EMAGE mouse gene expression spatial database

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EMAGE:13631

Sox12 SRY-box containing gene 12 ( MGI:98360)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019555. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019555_01	euxassay_019555_02	euxassay_019555_03	euxassay_019555_04

euxassay_019555_05	euxassay_019555_06	euxassay_019555_07	euxassay_019555_08	euxassay_019555_09

euxassay_019555_10	euxassay_019555_11	euxassay_019555_12	euxassay_019555_13	euxassay_019555_14

euxassay_019555_15	euxassay_019555_16	euxassay_019555_17	euxassay_019555_18	euxassay_019555_19

euxassay_019555_20	euxassay_019555_21	euxassay_019555_22	euxassay_019555_23	euxassay_019555_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13631_wholemount_strong.wlz

13631_wholemount_moderate.wlz

13631_wholemount_weak.wlz

13631_wholemount_possible.wlz

13631_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13631_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 06 07 weak expression: see section 22 23

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 09

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 weak expression: see section 08 09 10 19 20 21 22 23 24

neural retina

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55184
Entity Detected:	Sox12, SRY-box containing gene 12 ( MGI:98360)
Sequence:	sense strand is shown >T55184 CAGTCTGATGCCAGGACTGAATAAAGTGTATTTGCCCCGACCTTGCCCTGTGGTTCTGCATGTGTACTTC TCACCCTGGCCAGCTTCAAAGTGCCTAGAGTATGGGCTGGTGCAGGGAGAACGGGTTCTCTTTACCCCTG GCCAGCTTCAAAGTGCCTAGAGTATGGGCTGGTGCAGGGAGAGCGGGTTCTCTTTAGGCCTCTGTTATCC TCTATCTCAGGAATCCCTGCCTTCATTAAGAATGGCAAACTCATCTTCACAGTGGCTAAGCATCGTAACT GGGAGGACTGGGGTGGGTGCTGATGTCACAGTGCAAGGGTGCAAGCCTCTGTAGACAGCCATATATTCCA AGAGATGCAGGAAACCAGTCATTGTGCTTGTTAAGTCTCATTAGGTCAGACGAGACAAGCATGCATGCAG GCAGGACTGATCATCCGGGGTCAATTCCACTGTTTAATAAAGGATTGGGAAGCAAAATTGGTAGCTTTGC TCAGAGCCTATGTTCTGAGCAGCTCTACAGTGTGTGTCTTCTAAGGATCAACATGGTATTTTCAGTGGGA AACCATGCTTGAGGTAAGCAGTATTTAAGGTGGTGTGCTCCATAAATTGTGTTCCTTCATTTCCAGAGTA GGAAGCTGGGGAGAGGGGTCCCCATCAAGCAACCATGGCTAGAAGTATTCTGAGGTATCCCTCAAATGTG TTGTCCCATGCTGAGAGGTGGCCTAGGACCTGACTTACAAGGGCCTGCAGTGTAAGCCTACCACAGGACC AGAGGGCAACAGACCGAGAGTTTTCTCCTGAGGTTCTACTCAGTTCTTCCTCGGCGCATTCGTTGTACCT TTCTCCCCCATGCGTTGCCTTGTCCAGTGAATGTCACTGCTCAAGAAACGCCCATTTTCCTAATAAGCCA GGACTGGGTGTGGATCTTTTCAGGGCATCTTCTCTGCTTCTGGTTGGAAGCAAACTGGGAGGAAATCATG AGTAGATAACATTGAGAAAGGGAGGACAGGGCCTGTGCCAAACAGAAGTCAGTTCAAGGAAGGGTTTCTT TCCC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGTCTGATGCCAGGACTGA; Reverse Primer - name:unspecified, sequence:GGGAAAGAAACCCTTCCTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13634 same embryo
	EMAGE:13629 same embryo
	EMAGE:13633 same embryo
	EMAGE:13632 same embryo
	EMAGE:13630 same embryo
	EurExpress:euxassay_019555 same experiment
	MGI:4828389 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source