EMAGE mouse gene expression spatial database

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EMAGE:14622

H2afz H2A histone family, member Z ( MGI:1888388)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005523_01	euxassay_005523_02	euxassay_005523_03	euxassay_005523_04	euxassay_005523_05

euxassay_005523_06	euxassay_005523_07	euxassay_005523_08	euxassay_005523_09	euxassay_005523_10

euxassay_005523_11	euxassay_005523_12	euxassay_005523_13	euxassay_005523_14	euxassay_005523_15

euxassay_005523_16	euxassay_005523_17	euxassay_005523_18	euxassay_005523_19	euxassay_005523_20

euxassay_005523_21	euxassay_005523_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14622_wholemount_strong.wlz

14622_wholemount_moderate.wlz

14622_wholemount_weak.wlz

14622_wholemount_possible.wlz

14622_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14622_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9616
Entity Detected:	H2afz, H2A histone family, member Z ( MGI:1888388)
Sequence:	sense strand is shown >T9616 GGCGCGAGGAAGGCGGGAGACGGCGCAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGGGATCTCG CTGAGCGTCCGCCTGGCCTCGTCTCTTCCTCGCTCGTCGGAGCTTCAGCACGGTCCGAGATGGCTGGCGG TAAGGCTGGAAAGGACTCCGGAAAGGCCAAGACAAAGGCGGTTTCCCGCTCGCAGCGAGCCGGCTTGCAG TTCCCTGTGGGCCGTATTCATCGACACCTGAAATCTAGGACAACCAGCCACGGACGTGTGGGCGCGACCG CCGCTGTGTACAGCGCAGCCATCCTGGAGTACCTCACCGCAGAGGTACTTGAGTTGGCAGGAAATGCGTC AAAAGACTTAAAGGTAAAGCGTATCACCCCTCGTCACTTGCAGCTTGCTATACGTGGAGATGAAGAATTG GATTCTCTGATCAAAGCTACCATTGCTGGTGGTGGTGTCATCCCACACATCCACAAATCGCTGATCGGGA AGAAAGGACAACAGAAGACTGTTTAAGGATGCCTGGATTCCTTATTATCTCAGGACTCTAAATATTCCTA ACAGCTGTCCAGTGTTGGTGATTCCAGTGGACTGTATCTCTGTGAAAAACACAATTTTGCCTTTTTGTAA TTCTATTTGAGCAAGTTGGAGGCTTAATTAGCCTTCCAACCAACCAAATTTCTGCATTCGAGTCTTAACC ATATTTAAGTGTTACTGTGGCTTCAAAGAAGCTATTGATTCTGAAGTAGTGGGTTTTGATTGAGTTGACT GTTTTTAAAAAACTGTTTGGATTTTAATTGTGATGCAGAAGTTATAGTAACAAGCATTTGGTTTTGTACA GACATTGTTTCCACTCTGGTGGATAAGCTCAATAAAGGTCATATCCCAAACTAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:30622912 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30622912 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14626 same embryo
	EMAGE:14624 same embryo
	EMAGE:14621 same embryo
	EMAGE:14625 same embryo
	EMAGE:14623 same embryo
	EurExpress:euxassay_005523 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source