EMAGE mouse gene expression spatial database

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EMAGE:14812

Mei4 meiosis-specific, MEI4 homolog (S. cerevisiae) ( MGI:1922283)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005755. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005755_01	euxassay_005755_02	euxassay_005755_03	euxassay_005755_04

euxassay_005755_05	euxassay_005755_06	euxassay_005755_07	euxassay_005755_08	euxassay_005755_09

euxassay_005755_10	euxassay_005755_11	euxassay_005755_12	euxassay_005755_13	euxassay_005755_14

euxassay_005755_15	euxassay_005755_16	euxassay_005755_17	euxassay_005755_18	euxassay_005755_19

euxassay_005755_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14812_wholemount_strong.wlz

14812_wholemount_moderate.wlz

14812_wholemount_weak.wlz

14812_wholemount_possible.wlz

14812_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14812_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5555
Entity Detected:	Mei4, meiosis-specific, MEI4 homolog (S. cerevisiae) ( MGI:1922283)
Sequence:	sense strand is shown >T5555 GGCGCCTACCTGCGGGCTGCGCTTGCATCAGCGTGCATGGCCGGGAATACTGGCCCCCGAGCTTCCCCCG CCGCCTGAGGGCGTCCTTCCTTCTCAGGAACCCCAGGCTTGTGTAGCATCTGCCTGCTCTCTGTACTCTC GGGCCCCGGGTCCCCTCAAGCAGCGCACGCTCTCAGGTGCTGTGGCTTGGGGTTCCTTGTCGCGCCCTTT GCGGAGGGACAAAAGCCAAGATGGATATTCAGCCATGGTATTTGAAAACTTCAAAGCTGGCACTGGCGTT GGCGATTATCCACTCAAAACCAGCAGACAGAAGCAGCAGAGAATATACAGAGTACCTGGCTTCCTTGGTG ACCCAGAAGGACTCCACATGGAAATCAAAACTTGAAGCCTTAGAAGCAGAAGTTCTACAATTACGTCAAA AACTTCTCCTGAGTAGGATTTCCTCAGGATTGTTTAAGAATGGACCGGATGTGCTCCCCACGTTGTCAGA TCAGGAACCTACAAGTTCTGAAAATACATTGACACTGATGGATGACTCTGGATGTGTTTTATCAAATGAG CAAAGAAACGAACCTGCAGAATTATCCCAGCATTTTGTGGAGAGCACCGATCCCCCACTCCTCCCCCTGC CTCTTGAGAAAAGGCCACGCACGACCCTGGAAAATCCCCTGTCTTCTCACATGCAGTTCTTTCAACATCT GCTGGAGCTCAAGAAATGGACAGAATCCAGTAGTCTGAAAGTATACTTAACCCACTTTGAAAAAGACTCT TCCACAGTTTCTGATTCAGTTTCACAGTTGTTAGATGCCCTGATCACATTTTACAGGAACCCCAAACTCC CCTTCTCAAGCTTTTGGACAGAGGCTGTTGGCACATTAGCCAGACTGGCAAGTGATTTTAATTTATCAAA TCATATTTTTAAAAGATGTTCTAAGAAGTTGGAAGAATTTGAAAAAACTCTACTCCAGGCTATTTTAGAG AATAACAGTATAAACCGGTTCCAGGTACAGCGTTATGTCTCTCAGAGCCTAGTAACCCTAGGAAGTTGCA GCTTGTTGAGGAAGTCTATTATATCGCTGCTACTATCAGAAGTGAACAGCTTTGTGGATGACCTGGGAGC CATCGATCAGGATCAAGGCATTTATGATGTGACACGCTATGAAAATATTTTCTCCCTGTTTTGGATTCTG GAACAAGTTCTTCAACAGGCACCGCAGGGAGATCGCACTGCACACATGGATCACAGCATCCCAGAAATGC AGACATTTCTTCAGAAGCATGATGAAGTCATCTTCCGACTTTCGGATGCCTTTCCTTTATTTGCGTTCTA CTTATGGAGACTCGGTGTTCTCTTGAACTCAGCAGAGATGGAAACTGTTAAGAATGAATCGTTGCCTTAG CGGTTTTCCACAAATCAGAAAATGGTGTGCTTCAGTATTTCTCAAAAAAAAAGTTTTTAAATGAATATTT TAAATTGATTGGCCAATCGAATCATTAATTCAATTTAGTGATAGGTCTTCTATATAAGAGCTGATGAACC ACTGTTCCATATTACAATCAGGCAATTTCAGTTGACAATGCTCCCTTATGCTCGTGGATTATATCTTATT TCACTCAACAGTCTAGCTCTAGTCTCTAAAACAACCCATTTCATAAAATACAATAACTTGTCAAAGATTT CCATCTGATGTAAATTTCATTACAAATTGTAATGAAATTAAACTTAAACTTCTGTCATTTTACACACTTA AATACAAGTAAATGGTAAAAAATAAAATGTATTACGTAGCTCTACTCAAATCATTTTTCTCTTAAGGATA GTGCTGTAGAAAAAGAAAAGGCTTTGGAATCCAGTGGCTTTTAATTTTACTTAATCAACATGTTTGTAAA GTATTTTTGAAGTGTTAATTAGTTCTTTAAAAATGTCCCTTTTTAAAACAATTTTTCTGTTGATTATAAA TGCTATTGTTCCTTTGATTGTTTAAATGTAGTAAAGTTGTGTATTAGTAAGTCATCAGGGATGTTTTTAT AAATTTTAATATCCTACAATAAATACACATTATACTTATTAAAAGTACTGGTTGAATTTTTTCATTGCAC AATTTCCTTTACTTCATTTCAGTTATTAAAATTGTAACAGACAAGCTTAATGAGCACATATTTTGCACTT TCCTATTGTGCAGTTTAAAGAAAATATAATGTAATTCTCTTTCCTTTGTTAATTATGTCTAATTCTGTTT TATAAGGTAATTATCATTTTCAGATGCCATTAGTACTAGAAAAATTATCCTTGCTAATTCTTTCTACAAT GTATATAAAATTATGCCATATTCTAGTATTATTTTTGGAGTTTGTTTTTTTTTTTTTTTAAACCAGCAGA TCTCTTCTCTCCTAGAAATGGCTGTATAAACAAGACCTGAAAATGACTATATCAGTGGACATAGTAACAT GGAAGAGACAAAATTTCATGGGCTCACACTCCTAGATAAAGAACTAAAGACAAGCCGGGCATGGTGGCAC ACGCCTTTGATCCCAGCACTCGGGAGACAGAGGCAGGCAGATTTCTGAGTTTGAGGCCAGTCTGGTCTAC AAAGTGAGTTCCAGGACAGCCAGGTCTACACAGAGAAACCCTGTCTGGAAAAACAAAAATAATAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6469435 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6469435 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14811 same embryo
	EMAGE:14809 same embryo
	EMAGE:14808 same embryo
	EMAGE:14810 same embryo
	EMAGE:14813 same embryo
	EurExpress:euxassay_005755 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source