EMAGE mouse gene expression spatial database

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EMAGE:14617

Ncoa1 nuclear receptor coactivator 1 ( MGI:1276523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005512. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005512_01	euxassay_005512_02	euxassay_005512_03	euxassay_005512_04

euxassay_005512_05	euxassay_005512_06	euxassay_005512_07	euxassay_005512_08	euxassay_005512_09

euxassay_005512_10	euxassay_005512_11	euxassay_005512_12	euxassay_005512_13	euxassay_005512_14

euxassay_005512_15	euxassay_005512_16	euxassay_005512_17	euxassay_005512_18	euxassay_005512_19

euxassay_005512_20	euxassay_005512_21	euxassay_005512_22	euxassay_005512_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14617_wholemount_strong.wlz

14617_wholemount_moderate.wlz

14617_wholemount_weak.wlz

14617_wholemount_possible.wlz

14617_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14617_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 03 04 05 06 16 17 18 19 20 21 22 23 weak expression: see section 01 02 07 08 09 10 11 15

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 15 16 17 19 20 21 22 23

dorsal grey horn

moderate

regional

moderate expression: see section 07 08 09 10 12 13 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 14 16 17 18 moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9590
Entity Detected:	Ncoa1, nuclear receptor coactivator 1 ( MGI:1276523)
Sequence:	sense strand is shown >T9590 CAGGTGTGAAGTTCATCAACATGAGTGGCCTTGGGGACAGTTCATCAGACCCTGCAAACCCAGACTCTCA TAAGAGAAAAGGATCGCCATGTGACACGCTGGCATCAAGTACTGAGAAGAGGCGCAGGGAGCAAGAAAAT AAGTATTTAGAAGAACTAGCTGAACTACTGTCTGCCAACATCAGTGACATTGACAGCTTGAGTGTAAAAC CAGATAAATGCAAGATTTTGAAGAAGACAGTAGATCAGATACAGCTAATGAAGAGAATGGAGCAAGAGAA ATCCACGACTGATGATGACGTGCAGAAGTCCGACATCTCCTCGAGCAGCCAAGGAGTGATAGAGAAGGAG TCGCTGGGCCCTCTTCTTCTGGAGGCTTTGGATGGATTTTTCTTTGTTGTGAACTGTGAAGGAAGAATTG TATTTGTATCAGAAAATGTGACCAGCTACTTGGGTTACAATCAGGAAGAATTAATGAATACCAGCGTCTA CAGCATACTGCATGTGGGAGATCATGCAGAATTTGTCAAGAATCTGCTACCAAAATCACTAGTAAATGGA GTTCCTTGGCCTCAAGAGGCAACACGCCGAAATAGCCATACATTTAACTGCAGGATGCTAATTCACCCTC