EMAGE mouse gene expression spatial database

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EMAGE:14624

Brpf1 bromodomain and PHD finger containing, 1 ( MGI:1926033)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005506. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005506_01	euxassay_005506_02	euxassay_005506_03	euxassay_005506_04

euxassay_005506_05	euxassay_005506_06	euxassay_005506_07	euxassay_005506_08	euxassay_005506_09

euxassay_005506_10	euxassay_005506_11	euxassay_005506_12	euxassay_005506_13	euxassay_005506_14

euxassay_005506_15	euxassay_005506_16	euxassay_005506_17	euxassay_005506_18	euxassay_005506_19

euxassay_005506_20	euxassay_005506_21	euxassay_005506_22	euxassay_005506_23	euxassay_005506_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14624_wholemount_strong.wlz

14624_wholemount_moderate.wlz

14624_wholemount_weak.wlz

14624_wholemount_possible.wlz

14624_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14624_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5370
Entity Detected:	Brpf1, bromodomain and PHD finger containing, 1 ( MGI:1926033)
Sequence:	sense strand is shown >T5370 CTGCTGCTGCCGCCGCTGCCACAGCCTTTGCCGCCACGGTCTCGCCGCTGCTGTTCCTGTGCCGCCTCCA GGAGCCCCCCCATTGTCCCGGCGGCACCGGGTTCCCAGGCCAGAAGATCCAAAAGCTCGGGAAACGCGAA GATGTGACAGCATGGGGGTGGACTTTGACGTGAAGACCTTCTGCCACAATTTGCGGGCAACTAAGCCACC ATATGAGTGCCCTGTGGAGACTTGCCGCAAGGTTTACAAGAGTTACAGTGGTATCGAGTACCACCTGTAC CACTATGACCACGACAGCCCACCACCCCCACAGCAGACCCCACTGCGCAAGCACAAAAAGAAAGGGCGCC AGTCACGACCAGCCAACAAGCAGTCACCCAGCCCCTCTGAAGTCTCACAGTCACCAGGCCGAGAGGTGAT GAGCTATGCTCAGGCCCAGCGCATGGTAGAAGTGGACCTTCATGGCCGTGTCCACCGAATCAGCATCTTT GACAACTTGGATGTGGTGTCAGAGGATGAGGAGGCCCCTGAGGAGGCCCCTGAGAATGGCAGCAACAAGG AAAACACTGAGACACCTGCGGCTACACCTAAGTCAGGCAAGCATAAGAACAAGGAGAAACGAAAAGACTC TAACCACCACCATCACAGCGCTCCTGCCAGTGCTGCTCCCAAATTGCCTGAGGTGGTGTATCGTGAGCTA GAGCAAGATACCCCTGACGCACCACCCCGGCCCACTTCCTACTACCGGTACATCGAGAAATCTGCAGAGG AGCTGGATGAGGAGGTGGAGTATGACATGGATGAAGAGGACTATATCTGGCTGGATATCATGAATGAGCG GCGGAAGACTGAGGGTGTAAGTCCCATCCCACAAGAGATCTTTGAGTACTTAATGGACCGTTTGGAGAAG GAGTCGTACTTTGAGAGTCACAATAAAGGTGACCCCAATGCACTAGTGGATGAAGATGCCGTGTGCTGTA TCTGCAATGATGGCGAGTGCCAGAACAGCAATGTTATCCTCTTCTGTGACATGTGTAACTTGGCTGTGCA CCAGGAGTGCTACGGTGTCCCCTATATCCCTGAAGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCT TCTCGTGCAGTGGATTGTGCTCTGTGCCCCAATAAGGGTGGTGCCTTCAAGCAGACAGATGATGGCCGCT GGGCCCACGTGGTGTGTGCCTTGTGGATCCCTGAGGTTTGCTTTGCCAACACAGTCTTCCTAGAACCTAT TGACAGCATTGAGCACATCCCACCAGCTCGGTGGAAGCTCACCTGCTACATTTGTAAACAGCGGGGCTCT GGAGCCTGCATCCAGTGCCATAAGGCCAATTGCTACACAGCCTTCCATGTGACATGTGCCCAACAAGCTG GCCTTTACATGAAGATGGAACCTGTGCGGGAGACAGGTGCCAATGGTACCTCCTTTAGCGTCCGCAAGAC AGCCTACTGTGACATCCACACACCCCCAGGTTCTGCTCGTCGCCTGCCTGCCCTATCCCACAGTGAGGGT GAGGAAGAAGAGGATGAAGAAGAAGATGAGGGTAAAAGCTGGAGCTCAGAGAAGGTCAAGAAGGCCAAGG CCAAGTCTCGGATTAAGATGAAGAAAGCTCGGAAGATCTTGGCAGAGAAGAGGGCAGCAGCACCTGTGGT GTCCGTGCCCTGCATCCCGCCACACAGGCTCAGTAAGATCACCAACCGCCTGACCATCCAGAGGAAGAGC CAGTTCATGCAGAGGCTGCACAGCTACTGGACTCTGAAACGACAATCACGGAATGGGGTCCCACTACTCA GGCGCCTACAAACACACCTTCAGTCTCAGAGGAACTGTGAACAAGTTGGGAGAGATTCTGATGATAAAAA CTGGGCCCTCAAAGAACAGCTCAAGTCCTGGCAGAGACTGCGGCATGACCTGGAGCGAGCTCGGCTGCTG GTGGAGCTGATCCGCAAGCGAGAGAAACTAAAAAGGGAGACGATCAAGATCCAGCAGATTGCCATGGAGA TGCAGCTGACCCCTTTCCTCATCCTCCTCCGAAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAA CATCTTCAGCGAGCCGGTCCCTCTGTCTGAGGTACCCGACTACCTAGACCACATCAAAAAGCCCATGGAC TTTTTCACCATGAAGCAGAACTTGGAGGCTTACCGCTACTTGAACTTTGATGATTTTGAGGAGGACTTCA ACCTCATTGTCAGCAACTGCCTAAAGTATAATGCCAAGGACACCATCTTCTACAGGGCAGCAGTGCGACT CCGTGAGCAGGGTGGTGCTGTGCTCCGTCAAGCCCGGCGACAGGCAGAAAAAATGGGCATTGACTTTGAG ACGGGCATGCATATCCCTCACAACCTGGCCGGAGATGAGGTCTCACACCACACTGAAGATGTAGAGGAAG AACGGCTGGTCCTGCTGGAGAACCAGAAACACCTGCCAGTAGAAGAACAGCTGAAGTTGTTGCTGGAGAG GCTGGATGAAGTCAATGCCAGCAAGCAGAGTGTGGGCCGCTCTCGGCGTGCAAAAATGATCAAGAAGGAG ATGACAGCATTGCGGCGGAAGCTTGCTCACCAGCGGGAGACTGGCCGGGATGGGCCTGAGCGTCATGGCC CCTCAGGTCGGGGCAATCTGACACCCCACCCAGCAGCCTGTGACAAAGATGGACAGACCGACAGTGCTGC AGAAGAGAGCAGCAGCCAGGAAACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAACCCCCGCACATGAG GTGGGCAGGAGAACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAGACAGCTGGACCGCCCAAGAGGCCGG GCCGGCCCCCAAAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTGTGGGGCCCCCTCA GCTCCCCATCATGGGCTCCCTACGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCAAGCTCAGAC AGCGACAGTGATAAGTCCACAGAAGATCCCCCAATGGACTTACCAGCCAATGGCTTCAGCAGTGGGAACC AGCCAGTGAAGAAGAGTTTCTTGGTGTACCGTAATGACTGCAACCTTCCCCGAAGCAGCTCAGACTCTGA GTCCAGCAGCAGCAGCAGCAGCAGTGCAGCCTCAGACCGGACCAGCACAACTCCCTCAAAACAAGGCAGG GGCAAGCCCTCTTTCTCTCGGGGCACATTCCCAGAAGACAGTAGTGAAGATACCTCAGGCACTGAGAATG AGGCCTACTCCGTGGGCACTGGCCGCGGCGTGGGCCACAGCAGTAAGTACCCTCACCCAAAGTCAGGGGT GCTGGGGACCCAGTTTCAAGGCCTTGCCAGCCCCCCAGCTGCTGATCCGCCCCCTCTCTCCCGTTCCTGT GAAGTGGTAAGAAAGAGTCTGGGTCGAGGAGCTGGCTGGCTGTCAGAGGATGAGGACTCCCCGTTGGATG CTCTGGACCTCGTGTGGGCCAAATGCCGAGGCTATCCATCATACCCAGCTCTGATCATTGATCCAAAGAT GCCCCGAGAAGGTATGTTCCACCATGGGGTTCCTATCCCTGTACCACCACTGGAGGTTCTAAAACTTGGG GAACAAATGACACAGGAAGCCCGAGAGCATCTCTACCTCGTTCTCTTCTTTGACAACAAACGAACCTGGC AGTGGCTGCCCCGGACTAAGCTTGTTCCTCTGGGTGTGAACCAGGATCTAGACAAAGAGAAGATGCTGGA GGGCCGCAAGTCCAACATCCGCAAGTCAGTGCAGATTGCTTACCACAGGGCTCTGCAGCACCGAAGCAAG GTGCAGGGTGAGCAGAGCAGCGAGACCAGCGATAGTGACTGATACTCTCAGAGCCCGGTGGTGCTGTGAT GGCTCTCCTTCCCTTTGCTCATTGTCCTGGAGTGATGCCGCCCTCTGCACTGATTAATTCCTGGTCTTGG GGCCAGTCTCAGGGGAAGTTGGGTAGGGGAGGTCCCTCCTGCCCTGAGTGCAGCTGGACTGTACAAAACA CTCCAAGGGCCCATGGCCACTCAGTGCAAGGAGAGGAGAGGTCTCAGAGGTCTAACGAGTTCAAAAACTC ATGGCATTGTAGGGCAGGGTGGTGGGCCACAGTAAGAATACTGTAAAAAACAGGCCCTACCACCCTTACC TACTCATGCCAGGGGAATTCATACCTGCCTAGAGGCCTGAGGCCCTTGCAGGTGGTAAGGTAAATCAGCA TCATCCCAGCCTAGCCGTGGGATTGATGTCTGTCTAGCCTGGAAGAGGGGCACTAGGTGACCCCTCCCCT GCTGTTGTAAATACTGTAATTATTGGAGAATTTAAATTATTCTCATTTGTAACTGCGTTTCCGGGTCGCG CCAGAGTCATTTGGTACTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363697 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363697 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14626 same embryo
	EMAGE:14621 same embryo
	EMAGE:14625 same embryo
	EMAGE:14623 same embryo
	EMAGE:14622 same embryo
	EurExpress:euxassay_005506 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source