EMAGE mouse gene expression spatial database

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EMAGE:14154

Eif2d eukaryotic translation initiation factor 2D ( MGI:109342)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001856. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001856_01	euxassay_001856_02	euxassay_001856_03	euxassay_001856_04

euxassay_001856_05	euxassay_001856_06	euxassay_001856_07	euxassay_001856_08	euxassay_001856_09

euxassay_001856_10	euxassay_001856_11	euxassay_001856_12	euxassay_001856_13	euxassay_001856_14

euxassay_001856_15	euxassay_001856_16	euxassay_001856_17	euxassay_001856_18	euxassay_001856_19

euxassay_001856_20	euxassay_001856_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14154_wholemount_strong.wlz

14154_wholemount_moderate.wlz

14154_wholemount_weak.wlz

14154_wholemount_possible.wlz

14154_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14154_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4451
Entity Detected:	Eif2d, eukaryotic translation initiation factor 2D ( MGI:109342)
Sequence:	sense strand is shown >T4451 GCCGAGTCTCAGCATGGCTGCCTCCAGGGTTGAGGGTCCGGGAGCCGCCACTCCTTCGTTTTGTTGACCG CCCCTGGCGTCTGTGCTCTTCTTGTCTTTCCTGAGCTACTCCAGCAGACATGTTCGCTAAGGCCTTTCGG GTCAAGTCCAACACAGCCATCAAGGGCTCGGACAGGAGAAAGCTCCGGGCCGATGTGACAGCTGCTTTCC CTGCCCTGGGAACAGATCAGATCTCTGAATTGATACCTGGAAAGGAAGAGCTAAATGTTGTGAAGTTGTA TGTTCACAAAGGGGATTCGGTGACTGTGTACACGAGTGGTGGTAACCCCATCCTATTTGAACTGGAGAAA AATTTGTATCCAACAGTGTACACACTGTGGGCCTATCCGGATATTCTACCAACTTTTATAACATGGCCTC TGGTATTGGAAAAACTGGTCGGGGGAGCAGATTTGATGCTGCCAGGGGTAGTGGTGCCCCCTACTGGTCT TCCTCAAGTACAGCAAGGCGACCTCTGTGCCATTGCCTTGGTGGGGAACAGAGCGCCCGTTGCAATTGGA GTGGCCGCCATGTCCACAGCTCAGATGCTGGCTTCAGGCCTGAAGGGAAAAGGTGTCTCTGTGCTCCACA CTTATCAGGACCACTTGTGGAGATCTGGAGACAAGTCCTCTCCACCTGCCATTGCCCCACTGGACCCCAC AGATAGCTGCGAAGAGAAGGTGCACCTTGGTCTGCAGGGAAACCTGAAGAGCCTGACCCTGGACGGAGAG GAGGAGAACGGGCAGGTTCCTCTCCGAGAAGCCTCTGAGGACACCAGCTCCAGGGCCCCGAGCCAGGATT CCTTGGACGGCAAGCCCCTTCAGGAACAAATGGACGATCTGTTACTGCGGTGCTTCCTGCATGCCCTGAA GAGTCGAGTCAAGAAAGCTGACCTCCCGTTGCTCACCAGCACTCTCCTTGGCAGTCACATGTTCTCCTGC TGTCCTGAAGGACAGCAACTGGATATAAAGAAGTCAAGCTATAAAAAGCTCTCTAAGTTCCTGCAGCACA TGCAGCAGGAGCAGATTGTCCAGGTCAAGGAGCTGAGCAAAGGGGTGGAGAGCATTGTGGCTGTGGACTG GAGGCACCCGAGGATCACGTCTTTCGTCATCCCCGAGCCTTCCCTGACTTCCCAGACTGTCCAGGAAGTT AGCAGGGAACAGCCCTATCTCCCTCCAGATATAAAATCCCTCTACTGTGTCCCAGCCAACATGACCCAGC TCTTCCTGGAGTCTGGCCACAAGAAGGGGAGCACCCTTGAGGGCAGTGAGGTCCGAAAGATCATCACGGA CTATGCCAAGAGAAACAGACTGGTGGATGCAGACAACAGAAATCTGGTGAAGTTGGATCCTATCTTATGT GACTGCATCTTAGAGAAAAATGAGCAGCACTTGGTCACGAAGCTCCCATGGGACTGTCTCCTGACCAGGT GTTTGAAAAATATGCAGCCTGCCTACCAAGTAACATTTCCTGGCCAGGAACCCATTCTAAAGAAAGGGAA ACTCTGTCCAATTGATATCACCCTTGCACTGAAGACCTATAATAAAAAGGTGACTGTGGTCCGGAACTTG GAGACCTATGGTCTAGATCCGTGCTCAGTGGCCGCCATTCTCCAGCAGCGATGTCAGGCAAGCACCATCG TCTCTCCTGCCCCTGGGGCCAAAGACAGTCTGCAGGTGCAGGTCCAGGGAAACCAGATCCACCACCTTGG CCAGCTACTGCTTGAAGAGTATCGGCTCCCTGGAAAATACATCCAAGGCCTAGAAAAGGCCCCCAAACCT GGCAAGAAGTGACAGACTCATTTTATGCAGTGGACCCCATGGGAACCCAGGTTCTGAGCTGGTAACTGAT ACGAGCAGTTTTGGCTTTTGCAAATAAAAACTGTAATTCTTTACTGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4935223 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4935223 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14155 same embryo
	EMAGE:14153 same embryo
	EMAGE:14151 same embryo
	EMAGE:14150 same embryo
	EMAGE:14152 same embryo
	EurExpress:euxassay_001856 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source