EMAGE mouse gene expression spatial database

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EMAGE:14625

Trappc9 trafficking protein particle complex 9 ( MGI:1923760)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005517. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005517_01	euxassay_005517_02	euxassay_005517_03	euxassay_005517_04

euxassay_005517_05	euxassay_005517_06	euxassay_005517_07	euxassay_005517_08	euxassay_005517_09

euxassay_005517_10	euxassay_005517_11	euxassay_005517_12	euxassay_005517_13	euxassay_005517_14

euxassay_005517_15	euxassay_005517_16	euxassay_005517_17	euxassay_005517_18	euxassay_005517_19

euxassay_005517_20	euxassay_005517_21	euxassay_005517_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14625_wholemount_strong.wlz

14625_wholemount_moderate.wlz

14625_wholemount_weak.wlz

14625_wholemount_possible.wlz

14625_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14625_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5388
Entity Detected:	Trappc9, trafficking protein particle complex 9 ( MGI:1923760)
Sequence:	sense strand is shown >T5388 CTTTCTTTCTTTCCTTTGTTTTCTATCATACCGCCCTGTGAGTATGTTGTTGGGGCTGAAAAGACACTAC AAGAAGCGGTGCCAAGGACGCATGCGGAAACACGTTGGGGACTTGTGCCTCCAGGCTGGGATGCTGCAGG ACGCCCTGGTACACTACCACATGTCCGTGGAGCTCCTGCGCTCAGTGAATGACTTCCTGTGGTTAGGGGC TGCCCTGGAGGGGTTGTGCTCGGCTTCAGTCATTTATCATTATCCCGGAGGAACTGGTGGCAAGACTGGG GCTCGGAGACTCCAAGGCAGCTCACTTCCCTCAGAAGCAGCCAACAGACACCGGCCAGGGGCGCAAGAAG TTCTCATTGACCCAGGTGCTCTCACCACCAACGGCATAAATCCTGACACCAGCACTGAGATCGGAAGGGC CAAGAATTGCCTCAGCCCCGAGGACATCATTGACAAATACAAAGAGGCCATCTCCTATTACAGCAAGTAC AAGAACGCCGGAGTGATTGAGCTGGAAGCCTGTGTCAAGGCCGTGCGCGTCCTAGCGATTCAGAAGCGTG GCATGGAGGCTTCGGAGTTTCTTCAGAATGCTGTGTACATCAATCTCCGGCAGCTTTCGGAAGAAGAGAA AATCCAGCGCTACAGCATCCTGTCTGAGCTCTACGAGCTGATTGGCTTCCACCGCAAGTCAGCATTCTTC AAGCGAGTGGCTGCCATGCAGTGTGTGGCGCCAAGCATTGCAGAACCTGGCTGGAGGGCCTGTTACAAGC TCCTTTTGGAGACGCTGCCTGGCTACAGTCTGTCGCTAGATCCCAAGGACTTCAGCAAAGGCACACACCG AGGCTGGGCTGCCGTTCAGATGCGTCTGCTGCATGAGCTGGTCTATGCCTCCCGCAGGATGGGGAACCCC GCACTGTCCGTGCGGCACCTCTCCTTCCTCTTGCAGACCATGCTGGACTTCCTGTCTGACCAGGAAAAGA AAGATGTGACTCAAAGCCTGGAGAACTACACTGCCAAGTGTCCCGGGACGATGGAGCCCATCACCCTCCC AGATGGTCTCACACTGCCTCCCGTGCCGTTTACCAAGTTGCCCATTGTCAGGTGCGTGAAGCTGCTGAGT CTCCCCACCAGCCTGCGACCCCACAAGGTGAAAAGCTTGCTGGGCCAGAGCATGTCCACCAAGAGCCCCT TCATCTATTCGCCAATCATCGCACATAACCGTGGAGAAGAACGCAACAAAAAAATAGATTTCCAGTGGGT TCAAGGAGATGTGTGTGAAGTTCAGCTGATGGTGTACAACCCAATGCCGTTTGAGCTCCGCGTGGAAAAC ATGGGGCTTCTCACCAGCGGAGTGGAGTTTGAGTCTCTTCCTGCAGCGCTTTCCCTCCCTGCGGAATCTG GTTTGTACCCGGTGACGCTGGTCGGAGTCCCACAGACTACAGGAATGATCACTGTGAATGGTTACCATAC CACAGTCTTTGGGGTCTTCAGCGACTGCCTTCTGGACAACCTTCCGGGACTGAAGACGGGCGGCTCCACC GTGGAAGTCATCCCTGCCTTGCCAAGACTGCAGATCAGCACATCGCTGCCCAGGTCCGCACGCTCACTGC AGCCTTCTGCCGGCGATGAGATCGCCACCAACGTATCTGTTCAGCTGTACAACGGAGAAACCCAGCAGCT GGCTGTCACCTTGGAGAACATCGGACTGGAGCCTCTGGAGCAGCTGGAGGTCACCTCAAAGCTTCTCACC ACCAAGGAAAAGTTGTATGGCGACTTCTTGAGCTGGAAACTGGAAGAGACGCTGGCCCAGTTTCCCCTGC AGCCCGGGAAGGTGGCTACTTTCACCATCAACATCAAGGCAAAGCTGGATTTCTCTTGCCAGGAGAATCT TCTCCAGGACCTGAGTGATGATGGAATCAGCGTGAGCGGCTTTCCCCTGTCCAGTCCTTTCCGCCAGGTG GTGCGGCCCCGAGTGGAGAGCAGACCCACAAACCCTTCTGAGGGCAGCAAGACGGGGGACCTTGGCCACG TCAAGACTCTGGAAGCTGTCCTGAACTTCAAGTACTCTGGAGGCCCAGGCCACGTGGAAGGGTATTACAG GAACCTCTCCCTGGGACTACACGTGGAAGTCGAGCCTTCTGTGTTTTTTACCCGAGTCAGCACACTTCCA GCAACCAGCACCCGGCAGTGCCACTTGCTCCTTGATGTCTTCAACTCCACAGAGCACGAGCTGACAGTCT GCGCCAGGAACAACTCTGAACTTGTCCTGCATGCCAGTGAGTGCCAGCGAATGGCTATTCAAGTGGACAA GTTCAACTTTGAAAGTGTCCCAGAATCCCCCGGAGAGAAGGGACACTTTGCAAATCTGAAGCAGCTGGAA GAAGAGAGACAGGAGGCACGAGGCCTGGAGATCAGCAGCAAGCTGGACATCCGCTGGAGAATTCCTTCTC TGAAGCGCAGTGGTGAGGCGAGCGTGGAGGGCCTCCTGAACCAGCTCATCCTGGAACACCTGCAGCTGGC ACCTCTGCAGTGGGATGTGCTAGTGGATGGGCAGCCATGTGACTGTGAGGTGGCAGCTGCCTGCCAGGTC GGTGACCCCGTGCGCTTGGAGGTGCGGCTGACTAACAGGAGTCCCCGAAGTGTGGGGCCCTTTGCCCTCA CCGTGGTCCCTTTCCAGGACCACCAGAACGGTGTGCACAACTATGACCTGCATGATGTCATCTCCTTTGT GGGTTCCAGTACCTTCTACCTTGACACGGTACAACCATCAGGCCAATCCACCTGCCTTGGGGCCCTCCTC TTCCTCTACACAGGTGACTTCTTCCTCAACATCCGGTTCCACGAGGACTGCAAGAGCAAGGAGCTGCCAC CCTCCTGGGTTTGCTTACCCAGCGTGCATGTGAGGGCCCTCGGGGCACAGGCCTGAGCCCCTCATCCCGA AGGCCATGTCCTCCATATGCCATAGCCAGCACTCCTCCTCCCTCCCAGAAGTCACCCCAAGGCTCCCCCC TGCTTTGCTTCTGGCCTATTATGCTGAGCGACCTCATATCCAGTGCCAGCCTGGGCGGCCCTGGATGGCC AGCACTGCAGAGGAGCCTTTCTGATCTGCCATCTCCCCTTGTGTCTCCTGAGGTCTGGCTCACCCCTGCA AATGTCACTGGCAACCCAAGCAAGGAGGGAGACAGTGTGGGTGAGCAGGGTGAGCAGGCATCAGGGTGTT CCCTGCCTCTGTGTTCTGGTCTCCAAGTCTGTGTATTTATGTTGCCACATTCTCCAATAAAGGCCTGCCT TGCTTACCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364921 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364921 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14626 same embryo
	EMAGE:14624 same embryo
	EMAGE:14621 same embryo
	EMAGE:14623 same embryo
	EMAGE:14622 same embryo
	EurExpress:euxassay_005517 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source