EMAGE mouse gene expression spatial database

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EMAGE:14620

Rab40c Rab40c, member RAS oncogene family ( MGI:2183454)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005527. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005527_01	euxassay_005527_02	euxassay_005527_03	euxassay_005527_04

euxassay_005527_05	euxassay_005527_06	euxassay_005527_07	euxassay_005527_08	euxassay_005527_09

euxassay_005527_10	euxassay_005527_11	euxassay_005527_12	euxassay_005527_13	euxassay_005527_14

euxassay_005527_15	euxassay_005527_16	euxassay_005527_17	euxassay_005527_18	euxassay_005527_19

euxassay_005527_20	euxassay_005527_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14620_wholemount_strong.wlz

14620_wholemount_moderate.wlz

14620_wholemount_weak.wlz

14620_wholemount_possible.wlz

14620_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14620_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5390
Entity Detected:	Rab40c, Rab40c, member RAS oncogene family ( MGI:2183454)
Sequence:	sense strand is shown >T5390 GCGGCGGGTGGCGGCGGCGGCAGCGGCGGCGGCGGCGGCTAGGAGGCTGAGGTCTGGCGGCGGTGCCGTG GGCCGCGGTGCGGCGGGCGGGAGGATGGGCACCCAGGGCAGTCCAGTGAAGAGCTACGATTACCTGCTCA AGTTCCTGCTGGTGGGCGACAGCGACGTGGGCAAAGGCGAGATCCTGGAGAGCCTTCAGGACGGCGCAGC TGAATCCCCGTACGCCTACAGCAACGGGATAGACTATAAGACTACCACCATCCTCCTGGATGGACGGCGT GTCAAGCTGGAGCTATGGGACACATCGGGCCAGGGAAGGTTCTGTACCATCTTCAGGTCCTACTCAAGGG GTGCACAGGGAATCCTTCTGGTGTACGACATCACCAACCGTTGGTCCTTTGATGGCATTGACCGGTGGAT CAAGGAGATCGATGAGCATGCACCTGGTGTTCCCCGGATCCTGGTTGGGAACCGGCTGCACCTGGCCTTC AAGCGGCAAGTTCCAACGGAGCAGGCCAGAGCCTATGCAGAGAAGAACTGCATGACCTTCTTTGAAGTCA GCCCCCTGTGCAACTTCAACGTCATTGAGTCCTTCACTGAGCTCTCCCGTATCGTGCTCATGCGGCATGG CATGGAGAAGATCTGGAGACCCAATCGAGTTTTCAGCCTGCAGGACCTCTGCTGCCGTGCCATTGTCTCC TGTACCCCTGTGCACCTCATCGACAAGCTCCCACTACCTGTCACCATCAAAAGCCACCTCAAGTCCTTTT CCATGGCCAACGGCATGAACGCTGTCATGATGCACGGCCGCTCCTACTCCCTGGCAAGTGGGGCCGGGGG CAGTGGCAGCAAGGGCAACAGCCTTAAGAGGTCTAAGTCCATCCGGCCCCCTCAGAGCCCACCTCAGAAC TGCTCTCGGAGCAACTGCAAGATCTCCTAGCGGGACACATGGGTTCCGCCTGTTCAGACTTGGGGCTTAC GCTGGAGCACTCCTGACCATGCGCTAGGCCCTGCTGTGTGCAGGAAGACTTGGCTACACAACCCTGCCTG GGTCTCTCCAGGCTTTCCTCCACATCTGAGCTAGTGGAGGGAGGGGCATGCAGGAGAGAACATGCTCCGC AAACCAGGCAGCAGGGCTGCTGGTGCTACGGGACCAGGGGTGAGCCAGTCCTGGACTCCAGACTGGAAGT GAGCCAGGCTCCCCTTTATTTATATGGAGAACATTTTACCTGTTTTGTACATTTTTAAAGGGCTGTCAGG GAAGCCTGGATGCCCCTCATAGCCACATCAGCCGTTGGGGAAGCTCAGGATGTCAGGGAAGGAGGGATGA AGCAGTGGTGGGGGGTTTCAGTGTTGAAGCCAGATCTTTAGATTTATAAATACCCTGCCTGGGTGTTGCT CAGAGGCCACTGGGGAACAGTGTATTTCAGATGCTTCTAGCAGCAGGCTTGGCTCCGGCTTCCATATCTG ACAAAGGTGATCAGTATGCTGGGCATAGGCAAGGTTTCAGCTCCTAAGACCTAGGAGAAGAGCCGTTGAT GCACCTGGGAAACCCCACTAATCCCTCCTATTGTGCCCTGGCCCTTTCAGTGATGTTTACTATACACATC TCCAGCCGGGGCTACAGAGCTTCTGGCGGGCTCCGTGCATCTGGGCATGGGCACAGGGCCTAGGACCCCA GTGTCTAGCAGCTTGTACTCATCAACCTCGGCTGTGTTTCATAGCTGCGACTTGCCTCTTGTGGCCCTTC TGCCTTTAAGTTCATGTCACTAGAATCTCTTTGACCTGTTTCTTAGTGACACTCTCTGGAACACCAAGCC CCTCCTTAGGGACTCACTGGCCTGAGCAACTTGTGAAGCCATGGACTGTTCCCCCACAATGCTCCTCCTC TCCCAGTCTGGGCTGGGCAGCCTTCTCCCCTGTGGGGATGCTGTCCCCAGTGGATGTCCTGTGTGACTGC TTCCCTGTCCTTGCCTGGTGACACAGCCCTCAGGCACGCTGGTGCTTAACTCTACCTCCTGCCCTCAGGG CCCTGGCCATAACAGGAGCCATGACCCCTGACTGGGGACCTCTAAGTGCACGTTTCCTAAGATCATCTTA CCATGGATGTTACTAGGGCACTGGTAGACGCTGCGCCAGCCTGCTGTTCTGGGAGATTGAGTCAATGCCT GTAGGGAGGGACGGGGCTATGGTTGTGGGAACTGCCTGATAAGCAGCAGCCTGACCTGGGAGCCTGACCC AGAGGCTGGGTACAATCAAGCATCACCTTTCCCCACCAGTCCTGTTGCTGTTGACAGAGACCTTTTCTCT GGAATCGCGATAGAATGGTATAGACTTTGAGGACCTGGATTGGCCTGCGTGCACACCATCAACACTACAC TTGCTGCTGTGGACTCCACTATTCCTGGTTCTTTGTTCTTACTTCCTTGTACAGTAAATGTACTTCAGCA GTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:5364768 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364768 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14616 same embryo
	EMAGE:14617 same embryo
	EMAGE:14618 same embryo
	EMAGE:14619 same embryo
	EurExpress:euxassay_005527 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source