EMAGE mouse gene expression spatial database

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EMAGE:14151

Krt79 keratin 79 ( MGI:2385030)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001839. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001839_01	euxassay_001839_02	euxassay_001839_03	euxassay_001839_04

euxassay_001839_05	euxassay_001839_06	euxassay_001839_07	euxassay_001839_08	euxassay_001839_09

euxassay_001839_10	euxassay_001839_11	euxassay_001839_12	euxassay_001839_13	euxassay_001839_14

euxassay_001839_15	euxassay_001839_16	euxassay_001839_17	euxassay_001839_18	euxassay_001839_19

euxassay_001839_20	euxassay_001839_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14151_wholemount_strong.wlz

14151_wholemount_moderate.wlz

14151_wholemount_weak.wlz

14151_wholemount_possible.wlz

14151_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14151_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 04 05 06 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4450
Entity Detected:	Krt79, keratin 79 ( MGI:2385030)
Sequence:	sense strand is shown >T4450 ATGGAACCCAGGGCCAGCCCAGCTGGGAAAAGATAAAAAGGTGTTTGGTGGCTCAGTAGCTCACTCCGGC TGATTCCCTCCTGCTCTCAGCTGCTTTCTCGCTGACAATCATGCGGTCCTCTTTGTCCAGGCAGACTTTC TCCACTAAAGGAGGCTTCAGCTCTAACTCAGCCAGCGGAGGTGGTGGGAGTCGGATGCGAACCAGCTACA GCTCAGTGACTATGTCCAGGGGGAGCGGCGGTGGCGGTGGGGTCCGCTCTGGGTCCAGCTCCGGGGGCTT TGGCAGCCGGAGCCTCTACAACTTGGGAGGCAAGAATATCTCCGTCAGCATGGCTTGTGGGGCCTCCTCT GGCCGGGCACTAGGAGGCTTTGGCAGTGGGGCCTATGTGGGCCTAGGGGCTAGCAGGCAGACATTTGGGC CAGTCTGTCCTCCCGGGGGCATCCAGGAAGTCACTGTCAACCAGAGCCTGCTGACGCCTCTCAATGTGGA GATAGACCCCGAGATTCAGCGGGTGCGCACCCAGGAGCGCGAGCAGATCAAGACCCTCAACAACAAGTTT GCCTCCTTTATTGATAAGGTACGCTTCCTGGAACAACAGAACAAGGTGCTGGAGACGAAGTGGGCGCTGC TGCAGGAGCAGAGCCAGAACACGGGGGTCGCCAGGAGCCTGGAGCCTTTCTTTGAGAATTACCTGAGCAC CCTGAGGAGGCAGCTGGATACCAAACAGAGCGAGCGGGGCCGGCTGGATATGGAGCTGAGGAACGTACAG GATAATCTGGAGGACTTCAAGAACAAGTATGAGGATGAAATCAACAAACGCACTGCCCTGGAGAATGAGT TTGTGCTGCTCAAGAAGGATGTAGATGCCGCATACATGGGCAGAATGGATCTGCATGGCAAAGTGGACAG CTTGACACAAGAGATTGACTTCCTGCAACAGCTCTTTGAGATGGAGCTGAGCCAAGTGCAGACCAATGTG TCTGACACCAACGTGATACTGTCCATGGACAACAACCGCAACCTGGACTTGGACAGCATCATCGCTGAAG TCAAAGCCCAGTATGAGCTGATCGCCCAGAAGAGTCGGGCTGAGGCTGAGTCCTGGTACCAGACCAAATA TGAAGAACTGCAGGTGACAGCTGGGAAGCACGGGGACAGCCTTCGAGACACTAAAAATGAGATCGCAGAA CTCACTCGCACCACTCAGAGACTGCAGGGCGAGGTGGATGCAGCCAAGAAGCAGTGCCAGCAGCTGCAGA CAGCCATCGCAGAAGCAGAGCAGAATGGGGAGATGGCGCTCAAGGACGCTAAGAAGAAGCTTGGAGATCT AGACACAGCCCTGCATCAGGCCAAGGAGGACCTGGCCCGGATGCTGCGTGAATACCAGGATCTTGTGAGC GTCAAGCTGGCCCTGGATATGGAGATCGCCACCTACCGCAAGCTGCTGGAGAGCGAGGAGAGCAGGATGT CTGGAGATTGTCCCAGCGCAATCAGCATCTCGGTGACCGGCAACTCTACCTCCGTTTGCGCAGGCGGCAC GGCTGGCTTCGGGAACGGCCTCTCTCTGGGCGGGGCTGGCGGGGCCAGCAAGGGCGGCTTTGGTTCTAGT GTGAGCTATGGCGCTGCCAAAGGCGGTCAGGTCTCTGGGGGAACCTCCATCCTACGGAAGACCACTACAG TCAAGACCTCTAGCCGGAGATACTAGCAGCAAGGCTTTGAGAGTTGGGCCTTGGTCCTTGCGCTGGTCAT GGCTTCTCAGGACCGCAGAGGCTGAGTATTTGTAGTGCACAATTTACATATAATTTGCATTTGGGAAAGG GCTCAAATCTACTCAGGTTTTTCTCCTTGGTTATGCAGTGGAATGGGAGGGAAGTTAGGGTCACCAGCTG GTTGATTTGGGGGTGACCTGACCATTTAGAGGGGGTAGGATGTCCAGTTCTACGTTTTCACCTCAGCAGA TCTGCTGAGTTCAGGGCAAGCTGGAGGAGGCAGAAGGGGTTGCCAGAGACCTGCAGCTCCTTATCCTTGT CACCTCACGGTCAAGTCCCTGTCTGTGCCTGTGATGTGTCTCAATAAACAACAACTACCTACCGCAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4935395 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4935395 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14155 same embryo
	EMAGE:14153 same embryo
	EMAGE:14150 same embryo
	EMAGE:14152 same embryo
	EMAGE:14154 same embryo
	EurExpress:euxassay_001839 same experiment
	MGI:4825847 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source