EMAGE mouse gene expression spatial database

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EMAGE:13629

Pgpep1 pyroglutamyl-peptidase I ( MGI:1913772)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019252. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019252_01	euxassay_019252_02	euxassay_019252_03	euxassay_019252_04

euxassay_019252_05	euxassay_019252_06	euxassay_019252_07	euxassay_019252_08	euxassay_019252_09

euxassay_019252_10	euxassay_019252_11	euxassay_019252_12	euxassay_019252_13	euxassay_019252_14

euxassay_019252_15	euxassay_019252_16	euxassay_019252_17	euxassay_019252_18	euxassay_019252_19

euxassay_019252_20	euxassay_019252_21	euxassay_019252_22	euxassay_019252_23	euxassay_019252_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13629_wholemount_strong.wlz

13629_wholemount_moderate.wlz

13629_wholemount_weak.wlz

13629_wholemount_possible.wlz

13629_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13629_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 18 19 20 21

pancreas

moderate

regional

moderate expression: see section 10 11 13 14 18 19 weak expression: see section 12

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 16

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 14 15 16

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 11 17 18 weak expression: see section 10

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 18 19 20 21 22 23 moderate expression: see section 13 14 15 16 17 24

pons mantle layer

weak

regional

weak expression: see section 08 19

pons ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20

ventral grey horn

moderate

regional

moderate expression: see section 13 14 17 18 weak expression: see section 12 15

spinal cord ventricular layer

strong

regional

strong expression: see section 13 14 15 16

esophagus

moderate

regional

moderate expression: see section 14

metanephros

moderate

regional

moderate expression: see section 08 09 10 21 22 weak expression: see section 18 19 20

testis

strong

regional

strong expression: see section 06 07 21 22 moderate expression: see section 08 09 18 19 20

lung

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T799
Entity Detected:	Pgpep1, pyroglutamyl-peptidase I ( MGI:1913772)
Sequence:	sense strand is shown >T799 TCCTCGAGNCTGTTGGCCTACTGGAGAGAGTGCATTCCACCATGGAGCAGCCGCGGAAGGCGGTAGTGGT AACCGGATTCGGCCCTTTTGGGGAGCATACTGTGAATGCCAGCTGGATCGCTGTCCAGGAGCTGGAGAAG CTGGGCCTCGGGGACAGCGTGGACCTGCATGTGTACGAGATCCCCGTGGAATACCAGACGGTGCAGAGGC TCATCCCAGCACTGTGGGAGAAGCACAGCCCCCAGCTCGTAGTGCATGTTGGGGTGTCGGGCATGGCCAC CACAGTGACGCTGGAAAAATGTGGGCACAACAAGGGTTACAAAGGACTGGATAATTGCCGGTTCTGCCCC GGCTCTCAGTGCTGCGTGGAGGATGGTCCCGAGAGCATCGACTCCATCATCGACATGGACGCCGTGTGCA AAAGGGTGACCACACTGGGACTGGACGTGTCTGTTACCATCTCCCAGGATGCTGGCAGGTATCTGTGTGA CTTCACGTATTATACATCGCTCTACCAGGGCCGCGGCCGCTCCGCTTTTGTCCACGTGCCC
Notes:	The probe template was PCR amplified from IMAGE:1921042 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1921042 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13634 same embryo
	EMAGE:13633 same embryo
	EMAGE:13632 same embryo
	EMAGE:13630 same embryo
	EMAGE:13631 same embryo
	EurExpress:euxassay_019252 same experiment
	MGI:4827177 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source