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EMAGE:14155

Rsph9 radial spoke head 9 homolog (Chlamydomonas) ( MGI:1922814)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14155 EMAGE:14155 EMAGE:14155 EMAGE:14155 EMAGE:14155
"Pseudo-wholemount" of euxassay_001833. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001833_01 euxassay_001833_02 euxassay_001833_03 euxassay_001833_04
EMAGE:14155 EMAGE:14155 EMAGE:14155 EMAGE:14155 EMAGE:14155
euxassay_001833_05 euxassay_001833_06 euxassay_001833_07 euxassay_001833_08 euxassay_001833_09
EMAGE:14155 EMAGE:14155 EMAGE:14155 EMAGE:14155 EMAGE:14155
euxassay_001833_10 euxassay_001833_11 euxassay_001833_12 euxassay_001833_13 euxassay_001833_14
EMAGE:14155 EMAGE:14155 EMAGE:14155 EMAGE:14155 EMAGE:14155
euxassay_001833_15 euxassay_001833_16 euxassay_001833_17 euxassay_001833_18 euxassay_001833_19
EMAGE:14155 EMAGE:14155 EMAGE:14155
euxassay_001833_20 euxassay_001833_21 euxassay_001833_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14155Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14155_wholemount_strong.wlz
14155_wholemount_moderate.wlz
14155_wholemount_weak.wlz
14155_wholemount_possible.wlz
14155_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14155_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 06 moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
4th ventricle lateral recess
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4417
Entity Detected:Rsph9, radial spoke head 9 homolog (Chlamydomonas) ( MGI:1922814)
Sequence:sense strand is shown

>T4417
GCTTGGAGGAGAACGGCTGGCCTGATGGACGCCGACAGCCTCTTGTTGTCTCTGGAGTTGGCGTCTGGCA
GTGGGCAGGGACTCAGCCCTGACCGTCGGGCCTCACTGCTCACGTCCCTTATGCTGGTGAAGCGCGATTA
CCGCTTCGCGAGGGTCCTTTTCTGGGGCCGCATCCTTGGCCTTGTGGCGGATTACTACATCGCACAGGGC
TTGAGTGAGGACCAGCTCGCACCACGCAAGACGCTCTACAGCCTGAACTGCACAGAGTGGAGTCTCTTGC
CCCCTGCCACAGAGGAGATGGCCATGCAGATATCTGTGGTGAGTGGCCGTTTCATGGGCGACCCTTCACA
CGAGTATGAACACACAGAGTTGCAGAAGGTTAACGAAGGAGAGAAGGTCTTTGATGAAGAAGTGGTGGTC
CAGATCAAGGAAGAGACTCGCTTGGTGTCCATCATTGACCAGATTGACAAGGCTGTAGCTATCATCCCTC
GAGGTGCCCTCTTCAAGACCCCTTTTGGAGTCACCCATGTCAATCGGACCTTTGAAGGCCTGCCCCTGTC
CGAGGTCAGGAAGCTTAGCTCGTACTTCCACTTCAGGGAGGCTATTGATCTGAAGAACAAGACCTTGCTG
GAGAAGTCGGACTTGGAGCCCTCGCTGGATTTCCTGGACTCCCTGGAATATGACATCCCCAGAGGGTCTT
GGAGCATCCAGATGGAAAGGGGCAACGCACTGGTGGTGCTGCGCAGCCTGCTCTGGCCAGGCCTCACCTT
CTACCACGCTCCTCGCACCAAGAACTATGGCTACATCTACGTAGGCACAGGAGAGAAGAACATGGACTTG
CCCTTCATGCTGTAGGAAGGCAGGCTGGATATTTATGTGGGGTCGAAACACTCTTTCATAAAGTTCAACT
GTTGGGCCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4922914 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4922914 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14153 same embryo
 EMAGE:14151 same embryo
 EMAGE:14150 same embryo
 EMAGE:14152 same embryo
 EMAGE:14154 same embryo
 EurExpress:euxassay_001833 same experiment
 MGI:4827824 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS