EMAGE mouse gene expression spatial database

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EMAGE:17102

Prkd2 protein kinase D2 ( MGI:2141917)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001496. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001496_01	euxassay_001496_02	euxassay_001496_03	euxassay_001496_04

euxassay_001496_05	euxassay_001496_06	euxassay_001496_07	euxassay_001496_08	euxassay_001496_09

euxassay_001496_10	euxassay_001496_11	euxassay_001496_12	euxassay_001496_13	euxassay_001496_14

euxassay_001496_15	euxassay_001496_16	euxassay_001496_17	euxassay_001496_18	euxassay_001496_19

euxassay_001496_20	euxassay_001496_21	euxassay_001496_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17102_wholemount_strong.wlz

17102_wholemount_moderate.wlz

17102_wholemount_weak.wlz

17102_wholemount_possible.wlz

17102_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17102_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 07 08 12 13

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 weak expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4486
Entity Detected:	Prkd2, protein kinase D2 ( MGI:2141917)
Sequence:	sense strand is shown >T4486 GCCGACTTAAACTTTGAAGGGGGAAAAATAGAGCTACCCGCGCTGTGCGACCCTCCCTTGTCCCCGACCC GCCCCGCCTTCGGATCCCGCCATCCTCGCGTCTCTCAACCTGACAGATTTCTCTCTCAAATGCCTGCGTT CTTACCACCACCGCAAGAGAGCTCTCGGATCTAGGTCCCAGGGTCCTTGGTGAGCTTTGGGCGGCAGATC TGGATCTGGCCCCCATGGCCGCCGCCCCCTCCCATCCCGCAGGACTCCCCGGCTCTCCGGGGCCCGGGTC TCCTCCGCCCCCCGGTGGCTTGGATCTCCAGTCGCCGCCACCTCTGCTGCCTCAGATCCCGGCTCCGGGT TCGGGGGTCTCCTTCCATATCCAGATCGGATTGACCCGAGAGTTCGTGCTGTTGCCGGCTGCCTCAGAGT TGGCTCATGTGAAGCAACTAGCCTGTTCTATCGTGGACCAGAAGTTCCCAGAGTGTGGCTTCTACGGCCT GTACGACAAGATCCTGCTCTTCAAACATGACCCAACATCAGCCAACCTCCTGCAGCTGGTGCGATCAGCT GCAGATATCCAGGAGGGTGACCTGGTGGAGGTGGTTCTGTCGGCCTCGGCCACCTTCGAGGATTTCCAGA TCCGACCGCATGCTCTCACTGTGCACTCGTACCGCGCTCCCGCCTTCTGTGACCACTGCGGGGAGATGCT CTTCGGCCTCGTGCGCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAATTACCACAAACGCTGCGCCTTC AGCATCCCGAACAACTGCAGTGGTGCTCGAAAGCGCCGCCTGTCATCCACGTCTCTGGCCAGTGGCCATT CTGTGCGCCTTGGCAGCTCCGAGTCCTTACCCTGCACCGCCGAAGAGCTGAGCCGTAGCACCACCGACCT CCTTCCTCGCCGACCACCCTCGTCGTCGTCCTCCTCCTCTTCCTCTTCCTTCTACACAGGCCGACCCATT GAGTTGGACAAGATGCTGATGTCCAAGGTCAAGGTGCCACACACCTTCCTTATACATAGCTACACACGGC CCACCGTTTGCCAGGCTTGCAAGAAACTGCTCAAGGGTCTATTCCGCCAGGGCCTGCAGTGCAAAGACTG CAAGTTTAACTGTCACAAACGCTGTGCTACCCGCGTCCCTAACGACTGCCTGGGGGAGGCGCTCATCAAT