EMAGE mouse gene expression spatial database

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EMAGE:13724

Pappa2 pappalysin 2 ( MGI:3051647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019547. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019547_01	euxassay_019547_02	euxassay_019547_03	euxassay_019547_04

euxassay_019547_05	euxassay_019547_06	euxassay_019547_07	euxassay_019547_08	euxassay_019547_09

euxassay_019547_10	euxassay_019547_11	euxassay_019547_12	euxassay_019547_13	euxassay_019547_14

euxassay_019547_15	euxassay_019547_16	euxassay_019547_17	euxassay_019547_18	euxassay_019547_19

euxassay_019547_20	euxassay_019547_21	euxassay_019547_22	euxassay_019547_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13724_wholemount_strong.wlz

13724_wholemount_moderate.wlz

13724_wholemount_weak.wlz

13724_wholemount_possible.wlz

13724_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13724_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 09 10 17 18 19

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 06 21 22 23

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 07 18

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 17 18 20 21 22 weak expression: see section 08 09 19

dorsal root ganglion

moderate

single cell

moderate expression: see section 09 11 12 13 16 17

mandible

moderate

regional

moderate expression: see section 20 weak expression: see section 09 10 11 12 17 18 19 21 22

lower jaw incisor

moderate

regional

moderate expression: see section 13 14 16 17 weak expression: see section 18

lower jaw molar

moderate

regional

moderate expression: see section 09 10 weak expression: see section 19

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 16 17 weak expression: see section 18

upper jaw molar

moderate

regional

moderate expression: see section 09 weak expression: see section 19

clavicle

moderate

regional

moderate expression: see section 11 weak expression: see section 10 12 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55137
Entity Detected:	Pappa2, pappalysin 2 ( MGI:3051647)
Sequence:	sense strand is shown >T55137 TCCCTTGGCTCCAGTATTTGAAGAAACTTAAGTCCCTAATAAAGAAAATGATCAGAGATCCTAGGAACAA GTCATGTTTGCCCCCAGGTGGTTGTATTCAGTTCTCCTTAGACATCCATAATGTCATCCAGGGCCCTCCA CAGAGGCTCCTCTGCCTGTTTCTTTATTACAAAGTTACCTGTCTATTTCTAGTTCCTGGTAACCACAGTT TTGAGATCAACATGGCCCAAAGGTGGCTGGCTTCAATAGTAGCATGGCAGCCTTCAATCACAGATACAGA AAAGAGATCAGCAGGGGCAAAGAGCAGATCTGGGCAAATAAGGCAGCGAGTAGAGTCTTCTTGGTTCTTT TCTTTCCTTTCCAACATTTAAGGCCTTAGTCTTTGACACATCATCTATTCTGTGTGATCACCATTGAGTG TGTCAGTTAGCACCCAAGGGGACAGTTTGATGGCAAACACAGTGAAAGGGGCTG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCCCTTGGCTCCAGTATTTG; Reverse Primer - name:unspecified, sequence:CAGCCCCTTTCACTGTGTTT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13723 same embryo
	EMAGE:13727 same embryo
	EMAGE:13726 same embryo
	EMAGE:13725 same embryo
	EurExpress:euxassay_019547 same experiment
	MGI:4827043 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source