CAGAGGACCCAGGTACTGAGAACCAAGAAGCTTGCCAGCGCTATGAAGTAATGCAGTGTTTCACAGTGTC ACAGCCAAAATCCATCCAAGAAGATGGTGAAGATTTCCAGTCATGTCTGATTTGTATTGCTCGACGATTA CCTCGGCCTCCAGCCATTACAGGTGTAGAATCCTTTATGACCAAGCAAGATACTACAGGCAAAATCATCT CTATTGACACTAGCTCCCTGAGGGCTGCTGGCAGGACTGGCTGGGAAGACCTAGTAAGGAAGTGCATCTA TGCTTTCTTCCAACCTCAGGGCAGAGAGCCATCTTACGCCCGGCAGCTGTTTCAAGAAGTGATGACTCGT GGCACTGCCTCCAGCCCATCCTATAGATTCATATTGAATGATGGGACAATGCTTAGCGCCCACACCAAGT GTAAACTTTGCTACCCTCAAAGTCCTGACATGCAGCCTTTCATCATGGGAATTCATATCATCGACAGGGA GCACAGTGGGCTTTCTCCTCAAGATGACAGTAATTCTGGAATGTCAATTCCCCGAATAAATCCCTCAGTC AATCCTGGTATCTCTCCAGCCCATGGTGTGACCCGTTCATCCACGTTGCCACCATCCAACAACAACATGG TCTCTGCCAGAGTAAACCGCCAACAGAGCTCAGACCTCAACAGCAGCAGCAGTCATACTAACTCTAGCAA CAACCAAGGGAACTTTGGATGTTCACCTGGAAATCAGATTGTAGCCAATGTTGCCTTAAACCAGGGACAG GCAGGTTCCCAGAGCAGCAATCCCTCTTTAAACCTCAATAATTCTCCTATGGAAGGTACAGGAATTGCCC TCTCACAGTTCATGTCTCCGAGGAGACAAGCTAATTCTGGCTTGGCAACAAGGGCCAGGATGTCAAACAA TTCATTTCCTCCAAATATTCCAACATTAAGCTCCCCAGTTGGCATTACTAGTGGTGCCTGTAATAATAAT AATCGATCCTATTCAAATATCCCAGTAACATCTTTACAAGGTATGAATGAAGGACCCAATAACTCTGTTG GCTTCTCTGCTGGGTCTCCAGTCCTTCGGCAGATGAGCTCACAGAATTCACCTAGCAGATTAAGTATGCA ACCAGCAAAGGCTGAGTCCAAAGACAGCAAAGAGATTGCATCCATTTTAAATGAAATGATTCAGTCCGAC AACAGCGACAACAGTGCTAACGAAGGCAAGCCTCTGGACTCAGGACTTCTGCATAACAATGACAGACTCT CAGAAGGAGACAGTAAATACTCTCAAACTAGTCACAAGCTAGTACAGCTATTGACTACGACCGCAGAGCA GCAGTTACGGCATGCCGACATAGACACAAGCTGCAAAGATGTACTGTCTTGCACTGGTACTTCCAGCTCT GCCTCCTCTAACCCCTCAGGAGGTACTTGTCCCTCTTCTCACAGCTCTCTGACTGAGCGGCATAAAATTC TGCACCGGCTTTTACAGGAGGGCAGCCCCTCAGACATCACCACTTTGTCTGTGGAACCCGAGAAGAAGGA CAGTGTGCCAGCCTCTACTGCTGTGTCAGTGTCTGGACAATCCCAGGGGAGTGCCAGCATAAAACTGGAA CTGGATGCTGCAAAGAAAAAAGAGTCAAAAGACCATCAGCTCCTACGCTACCTTTTAGACAAAGATGAGA AAGATTTAAGGTCCACTCCAAACCTGTGCCTGGATGACGTAAAGGTCAAAGTGGAGAAGAAAGAACAGAT GGATCCTTGTAACACAAACCCAACCCCAATGACCAAACCTGCTCCTGAGGAAGTTAAACTGGAGTCCCAG AGCCAGTTTACAGCTGACCTTGACCAGTTTGATCAGTTATTGCCCACCCTGGAGAAGGCAGCACAGTTGC CGAGCTTGTGCGAGACAGACAGGATGGATGGTGCGGTCACCGGTGTCAGCATCAAGGCGGAGGTCCTGCC AGCGTCACTCCAGCCCACCACTGCCAGAGCTGCCCCCAGGCTGAGCAGATTGCCTGAACTAGAATTAGAA GCGATCGATAACCAGTTTGGACAACCAGGAGCGGGGGATCAGATTCCATGGGCAAATAATACTTTGACAA CAATAAATCAGAATAAACCAGAAGACCAATGTATTAGCTCACAGCTGGATGAGCTCCTCTGTCCACCAAC