GGAGACGTGCCGATGGAGGAAGCCGCTGATTACAGTGAGGCTGATAAGAGCTCCATCTCAGATGAGTTGG AGGATTCTGGTGTCATCCCCGGCTCCCACTCAGAGAGTGCTCTCCATGCCAGTGAGGAAGAGGAAGGCGA GGGACACAAGGCCCAGAGCTCGCTGGGATACATCCCCCTGATGCGCGTGGTACAGTCTGTGCGACACACG ACCCGGAAATCCAGCACCACCCTGCGGGAGGGCTGGGTGGTCCATTACAGCAACAAGGACACACTGAGGA AACGGCACTACTGGCGTCTAGACTGTAAATGCATTACCCTCTTCCAGAACAACACGACCAACAGATACTA CAAGGAAATCCCGCTGTCTGAAATCCTTGCGGTGGAACCGGCTCAGAACTTCAGCCTCGTACCCCCGGGC ACCAACCCACACTGCTTCGAGATCATCACGGCCAATGTCACCTACTTTGTGGGCGAAACCCCTGGCGGGG CCCCGGGAGGGCCAAGTGGACAGGGAACAGAGGCCGTTCGGGGTTGGGAGACAGCCATCCGCCAGGCACT GATGCCCGTTATCCTCCAAGATGCGCCCAGTGCCCCGGGGCACACACCGCACAGACAAGCGTCTCTGAGT ATCTCCGTGTCCAACAGTCAGATCCAAGAGAATGTGGATATCGCCACTGTCTACCAGATCTTCCCAGATG AGGTGCTGGGCTCTGGGCAGTTTGGAGTGGTTTATGGAGGAAAACACAGGAAGACTGGCAGAGACGTTGC AGTAAAGGTCATTGACAAACTGCGCTTCCCCACCAAGCAGGAGAGCCAGCTCAGGAATGAAGTGGCCATT CTCCAGAGTCTACGGCACCCCGGGATTGTGAACCTCGAGTGCATGTTTGAGACTCCTGAGAAGGTATTCG TGGTGATGGAGAAACTGCACGGGGACATGTTGGAGATGATCCTCTCCAGTGAGAAGGGCCGGCTTCCTGA GCGTCTCACCAAGTTCCTTATCACGCAGATCCTGGTGGCGCTGAGACACCTGCACTTCAAGAACATCGTC CACTGTGACTTGAAACCAGAGAATGTGTTACTGGCATCAGCCGATCCGTTTCCTCAGGTGAAGCTCTGTG ACTTTGGCTTCGCTCGCATCATCGGCGAGAAGTCCTTCCGGCGCTCAGTGGTGGGCACGCCTGCCTACCT GGCACCCGAGGTACTGCTCAACCAGGGTTACAACCGCTCGCTGGACATGTGGTCCGTGGGTGTGATCATG TACGTGAGCCTTAGTGGCACGTTCCCCTTCAATGAGGACGAGGACATCAATGACCAGATACAGAACGCGG CCTTTATGTACCCGGCCAGCCCCTGGAGCCACATCTCATCTGGAGCCATCGACCTCATCAACAACCTGTT GCAGGTGAAGATGCGCAAGCGCTACAGCGTGGACAAGTCTCTCAGCCACCCATGGTTACAGGAGTACCAG ACGTGGCTTGACCTCCGAGAGCTAGAGGGAAAGATGGGCGAGCGATATATCACGCACGAGAGCGACGACG CACGCTGGGATCAGTTTGTGGCAGAGCGCCATGGGACTCCTGCAGAAGGGGACCTGGGTGGCGCCTGTCT GCCGCAGGACCACGAGATGCAGGGGCTGGCTGAGCGCATCAGCATCCTCTGAGGCCAGTGTTCCCCCTGG GCAGCTTGCTTTCTTTGGAGTCCTGCACATCATCCCAGAAATGAACTCTTGTAGAGGAAGCAGCTTCCTG CGCAGACTGGAGGGGCCAGGGAAATAGACTCTTCCCAGGGCCATGATCTTAGTCCCTGATGCCTCAACCT TGGAGCGATAAGGCTGGAGAGTTGACTCTGGGGGTAAAGGCGCTTGCCACCAAGCCTAACAACTTGAGTT TGATTCCTGGGATGAACCGGTTTGGAGAATTGACTCCTTCCGTTTGTCCCCTGACCACCATAAGTGCCCA CATAAACACAGGCAATAAAATGTATTTTTTCTTTTTATAACAAATGAAGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4950957 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4950957 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17105 same embryo
	EMAGE:17101 same embryo
	EMAGE:17103 same embryo
	EMAGE:17104 same embryo
	EMAGE:17100 same embryo
	EurExpress:euxassay_001496 same experiment
	MGI:4827425 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source