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EMAGE:13723

Dmrta2 doublesex and mab-3 related transcription factor like family A2 ( MGI:2653629)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723
"Pseudo-wholemount" of euxassay_019535. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019535_01 euxassay_019535_02 euxassay_019535_03 euxassay_019535_04
EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723
euxassay_019535_05 euxassay_019535_06 euxassay_019535_07 euxassay_019535_08 euxassay_019535_09
EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723
euxassay_019535_10 euxassay_019535_11 euxassay_019535_12 euxassay_019535_13 euxassay_019535_14
EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723
euxassay_019535_15 euxassay_019535_16 euxassay_019535_17 euxassay_019535_18 euxassay_019535_19
EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723 EMAGE:13723
euxassay_019535_20 euxassay_019535_21 euxassay_019535_22 euxassay_019535_23 euxassay_019535_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13723Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13723_wholemount_strong.wlz
13723_wholemount_moderate.wlz
13723_wholemount_weak.wlz
13723_wholemount_possible.wlz
13723_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13723_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55032
Entity Detected:Dmrta2, doublesex and mab-3 related transcription factor like family A2 ( MGI:2653629)
Sequence:sense strand is shown

>T55032
GCCACCGACGGCGGGGGTCCTGGAGCTGGAGCGCCCGCGGGGAGTGCAGGGGGCGCAGGGGGCGCAGAGG
CCAAATTGCAGAAGTTTGATCTGTTTCCCAAGACGCTGCTTCAGGCCGGCCGCCCCGACAGTCCGCAGCC
GCCGCCAGGGAAGCCCTTGTCGCCCGACGGCGCGGACTCAGGTCCCCGGACCTCGTCCCCGGAGGTGCGG
CCCGGCTCAGGCTCGGAGAACGGAGACGGCGAGTCCTTTTCCGGGTCGCCTCTGGCCCGGGCCTCCAAGG
AGGCGGGTGGCAGCTGTCCTGGGAGCGCGGGCGCCGGCGGTGGCGGCGAGGAGGACAGCCCCGGCTCCTC
CAGCCCCCTGGG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCCACCGACGGCGGGGGTCC; Reverse Primer - name:unspecified, sequence:CAGGGGGCTGGAGGAGCCGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13727 same embryo
 EMAGE:13724 same embryo
 EMAGE:13726 same embryo
 EMAGE:13725 same embryo
 EurExpress:euxassay_019535 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS