EMAGE mouse gene expression spatial database

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EMAGE:13633

Pld4 phospholipase D family, member 4 ( MGI:2144765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019253. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019253_01	euxassay_019253_02	euxassay_019253_03	euxassay_019253_04

euxassay_019253_05	euxassay_019253_06	euxassay_019253_07	euxassay_019253_08	euxassay_019253_09

euxassay_019253_10	euxassay_019253_11	euxassay_019253_12	euxassay_019253_13	euxassay_019253_14

euxassay_019253_15	euxassay_019253_16	euxassay_019253_17	euxassay_019253_18	euxassay_019253_19

euxassay_019253_20	euxassay_019253_21	euxassay_019253_22	euxassay_019253_23	euxassay_019253_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13633_wholemount_strong.wlz

13633_wholemount_moderate.wlz

13633_wholemount_weak.wlz

13633_wholemount_possible.wlz

13633_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13633_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 13 14 15 17 moderate expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T491
Entity Detected:	Pld4, phospholipase D family, member 4 ( MGI:2144765)
Sequence:	sense strand is shown >T491 TCTCGAGNCTGTTGGCCTACTGGTGCAGACAGTGGCTGGAACAGGATGGACAAGAAGAAAGAGCACCCAG AGATGCGGATACCACTCCAGACAGCAGTGGAGGTCTCTGATTGGCCCTGCTCCACATCTCATGATCCACA TAGCGGACTTGGCATGGTACTGGGGATGCTAGCTGTACTGGGACTCAGCTCTGTGACTCTCATCTTGTTC CTGTGGCAAGGGGCCACTTCTTTCACCAGTCATCGGATGTTCCCTGAGGAAGTGCCCTCCTGGTCCTGGG AGACCCTGAAAGGAGACGCTGAGCAGCAGAATAACTCCTGTCAGCTCATCCTTGTGGAAAGCATCCCTGA GGACTTGCCATTTGCAGCTGGCAGCCCCACTGCCCAGCCCCTGGCCCAGGCTTGGCTGCAGCTTCTTGAC ACTGCTCGGGAGAGCGTCCACATTGCCTCGTACTACTGGTCCCTCACTGGACTGGACATTGGAGTCAATG ACTCGTCTTCTCGGCAGGGAGAGGCCCTTCTACAGAAGTTCCAACAGCTTCTTCTCAGGAACA
Notes:	The probe template was PCR amplified from IMAGE:1481227 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1481227 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13634 same embryo
	EMAGE:13629 same embryo
	EMAGE:13632 same embryo
	EMAGE:13630 same embryo
	EMAGE:13631 same embryo
	EurExpress:euxassay_019253 same experiment
	MGI:4827260 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source