EMAGE mouse gene expression spatial database

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EMAGE:14618

Pnma2 paraneoplastic antigen MA2 ( MGI:2444129)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005514. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005514_01	euxassay_005514_02	euxassay_005514_03	euxassay_005514_04

euxassay_005514_05	euxassay_005514_06	euxassay_005514_07	euxassay_005514_08	euxassay_005514_09

euxassay_005514_10	euxassay_005514_11	euxassay_005514_12	euxassay_005514_13	euxassay_005514_14

euxassay_005514_15	euxassay_005514_16	euxassay_005514_17	euxassay_005514_18	euxassay_005514_19

euxassay_005514_20	euxassay_005514_21	euxassay_005514_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14618_wholemount_strong.wlz

14618_wholemount_moderate.wlz

14618_wholemount_weak.wlz

14618_wholemount_possible.wlz

14618_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14618_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 14 weak expression: see section 08 15

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 17 weak expression: see section 06 16

trigeminal v nerve

weak

regional

weak expression: see section 09 16

ventral grey horn

moderate

regional

moderate expression: see section 08 09 11 12 13

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 14

cervical ganglion

weak

regional

weak expression: see section 08 15

thoracic ganglion

weak

regional

weak expression: see section 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 13 14 15

not examined

regional

not examined expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9591
Entity Detected:	Pnma2, paraneoplastic antigen MA2 ( MGI:2444129)
Sequence:	sense strand is shown >T9591 ACTTGGCTCCTTGGTTCTTGAAAAACATTCTGGAACACATGAACAGGAGTCCTTGCCATCTCACTGTGTG ACTCCCCAGAGTTGGGTGCTAGAGTGGAAGCCACCTCCCCCATTGACTAGAGAGAGGCGAAGTGACAGAA ACCAGGAGATGGTGGGAAAAGCGATTGTTCCAGGTGCCTCCCGATGACAAGAGTCAGCACCACGCAGTTA CAACAACCAGCCCTCGGAAGCAGCAGCGACCCCACTGCAGAGCAACAGCCCACCCTACGTACGCTGCAGG ACCCGTCAGCCTTGCTGTGGGTCTGAAGACAGACAGGGAGTGTCTGGTTCTTGTGACTGGGTAAAGGGAC AGAGCATTTGGCCCAAGAGGCTTTGAGACCTCTTGGAGACCAAGTCCAGAAGCATATCCTGGGACAATGG CGGTGGCCCTGCTGGAGGAGTGGTGTAAGATCATGGGTGTAGACGTCCAGAAGTCACTGCTGGTGGTTGA CATTCCGGTGGACTGTGGTGAACCTGAGATCCAGACAGTCCTCCAGGAGGCTCTGAAGTGTGTCGGCAGC TACCGGCTACTAGGCAAGATATTCCAGAAGCAGGACAACACCAGTGTTGTCTTAGTGGAACTTATGGAGG ACACAGATATGTCTGTGGTCCCCAGTGAGGTACAGGGCAAGGGAGGTGTCTGGAAAGTGATCTTCAAGAC CCCTAATCAGGACACTGAGTTTCTCCAAAGACTGAACCTCTTTCTAGAAAAAGAAGGGCAGACGGTCGCA GGGATGTTCCGAGCCCTTAAGCATGAGGGAGTGTCTCCAGCCACCCCACCCTGCACGTCCCCTGAGTTAT TGGCCCATTTGACGGGGCAGGCAATGGTTCACGGCCAGAGGCCTCTGTTGCCTGTGAAGTACTGTAAGAT GAGAATATTCTCTGGGAGCACAGCAGCTGCCCCAGAGGAGGAGCCCTTTGAGGTCTGGTTGGAACAGGCC ACTGAGATAGCCAAGGAATGGCCCATCCCTGAAGCAGAAAAGAAAAGGTGGGTGGCAGAGAGCCTCCGTG GCCCTGCCCTGGACCTCATGCACATAGTACAGGCAGACAACCCTTCCATCAGTGTGGGGGAATGTCTGGA GGCCTTTAAGCAGGTGTTTGGGAGCACGGAGAGCCGTAGAACCTCACAGGTAAAATACCTAAGAACCTAT CAGCAAGAAGGGGAGAAAATCTCAGCCTACGTGCTCCGGCTGGAAACTCTGCTCCGGAGAGCTGTGGAAA AAAGGGCCATCCCCCGGAACATTGCTGACCAGGTACGCCTGGAGCAGGTCATGGCTGGTGCCAACCTCGG CAACGTTCTGTGGTGCAGGCTACAGGAGCTGAAAGATCAGGGCCCACTCCCCACCTTTTTGCAGCTGATG AAGGTGATACGTGAGGAAGAAGAAGAAGAAGACGCCTACTTTGAACAAGAGAGCAGGGAGGAGCCGGGGG AAAGGGAGGGCTCTGGCTGCTGGAACAATTCAAGAAACAACTGAGAGATATCCCCACTCCGTCCCTGATC