EMAGE mouse gene expression spatial database

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EMAGE:17105

Anapc4 anaphase promoting complex subunit 4 ( MGI:1098673)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001474_01	euxassay_001474_02	euxassay_001474_03	euxassay_001474_04	euxassay_001474_05

euxassay_001474_06	euxassay_001474_07	euxassay_001474_08	euxassay_001474_09	euxassay_001474_10

euxassay_001474_11	euxassay_001474_12	euxassay_001474_13	euxassay_001474_14	euxassay_001474_15

euxassay_001474_16	euxassay_001474_17	euxassay_001474_18	euxassay_001474_19	euxassay_001474_20

euxassay_001474_21	euxassay_001474_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17105_wholemount_strong.wlz

17105_wholemount_moderate.wlz

17105_wholemount_weak.wlz

17105_wholemount_possible.wlz

17105_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17105_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 16

thoracic ganglion

moderate

regional

moderate expression: see section 12

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4477
Entity Detected:	Anapc4, anaphase promoting complex subunit 4 ( MGI:1098673)
Sequence:	sense strand is shown >T4477 GGCCGCTGGCTAGGAGTAGGCCGAGTCTGCGACTCTGGCAGGTACCGTTGCTGGCAGGGCTTTGTGCCAG AGCTTTGTGGCCGGGTGCTCCGGCGGCCTTCTCGCGCACGTCGTTGCAGGGCCGCACCCATGCTGCGCTT TCCGACCTGTTTCCCATCCTTTCGGGTGGTGGGAGAGAAGCAGCTGCCACAGGAGATTATTTTCTTGGCC TGGTCCCCCAAGCGAGACCTCATTGCTTTGGCCAACACTACAGGCGAGGTTTTGCTGCATCGCCTTGCAA GTTTTCATCGAGTTTGGAGTTTCCCACCAAATGAAAGTACAGGAAAAGAAGTGACCTGCTTGGCCTGGAG ACCAGACGGCAAACTTTTGGCCTTTGCTCTTGCGGATACCAAGAAAATTATTTTGTGTGATGTAGAAAAG CCTGAAAGCTTACACTCCTTCTCTGTGGAGGCTCCGGTCTCTTGTATGCATTGGACAGAAGTGACTGTGG AAAGCAGTGTTTTAACATCGTTTTATAATGCTGAGGATGAGTCCAATCTTCTCTTGCCTAAGCTGCCCAC ATTGCCAAAAAATTACAACAGCACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAATTATCAAGCTC TTGGGAGATGTCAGGCTGAACATCCTTGTCCTTGGAGGAAGCTCTGGATTTATTGAGCTTTATGCTTATG GGATGTTCAAAATTGCCCGAGTAACAGGGATTGCCGGAACTTGTATTGCTCTGTGTTTATCAAGTGATTT GAAGTCATTATCAGTGGTCACAGAGGTTTCCAGCGGTGGCGAATCAGAAGTTTCATATTTTCAGCTTGAA ACCAATCTGCTGTATTCCTTCTTACCTGAAGTAACTCGTATGGCCAGGAAGTTTACTCACATTTCAGCTC TCTTACAGTATATAAATTTGTCACTGACATGTATGTGTGAAGCATGGGAAGAAATTCTGATGCAGATGGA CTCCCGTCTTACCAAGTTTGTACAGGAAAAACCTACAACCACGTCAGTGCAGGATGAGTTCATGCACTTG CTGCTGTGGGGGAAAGCCAGTGCTGAACTTCAGACTCTCCTGATGAACCAGTTAACAGTGAAGGGCTTAA AAAAGCTTGGCCAGTCTATAGAGTCATCATATTCCAGTATACAAAAACTGGTTATAAGTCACTTACAGAG TGGCTCTGAGTCTTTACTGTATCATTTGAGTGAACTTAAAGGAATGGCTTCATGGAAACAAAAATACGAA CCTCTAGGACTGGATGCTGCAGGCATTGAAGATGCTATTACTGCTGTGGGTTCTTTCATCCTCAAAGCAA ACGAACTTCTCCAAGTTATAGACAGTAGTATGAAAAACTTCAAGGCATTTTTTCGGTGGCTGTATGTAGC AATGCTGAGAATGACAGAGGACCATGTGCTTCCTGAGCTGAACAAGATGACTCAGAAGGATATCACATTT GTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATAATAGGAAAGGAAAATACTTCA ATGTTGAAAGAGTTGGTCAGTATTTGAAAGATGAAGACGACGACCTTGTGTCACCCCCTAACACAGAAGG AAACCAGTGGTATGACTTCCTCCAAAACAGCACCCACCTGAAAGAAAGTCCTTTGCTGTTTCCTTATTAC CCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAACGTTATTGATCAGTGTTTGCAAAAGCCAG CAGATGTAATTGGAAGATCGATGAATCAAGCAATTTGCATTCCATTATATAAAGATGCTAGGAGTATGGA CTGTGCACGAAGATTGTTAAAATTCCCATTTCTGTGGAATAATAAAACTTCGAATCTTCATTATCTTCTT TTTACTATTTTAGAAGATTCGGTTTATAAAATGTGCATCTTAAGGAGACATACTGATATTTCCCAATCTG TAAGTAATGGACTAATTGGTATTAAATTTGGGAGCTTCACATCTGCCTCAGCTGACAAGGTCAGAAGAAG CTCTTACAGTTGTCTAGACGCCCAGTTCTATGATGATGAGACTGTCACGGTCATCCTGAAGGACTCCATG GGACGTGAAGGGAGAGACAGGATTTTGGTTCAGTTGTCACTGTCCTTGGTGTATAACAGTGAAGATTCTG ACGAATATGAGTTCACTGGGAGTTACTCGACAAGGCTTGACGAGCAGGGCAGCATTATCCCCACACGCAC CATGCACTTTGAGAAACACTGGAGGCTGCTAGAAAGCATGAGAGCGCAGTATGTTGCTGGGAACGGCCTT CGAAAAGTGTCCTGTGTGTTAAGTTCAAACCTCCGGCATGTGAGGGTGTTTGAAATGGACATCGATGATG AGTGGGAGATTGACGAGTCTTCAGACGATGAGGAGGAGGCTGGCGGGAAGCCTGTGAAGATCAAGGAGGA AGTGTTGTCAGAGTCAGAGACAGAGGCTCACCAAGACGCTGCTGCCCTAGACCCGGACGTAGTCATCAAA GTGGAGAAACTTGACCCTGAGTTGGACTCGTGACCCAGCTTGACAGCATTGTGTTAGTTAATGTGGCAAA AAGACGTAGTGGACGGACTAAGAATGTATCGGTCACACTGAGTTGTCTGTGTATCAAAGAAATAAATGGT AAATTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4949079 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4949079 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17101 same embryo
	EMAGE:17103 same embryo
	EMAGE:17102 same embryo
	EMAGE:17104 same embryo
	EMAGE:17100 same embryo
	EurExpress:euxassay_001474 same experiment
	MGI:4823119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source