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EMAGE:13727

Mef2d myocyte enhancer factor 2D ( MGI:99533)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727
"Pseudo-wholemount" of euxassay_019542. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019542_01 euxassay_019542_02 euxassay_019542_03 euxassay_019542_04
EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727
euxassay_019542_05 euxassay_019542_06 euxassay_019542_07 euxassay_019542_08 euxassay_019542_09
EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727
euxassay_019542_10 euxassay_019542_11 euxassay_019542_12 euxassay_019542_13 euxassay_019542_14
EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727
euxassay_019542_15 euxassay_019542_16 euxassay_019542_17 euxassay_019542_18 euxassay_019542_19
EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727 EMAGE:13727
euxassay_019542_20 euxassay_019542_21 euxassay_019542_22 euxassay_019542_23 euxassay_019542_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13727Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13727_wholemount_strong.wlz
13727_wholemount_moderate.wlz
13727_wholemount_weak.wlz
13727_wholemount_possible.wlz
13727_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13727_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55105
Entity Detected:Mef2d, myocyte enhancer factor 2D ( MGI:99533)
Sequence:sense strand is shown

>T55105
GAGCTGGATGGGCTCTTCAGGCGCTATGGGTCATCTGTTCCGGCCCCCAACTTTGCCATGCCTGTCACAG
TGCCCGTGTCCAATCAGAGCTCCATGCAGTTCAGCAATCCAAGTAGCTCTCTGGTCACTCCTTCCCTGGT
GACATCATCCCTTACGGACCCACGGCTCCTGTCCCCCCAGCAGCCAGCACTACAGAGAAACAGTGTTTCT
CCAGGCTTGCCCCAGCGGCCTGCTAGTGCAGGAGCCATGCTGGGTGGAGACCTCAACAGTGCTAATGGAG
CCTGCCCCAGCCCCGTTGGGAATGGCTATGTCAGTGCCCGAGCTTCCCCTGGCCTCCTCCCTGTGGCCAA
TGGCAACAGCCTAAACAAAGTCATCCCTGCCAAGTCTCCGCCCCCACCCACCCACAACACCCAGCTTGGA
GCCCCCAGCCGCAAGCCTGATCTGCGGGTCATCACTTCCCAGGGAGGCAAAGGGTTAATGCATCATTTGA
ACAATGCCCAGCG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GAGCTGGATGGGCTCTTCA; Reverse Primer - name:unspecified, sequence:CGCTGGGCATTGTTCAAA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13723 same embryo
 EMAGE:13724 same embryo
 EMAGE:13726 same embryo
 EMAGE:13725 same embryo
 EurExpress:euxassay_019542 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS