EMAGE mouse gene expression spatial database

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EMAGE:14623

Gpr37 G protein-coupled receptor 37 ( MGI:1313297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005522. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005522_01	euxassay_005522_02	euxassay_005522_03	euxassay_005522_04

euxassay_005522_05	euxassay_005522_06	euxassay_005522_07	euxassay_005522_08	euxassay_005522_09

euxassay_005522_10	euxassay_005522_11	euxassay_005522_12	euxassay_005522_13	euxassay_005522_14

euxassay_005522_15	euxassay_005522_16	euxassay_005522_17	euxassay_005522_18	euxassay_005522_19

euxassay_005522_20	euxassay_005522_21	euxassay_005522_22	euxassay_005522_23	euxassay_005522_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14623_wholemount_strong.wlz

14623_wholemount_moderate.wlz

14623_wholemount_weak.wlz

14623_wholemount_possible.wlz

14623_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14623_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 07 08 11 12 15 16 17 18 19 20 21 22 23 24

medulla oblongata floor plate

moderate

regional

moderate expression: see section 10

metencephalon floor plate

moderate

regional

moderate expression: see section 11

midbrain floor plate

moderate

regional

moderate expression: see section 10

facial vii ganglion

weak

regional

weak expression: see section 03 04 19

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 07 08 17 18 19 20 21

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 07 16 17 18

facial vii nerve

moderate

regional

moderate expression: see section 04 18

trigeminal v nerve

moderate

regional

moderate expression: see section 04 05 06 19

spinal cord floor plate

moderate

regional

moderate expression: see section 09 10 11

neural retina

moderate

regional

moderate expression: see section 03 04 23 24

tongue muscle

weak

regional

weak expression: see section 12 13 14 15 16 17

testis

weak

regional

weak expression: see section 03 04 05 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5380
Entity Detected:	Gpr37, G protein-coupled receptor 37 ( MGI:1313297)
Sequence:	sense strand is shown >T5380 CGCTCCACTCCACGCCTTCTCAGCCCGTGCACTAGGCGCAGCCGCTGGCGCCATCCACTTGTCAACTTTG AACTGAAGGAGCAGCAACTTTGACAGTGGCCGCGGGTTTCGAATCTGGCTTCTCCTTGGCAACAGTCCCT TTGAAAGTCGTCGGAGTTAAACGAAGCAAGAGTTTCCTCTGTGCTCCGGTGGTGCCTTGTTTGCAAGGGT CCCCACCCCCCACCCCCCCGCCAGCCGAGAGTGGGATTGTGACAGCCAGTCCCCTAGTGGAGCGGACTTG TTTTTCTTTTTCTCTAGAGACCTCTACTAGCGACTGGATAAAGCAGTATGGAAAGGATGTAAATAGCAGA GCAACTGTTACCAAGAAGACAGCCACCACCCAAAGGCTGGGAGGAGCGCGGGGCTTCCTCCAGGCTGCGC CCAGTCGCAACATTAGGGAACAATCAGGTGGTGTTCGCAACTTTTCAAGGTCAGCCTCTTGGATCTGTTA TTTCCCTGGGAACCTTCGGGTGCACCGAGCTTGGATTAGATTTGGCTGGGAAATGCTCAGAACATGAAAC GTGGAAGGATTGTAGGGATGTGTTGATGACACCGTCCTTCCCCAAGCGCCCTGTTCCCTCCCAGCAATCT TCTGCTCCCATCCTCTACCCAGCGGTGAGTATGTGACATGTGCCTAACTCTCTGCACCAAGTTTGGCAGC AGGTGTCGGTGCTAAGCTAGAGCCAGGCTAGTAGGTGCGCCCTCGCCGAGCCATGCCAGCCCGGGGCGCG CCTCTCTCGCGCACATCGCGGCTGCTGCTCCTTCTGCTGTTTAAGGTTTCTGTCTCTGCTGCCCTGAGCT TTGTCCCTGAGCCCAGGAATGGAACTTGTCTGGGGGAGAGCTGTTCCCCACTGATCCAACGCCGCAGCAG GGATGCCCGTGGACCTGGGAATTCGGCAAAAGACGCTCTGCGAGTCCATGTGCCCAGAGAGAAACTGGAA GCAGAGGTGAGAGGGGCGACCTCTTGGGACCTCCCACCACCCAGGGGCGGTGACACAGGTGTGATTGAAG AAGCGGCGGCTGCTGGGCCCTTGGGACCTCCAACCAAGCCACCAAGTGCCTGGAGATGGAAAAGTGCCCA GGGTAAAGAACCATCCGGGCATTTGAGGAGAAGGGACCCCACGGATCCCCAACTCTTCTTTCCGACATCG GAGGGGGGAGAGATGAGTTCCAAGCGCGATGGGATTCCCCAGAGCAGGCAGGAGCACAGTGTGAAAACGG AACCCAGAGATCTTTTTTACTGGCCACGAAGAACTGGACAACTCCAGGATTCCCAACACAGGCCTTCAGC AGTACACGAAGGAAGGACCCTTGCTCCTCCAGGCAGGGCACTACCGCAAAACGGGTCTGCAGATGACTGG GTTCCTGACCAAGGGGGTCCACGCCGTGGAAACACCACGCGGCGCGTGAGATTGAAGAACCCCTTCTACC CATTGACCCAAGAATCCTACGGAGCCTATGCGGTCATGTGCTTATCTGTGGTGATCTTTGGGACCGGCAT TATTGGCAACTTGGCAGTGATGTGCATCGTGTGTCACAATTACTACATGAGGAGCATCTCCAATTCTCTC TTGGCCAACCTGGCTTTCTGGGACTTTCTCATCATCTTCTTCTGCCTCCCCCTGGTCATCTTCCATGAGT TGACTAAGAAGTGGCTTTTGGAAGATTTCTCCTGCAAGATCGTGCCCTATATCGAGGTGGCTTCTCTGGG TGTCACTACCTTCACCTTATGTGCACTGTGCATAGATCGCTTCCGGGCTGCCACCAACGTCCAGATGTAC TATGAAATGATTGAAAATTGCTCTTCCACCACTGCCAAACTTGCCGTTATCTGGGTTGGAGCTCTGCTTT TGGCCCTTCCGGAAGTTGTCCTCCGCCAGCTGAGCAAGGAAGATTTGGGCTTTAGTGGCCAGGCTCCTGC AGAACGGTGTGTCATAAAGATCTCTCCTGATTTACCGGACACAATCTATGTTTTGGCTCTCACCTATGAT GGTGCAAGGCTTTGGTGGTATTTTGGCTGCTACTTTTGTCTGCCCACACTTTTCACCATCACCTGCTCTC TAGTGACTGCTCGGAAGATCCGCAAAGCGGAGAAGGCCAGTACCCGTGGAAACAAGCGGCAGATCCATCT AGAGAGCCAGATGAACTGCACAGTGGTGGCTCTGACCATTTTATATGGGTTTTGCATCATCCCTGAGAAT ATCTGCAACATTGTCACTGCCTACATGGCCACGGGCGTCTCTCAGCAGACCATGGACCTGCTCAACATCA TCAGCCAGTTCCTTTTGTTTTTCAAGTCCTGTGTCACCCCCGTGCTCCTCTTCTGCCTCTGCAGACCCTT CAGCAGGGCCTTCATGGAGTGCTGCTGCTGCTGCTGTGAGGAGTGTATCCAGAAGTCTTCCACTGTGACC AGTGACGACAACGACAACGAGTACACCACAGAGCTGGAGCTGTCGCCCTTCAGTACTATCCGCAGGGAGA TGTCCACTTTTGCTTCTGTGGGGACTCATTGCTGAAGGAACGGCTTGGTTAGCTCTGATTTCTTCGATTT TCATATTCTGTGAAAATTTTTACTCCATATTTTTCCTTACAGGGGAACAATGCACACATTTTGAAAAGCA AGTAGAGGAAGACATATTAACTACTCTAGAACTGATTTTGCAAATTAATATTTGTGCTCTGGGAAAAAAT GTTTCTATTTAGTTATGTAAGAAGAAGGAGGCCAACAGTCTCAGATCATTTTTTTCTGCTATGGTGCTAA TATTTTATTCAAAAGTTATTGCACCTTAACAATATTAATTGTTTGATTTCTTTCTTTCTTTTAAAAACAT AATCGTTGTATATTGAAGGCAGGCCCGGGATTTTGCTAGTTATTTTGTGTTTGAGTTGTGATTGGAAGTA CATTGTATTTGAAATGGTTTGTTTTTTCCAGCCATGCAACTTAGCACCAAATAAACTTCTCATCCTTCTT TAATATGACTGTCAATCTACTTTTAACCAAGACTCAATAAATTTTTTAATTGCCTTAAATAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364562 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364562 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14626 same embryo
	EMAGE:14624 same embryo
	EMAGE:14621 same embryo
	EMAGE:14625 same embryo
	EMAGE:14622 same embryo
	EurExpress:euxassay_005522 same experiment
	MGI:4825188 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source