AACAGTAGAAGGAAGAAACGATGAGAAGGCCCTTCTGGAACAACTGGTGTCTTTCCTCAGTGGCAAAGAT GAAACGGAGCTGGCTGAGCTAGACCGGGCACTGGGGATCGACAAGCTCGTCCAGGGAGGCGGATTAGATG TATTATCTGAGCGATTTCCACCACAGCAAGCAACACCACCTTTGATGATGGAAGACAGACCCACCCTCTA TTCCCAGCCATACTCATCTCCTTCTCCCACCGCTGGTCTCTCTGGCCCTTTCCAAGGCATGGTCCGGCAG AAGCCTTCACTGGGGGCTATGCCGGTTCAAGTAACACCTCCTCGAGGAACCTTTTCACCCAACATGGGCA TGCAGCCCCGGCAGACTCTCAATAGACCTCCAGCTGCACCCAACCAGCTTCGGCTGCAGCTGCAGCAGCG GCTACAGGGGCAGCAGCAGTTGATGCACCAAAACCGGCAAGCCATCTTGAACCAGTTTGCAGCAAATGCT CCTGTTGGCATGAACATGAGGTCAGGCATGCAGCAGCAGATCACACCTCAGCCACCCTTGAATGCTCAGA TGTTGGCCCAGCGCCAGCGGGAGTTGTACAGTCAGCAGCATCGACAGAGGCAAATTATACAGCAGCAGAG AGCCATGCTCATGAGGCACCAAAGTTTTGGGAACAACATCCCTCCTTCATCTGGACTCCCAGTTCAAATG GGGACTCCCCGTCTTCCTCAGGGTGCTCCCCAGCAGTTCCCCTACCCACCAAACTATGGTACAAACCCAG GAACCCCACCTGCCTCCACCAGCCCTTTCTCTCAACTGGCAGCAAACCCTGAGGCCTCCTTGGCCACCCG CAGCAGCATGGTGAACAGAGGCATGGCAGGGAACATGGGAGGACAGTTTGGCGCTGGCATCAGTCCTCAG ATGCAGCAGAATGTCTTCCAATATCCAGGACCAGGACTAGTTCCCCAAGGTGAGGCCACCTTTGCCCCAT CTCTAAGCCCTGGGAGCTCCATGGTGCCGATGCCAGTCCCTCCTCCTCAAAGCTCTCTGCTCCAGCAAAC TCCACCTACTTCTGGGTACCAGTCACCAGACATGAAGGCCTGGCAGCAAGGAACAATGGGAAACAACAAT GTGTTCAGTCAAGCTGTCCAGAGCCAGCCTGCACCTGCACAGCCAGGAGTGTACAACAACATGAGCATCA CCGTGTCCATGGCAGGTGGAAACGCAAACATTCAGAACATGAATCCAATGATGGGCCAGATGCAAATGAG CTCTCTGCAGATGCCAGGGATGAATACTGTGTGCTCTGAGCAGATGAATGATCCAGCACTGAGACACACA GGCCTCTACTGCAACCAGCTCTCGTCCACTGACCTTCTCAAAACAGACGCAGATGGAAACCAGGACAAGA AGACAGAAGAGTTCTTCTCTGTGGTGACTACAGACTAGAGGAGCTCTACAGGTGCAGCAGGTTCAGGTGT TTGCTGACGTCCAGTGTACAGTGAATCTGGTAGGCGGGGACCCTTACCTGAACCAGCCTGGTCCACTGGG AACTCAAAAGCCCACGTCAGGACCACAGACCCCCCAGGCCCAGCAGAAGAGCCTCCTTCAGCAGCTACTG ACTGAATAACCACTTTTAAAGGAATGTGAAATTTAAATAATAGACATGCAGAGTTATACAAATATATTAT ATATTTTTCTGAGATTTTTGATATCTCAATCTGCAGCCATTCTTCAGATCGTAGCATTTGGAGCAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30628581 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30628581 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14616 same embryo
	EMAGE:14618 same embryo
	EMAGE:14619 same embryo
	EMAGE:14620 same embryo
	EurExpress:euxassay_005512 same experiment
	MGI:4826622 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source