TGGTGGGCGATGCTTGACAAGAGACGATGCTTGACAAGGCTAACCCCATTACAGTGGATTCTTCTTAAGT GCAGGACTGAAGCTAAACATTAGGACTGAAGGTTTTTGTCTTTTCTTTCTTTTCTTTTGTTTTGATAGGG TCTCACCTTATAGCCCAAGTTGGCCTTGAACTGACATTCTTCCTGCCTCAACCTCCCAAATTGAGAGTTC TGGGATTAGAGGAACATCCCACCACTAGTTCCCCATCTCGGTTTTTCTTTCTGAGAAAAAAAAAAAATAC AAGACTAAGTTCCAATACCTAAAACTTCTGAACTTTAGTTTCCTTTAAGTACACCAGGGTTGGCCAGACA GCTCGGTTGATCAAGTTACTTACCATTCTCTTCAGAGGGAGCAATAATGTGAACAGGAGATAGGAATTGG CCAGGCAGGGGCTGGAGGGATGCTCAGCCATTAAGCACATTTGCTATTCTTTTGGAGGAGTTCAGATCGC AGCACCCATGTAGTGGTTCACAATTCTCTATAACGGGGATCCAACACCTTCTTCTGGTCTCTTTGGGTAC TTTGCATATGTGGTACACATGCATACATGCAGACCATACACATAAAATAAAAACAAATAAATCTTTAAGA AGAAAAGAACCTGGCTAGGCAGTTTCCAGGCATTCCAAAAGTGTCCCTGGTCTTCTGGTGCATGAACCTC ACAAGGAGCTCTGATTTCCCATTGTCCCCTTCCTGAAGGTTACACTTGAGGGTGGGACCTTCAGTCAGAC ACTAATCCATTTGTGGATAGTGTTAGAATTAGGTCCATAGATTTTTCATGATAAAATGGCTTATATCTGT TCCCCAGTTTTAGCCTAATCTCCTATATGATTTTAGGGGTAGCTCATCAGAGGGGGAAATTTACAGTAGC CTTGATCAGGACTTCTCTGAGTCTCATTAAGAGAAAACCATTGCCTTCTGGTCAGAAAAATGGAATCTAC CTCTTGCCTAGGCCGAGACCTACCATTGCAAGCTAACCAAAACATAAGCGTTAATCAAGTCGGGCAGGTC CATCACCAGGCTAATGGACTGCCCATTAGGAAGGCAGATTCACCTAACTCTATGCTAATGAGATGGAAGG AATAATTATCCCTTTAATGAAGTAATGTCCTTTTCCTTCCCAGAGTCCCTGTTATCTGTACAGTCACTAC AGAACTACCATGAAAAGTAATTTTCACTTTTTCTTTTTCTTTTTGCTGGCCCAGGCCTAAGCGCACATTC TTTAGTACTTTGAGCTGTTTCACTCTCTCCATGTGGCTGCATGCTAACCCTCTACCCACTCAAAAGAAAG CGTGGCTTTCTCTCTTCTTTTTCTCTATATCCCTCTTCCAGGTATGTTTGATCCCCATAAGCCATAGGGT AGAAGGAGAGAACCAATTCCTGCAAGCTGTCATTTTACACACACACACATGTACAAATAATGTAATTTTT AAAACCACAACAAGTAGACCAATGTTTACTCATACCTTTGAAAAATGGACTGACCTTATTTAAAAAAAAA ATTATACAAAACACATATATCCTACTTAATGTGTGCAACAACCTACTCTTTATAGGGTGTGTGTGTGTGT GCGCTTGCATGCATGTATGTGTGTGTGGAGGAGGGGTGGGGCTTGTGGTACACTTCATAAAGGGAAAGAT GCAAAGGTCAACTAAGTTAAATGGCCTCAGCACCAAAATGCTCAGAAGTGAAACCAGAGCAGAGGCCCAG AACAAGACTTTAGGGATCCACCCTCTGTGCGTCGCTCAAATGTGTTTTCTTAGTTCTGTAGAGGACTGAT TTTAAGCACTAGTGTTTTAAACTCCTTACATCTCAGACACCCAGCAGGTAGTATCAAGTGTGCTAATGAC CAAGTCTCAGTATCTCCGTGTTCACCAAGCTTGCTTTTGTTTCCAAACTGACCCAGGACTGCAGAACAAA GCTCCCTGCTGGCAATGGTGAGCCTATTCCAAAAGCCCTGTAATTGTGCACAGACTTGAAAAGCCATAGC GCCTTGTGAGTATGTGTGCCAGGTCAAGAGTACTAGGGCACCATAAACAAACACTGCTTAGTTGCCTGCC AGGCAGAGCTCGGGCACAGTTAGTGTGCAGCCTGTGGTAGATCCCTGTGTGGTTAGCAAGCTGCTTGGTT TCTCTGAACATCCGTTTCCTTATCTAAAAATGGGCTGCCATTAGGATCAAGTGGCACAATGCTTTTTAAG GGCTTGGAGTAACGTTTGGAACTTAGAAGCCCCTCCATATACTGAGGATCTTTGTATTTTTGTGATAGTT GTCAGCTCAATGCATGTTCTCTTGATTGTTTCCATTGTATGGAAACAAGTCACAAAGTAACAGACTTACA TTCCAGTCGGGATGCTCCTGCAGTGAGGAAGAACTTTGGCTTGGAAGTAAGTGTGCACTAGCTGGCCGTC CACATTTGTGGTAAGTATTCCAGAAGTAGTTGGTGACTGTTAATCGACTTGACTTTGGAATGATGACCTG TTCTTCCGGGTTGTGGGTTAGTTTAACTGAAGAATAAGAAGTTCTAAGAAAGGTGGTTTGTTTGTTTATT TAAGTCATCATCTGTGATGGTTTATCTTTCGGAAATATATTTCTCAACACTGTCCAGCCACAGTGATGTC AGTGATGGGATTGTCCTTGACCACTCTAGCCTGACTCCACGTGGCATTATGTTCCTGGGAATAGCTTTGC CGTTGTACTTACCAACAGAATACAGAGTTAAATGTTACCACTGCGAAGTTACCAAATAAATCTGAGCTAG TGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30618233 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30618233 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14616 same embryo
	EMAGE:14617 same embryo
	EMAGE:14619 same embryo
	EMAGE:14620 same embryo
	EurExpress:euxassay_005514 same experiment
	MGI:4827299 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source