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EMAGE:13634

Adat2 adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae) ( MGI:1914007)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13634 EMAGE:13634 EMAGE:13634 EMAGE:13634 EMAGE:13634
"Pseudo-wholemount" of euxassay_019250. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019250_01 euxassay_019250_02 euxassay_019250_03 euxassay_019250_04
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euxassay_019250_05 euxassay_019250_06 euxassay_019250_07 euxassay_019250_08 euxassay_019250_09
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euxassay_019250_10 euxassay_019250_11 euxassay_019250_12 euxassay_019250_13 euxassay_019250_14
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euxassay_019250_15 euxassay_019250_16 euxassay_019250_17 euxassay_019250_18 euxassay_019250_19
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euxassay_019250_20 euxassay_019250_21 euxassay_019250_22 euxassay_019250_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13634Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13634_wholemount_strong.wlz
13634_wholemount_moderate.wlz
13634_wholemount_weak.wlz
13634_wholemount_possible.wlz
13634_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13634_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2279
Entity Detected:Adat2, adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae) ( MGI:1914007)
Sequence:sense strand is shown

>T2279
TGGCCTCGAGCCAGATTCGTCGACAGGGNCCGGAGTCTCTGAGCGGCGGAGGTATGGAGGAGAAGGTGGA
GTCCACCACTACTCCAGACGGCCCGTGCGTGGTGTCCGTACAGGAGACGGAAAAGTGGATGGAGGAGGCG
ATGCGAATGGCCAAAGAAGCTTTGGAAAATATTGAAGTTCCTGTTGGCTGCCTTATGGTCTATAACAATG
AAGTTGTGGGGAAGGGAAGAAATGAAGTGAATCAGACAAAAAACGCTACTCGGCATGCTGAAATGGTGGC
CATTGATCAGGTCCTAGACTGGTGTCATCAGCATGGACAGAGCCCTTCCACTGTATTCGAGCACACCGTG
TTGTACGTCACTGTGGAGCCGTGCATCATGTGTGCTGCCGCCCTGCGCCTTATGAAAATCCCGCTGGTCG
TCTATGGCTGTCAGAACGAACGGTTTGGTGGTTGCGGTTCCGTCCTAAACATTGCCTCTGCTGACCTACC
CAATACTGGAAGACCGTTTCAGTGCATCCCTGGGTACCGAGCTGAGGAGGCTGTGGAGCTCCTAAAGAC
Notes:The probe template was PCR amplified from IMAGE:1095573 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1095573 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13629 same embryo
 EMAGE:13633 same embryo
 EMAGE:13632 same embryo
 EMAGE:13630 same embryo
 EMAGE:13631 same embryo
 EurExpress:euxassay_019250 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS