Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17101

5033414D02Rik RIKEN cDNA 5033414D02 gene ( MGI:1915009)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17101 EMAGE:17101 EMAGE:17101 EMAGE:17101 EMAGE:17101
"Pseudo-wholemount" of euxassay_001488. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001488_01 euxassay_001488_02 euxassay_001488_03 euxassay_001488_04
EMAGE:17101 EMAGE:17101 EMAGE:17101
euxassay_001488_05 euxassay_001488_06 euxassay_001488_07

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17101Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17101_wholemount_strong.wlz
17101_wholemount_moderate.wlz
17101_wholemount_weak.wlz
17101_wholemount_possible.wlz
17101_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17101_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
homogeneousstrong expression: see section 02 03 04 05 06
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4485
Entity Detected:5033414D02Rik, RIKEN cDNA 5033414D02 gene ( MGI:1915009)
Sequence:sense strand is shown

>T4485
CTCTGCTGCGGTCACCGTCCCCGCACCGTGTCGCGTGAGATCTCCAGAGGGACACTCCTGTTTGCCTTCT
ATATTTAACCGTGTACCTGAGATTTCCCCCGACACATCCAAAGTCCAAGTCCAAGAGAGCAAGGCCCAGA
AGGAGGTCAAGATGGGGTTTATATTCTCGAAATCTATGAACGAAAACATGAAAAACCAACAGGAGTTCAT
GGTTACGCATGCTCGGCTTCAGCTGGAAAGGCATCTGACCATGCAGAATGAGATGAGAGAAAGGCAAATG
GCTATGCAGATCGCCTGGTCTCGGGAATTCCTCAAATATTTTGGAACGTTTTTTGGCATTGCAACCATCT
CTTTAGCAACTGGAGCACTAAAGCGAAAGAAGCCAGCCTTTCTCGTTCCTATAGTCCCGCTAAGCTTCAT
CTTCACCTACCAGTATGACCTGGGCTACGGAACTCTCCTACAGAGAATGAAAAGTGAAGCTGAGGACATA
TTGGAAACAGAAAAGACGAAGCTGGAGCTGCCAAAAGGACTGATCACCTTTGAAAGCCTTGAGAAAGCCA
GAAGGGAGCAGAGTAAACTCTTCTCAGACAAATGAAGTCACAGTTATCCTCAGAGCACATATCGTGTCTC
AAAAGCACAAAGTTGTTGGCTTGAATCATGGTGTTTACAGTTTTTTAAATGATCAAGATTTTGATATGAT
GCATTTTTATTTTAGAATATTAAAATTCAAAATGAATTTGTTGAACTATTTTAAAATAAAATTTCTAACA
CTCCAAAAAAAAAAAAAAAG
Notes:The probe template was PCR amplified from IMAGE:4951101 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4951101 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17105 same embryo
 EMAGE:17103 same embryo
 EMAGE:17102 same embryo
 EMAGE:17104 same embryo
 EMAGE:17100 same embryo
 EurExpress:euxassay_001488 same experiment
 MGI:4822756 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS