EMAGE mouse gene expression spatial database

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EMAGE:14152

Plbd1 phospholipase B domain containing 1 ( MGI:1914107)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001850. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001850_01	euxassay_001850_02	euxassay_001850_03	euxassay_001850_04

euxassay_001850_05	euxassay_001850_06	euxassay_001850_07	euxassay_001850_08	euxassay_001850_09

euxassay_001850_10	euxassay_001850_11	euxassay_001850_12	euxassay_001850_13	euxassay_001850_14

euxassay_001850_15	euxassay_001850_16	euxassay_001850_17	euxassay_001850_18	euxassay_001850_19

euxassay_001850_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14152_wholemount_strong.wlz

14152_wholemount_moderate.wlz

14152_wholemount_weak.wlz

14152_wholemount_possible.wlz

14152_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14152_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vibrissa

strong

regional

strong expression: see section 05 06 07 18 19

4th ventricle lateral recess

moderate

regional

moderate expression: see section 06 07 08 09 weak expression: see section 04 05 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4948
Entity Detected:	Plbd1, phospholipase B domain containing 1 ( MGI:1914107)
Sequence:	sense strand is shown >T4948 GAAGCGCCCAAGCAGGCAAGACTAATGTCAGAAGAAAGTTAAGATCGGCCCGGCCTCTGCAACGCAGCGC CCTGCTACCATCCTCAAGTGCGGCTCTTGCAACAGCTGCCACCGCCGGCGCCAGCGGGACCTAATCCAAC CAGGCCGAGGTCACAGCAGAGGAGCGATGTGCCACCGCAGCCCTGGCAGGAGCCTCAGGCCGCCATCGCC GCTGCTGCTGCTGCTGCCATTGCTGCTGCAGCCTCCCTGGGCGGCCGGTGCTGCCAGCCAGAGCGACCCA ACAGGAGTCCACTGTGCAACAGCATACTGGTCCCCTGAATCCAAGAAAGTGGAAATCAAGACGGTACTGG ACAAGAATGGGGACGCCTACGGCTACTACAATGACTCCATTAAAACCACAGGCTGGGGCATCCTGGAGAT CAGAGCTGGGTACGGCTCTCAGGTCCTGTCCAATGAGATCATCATGTTTCTGGCTGGCTACCTCGAGGGC TACCTCACTGCTCTACACATGTACGACCACTTCACAAACCTGTACCCACAGCTGATCAAGAATCCTTCCA TTGTGAAAAAAGTGCAAGACTTCATGGAGAAGCAAGAAATGTGGACTAGGCAAAACATCAAAGCGCAGAA GGATGACCCATTCTGGAGACACACCGGCTATGTGGTGACCCAGCTCGACGGGCTGTATCTTGGAGCTCAG AAAAGGGCTTCAGAAGAGAAAATAAAGCCCATGACAATGTTCCAAATTCAGTTCCTGAACGCCGTTGGTG ACCTGTTGGACCTTATTCCCTCCCTATCTCCCACAAAAAGCAGCAGCATGATGAAGTTTAAGATTTGGGA AATGGGCCACTGTTCCGCTCTTATTAAGGTCCTTCCTGGATTTGAAAACATCTATTTTGCCCACTCAAGC TGGTACACATATGCAGCCATGCTCAGGATCTATAAACATTGGGACTTCAACATCAAGGACAAATACACCC TCAGCAAACGGCTGTCTTTCAGCAGTTATCCAGGGTTCTTGGAGTCTCTGGATGACTTTTATATTCTCAG CAGTGGCCTGATATTGCTGCAGACCACCAACAGTGTGTACAATAAGACCCTGCTGAAGCAGGTGGTGCCC AAGACTCTCCTGGCCTGGCAAAGAGTCCGTGTGGCCAACATGATGGCCGAGGGTGGTAAGGAGTGGGCAC AGATATTTTCAAAGCACAACTCTGGTACCTACAACAACCAGTACATGGTCCTGGACCTGAAGAAGGTGAC CATCAATAGGAGCCTGGACAAAGGCACTCTCTATATTGTGGAGCAAATCCCTACATATGTAGAATACTCC GATCAAACCAATGTCCTAAGAAAAGGATACTGGGCTTCCTATAATATCCCTTTCCACAAAACAATCTACA ACTGGAGTGGCTACCCTCTGCTGGTTCACAAGCTGGGCTTGGACTACTCTTATGACTTAGCTCCACGTGC CAAAATCTTCAGGCGTGACCAAGGGAACGTGACGGACATGGCCTCCATGAAATATATCATGCGATACAAC AATTATAAAGAGGACCCCTACAGCAAGGGGGATCCCTGCAGTACCATCTGCTGTCGTGAGGACCTGAATG GAGCCAGTCCAAGCCCTGGAGGATGCTATGACACCAAGGTGGCAGACATCTTTCTTGCATCTCAGTATAA AGCCTATGCCATTAGTGGTCCCACGGTACAAGATGGACTCCCGCCTTTTAACTGGAATCGGTTCAATGAA ACGCTCCATCGGGGGATGCCAGAAGTCTTTGACTTTAATTTTGTTACCATGAAACCAATTTTGTCATAGG ACAAAAAGTGAGACATAGAGATGACAGAAAGCTGCGAATAGGACACCAAAGGCACTCTTAGCTGTATTTT CCCATCTGTACTCTTAATAAAGAATATTAAATTCCCAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5133074 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5133074 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14155 same embryo
	EMAGE:14153 same embryo
	EMAGE:14151 same embryo
	EMAGE:14150 same embryo
	EMAGE:14154 same embryo
	EurExpress:euxassay_001850 same experiment
	MGI:4827251 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source