAATGCCTGGACCAACTGGCACTCGAGTAGGTTGGACATCAGTGGGCTTCCAGTGCTCCTGGA TTTCCTCTGTAAAAAGTTAAAATGGCAGTTCAGGAGTTGGAACAGCACTGTAGAGTGGAGAAAGGCAGTC TCAGAACTAATAAAGTTGTTGGCTGAAGATGACCCTGCTAGGCATAAGAGACTTCCAGAATTGTTAAGGA AAGGCCCAGAGGGACATCAAATCACAGATGTGGTTTCCACTGCTTTTGGTTGCATGTTGGAACTGGAGAA CTCCTTGCTGAGGACATGACCTGTTTGGTTTCAGGACACAGAAGAATCAACAGAGAGTGACCTGGAACAG CCTTGTGTGCTCGCTGGGTTTCTAGTGCCAAGAGGCTCGATCCAGGCTGCTGAGGGACAATTGTAGAGAC AGTCTTGCTCCCTGTGCTGTGTTGTTACTTGCCGGGAAGATGTATGTTGTAGTGTTATAGTGGCATTGTT ATGTTTGCACACACATACTTTCTGATTAAGGCCTCCATAGGTGGATATTCCCATTGATTATTCTAAATCA AGACAAAATTATGTGATGTGCAGGCCACAGGCCATAGTAAGAAGACTAGTACTATTGTTTTAGTTTTTAT TAAGCAGATATAAACTTTTATCAGCTTGCCTTCATTTCTTTTTATGCCCAATGACTGCTGTACCAGCATC ACTTGTAAAGGGAGCCTTTTTCACAGTGAGAGGTAGTCACTCCAGAGACTCATAATTTCCCTGATTGCTG AGCATAACTGACTGATCCTTTGATATAATCCGATATTGGGTCATAAATGGAAGAAAATGGATATACACAA ACACACACACACACACACACACACATCACCTGCTCCAAGGCTTGGAAGAGTTATGGAAGCGGATGCATAA AGATTTAAGATCTGGAGTGGGTGGGATACCCTGTATCTCTCTGGTCTGAATTGAAACTTCCCCTTTTAGG GGGCTGACAGAGGCCCAGGAGACTCTGGAGAGCGTTCCTCTCTGATGTCACCCAGGCAGTAAACATTTAT CAGCAGAGAGAAGCCTCTTAGGAACCCCAGGGTTGGAGGTTTGTAAGTCATTACCAATGCAATGGCCATA CTGCCTGGGCAACCCGTGCTCCTGTAAGTAATCCCTCAGCTGTGCTCCTGTGCTCCAATAAACTGTTTCA TCAAACTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4193874 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4193874 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11556 same embryo
	EMAGE:11553 same embryo
	EMAGE:11554 same embryo
	EMAGE:11555 same embryo
	EMAGE:11551 same embryo
	EurExpress:euxassay_006794 same experiment
	MGI:4823945 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source