EMAGE mouse gene expression spatial database

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EMAGE:17123

Mgat2 mannoside acetylglucosaminyltransferase 2 ( MGI:2384966)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001533. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001533_01	euxassay_001533_02	euxassay_001533_03	euxassay_001533_04

euxassay_001533_05	euxassay_001533_06	euxassay_001533_07	euxassay_001533_08	euxassay_001533_09

euxassay_001533_10	euxassay_001533_11	euxassay_001533_12	euxassay_001533_13	euxassay_001533_14

euxassay_001533_15	euxassay_001533_16	euxassay_001533_17	euxassay_001533_18	euxassay_001533_19

euxassay_001533_20	euxassay_001533_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17123_wholemount_strong.wlz

17123_wholemount_moderate.wlz

17123_wholemount_weak.wlz

17123_wholemount_possible.wlz

17123_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17123_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 07 08 09 10

submandibular gland primordium

moderate

regional

moderate expression: see section 06 15

basioccipital bone

moderate

regional

moderate expression: see section 02 21

exoccipital bone

moderate

regional

moderate expression: see section 02 03 15 19 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4506
Entity Detected:	Mgat2, mannoside acetylglucosaminyltransferase 2 ( MGI:2384966)
Sequence:	sense strand is shown >T4506 GCGGTCGCTGCCGGAGCGGGGCGAGGCTGCGGCGTTCGGTGTCGCCGTTCCGCCGTGCGAGCAAGGATGA GCGCGCAGAGGACGCAGAGCCACGGGCAGCGCAGGTAAAGGAATCACGGTGTCGGCGACCGCGGCGGGGC TTCTTCCGGGACCCCCGGGTGCTGAATGGAGAGGACCGAGGCGACGTTGAGCCGCGGCTCGTAGCGGCGG GGCGGCTGCCGGAGCTGCAACTGCCAGAGAGGATGCGCGGAGCCCGGGCGGCGCGAGGCAGTTGAGAGCC TTCGGGCCCCAGGACGCCGGAGCCCGGGATGAGTTAGCGAGGGCCGCCGCGGGGGCCAGTTCCTAGGGCT GCAGGCCAAGGCGACGGCGCCGCCCGCCCGCCCCTTCCGTGCAGAGGCCGCCAGCTTCTTTTCCGCGCCC GCGCTCCGGGCCCTGGAGACCATGAGGTTCCGCATCTACAAACGGAAGGTGCTAATCCTGACGCTCGTGG TGGCCGCCTGCGGCTTCGTCCTCTGGAGTAGCAATGGGCGACAAAGGAAGAGCGACGCCCTTGGCCCGCC GCTGCTGGACGCGGAGCCCGTACGGGGTGCGGGCCACCTTGCTGTGTCCGTAGGCATCCGCAGGGTCTCA AACGAATCGGCCGCTCCTCTGGTTCCCGCGGTCCCGCGGCCGGAGGTGGACAACCTAACGCTGCGGTACC GGTCCCTGGTGTACCAGTTGAACTTCGATCAGATGCTGAGGAACGTCGGTAATGACGGCACCTGGAGCCC CGGGGAGCTGGTGCTGGTGGTCCAGGTGCATAACAGGCCGGAATACCTCAGGTTGCTGATAGACTCGCTT CGCAAAGCCCAGGGTATTCAGGAAGTCCTAGTCATCTTTAGCCATGACTTCTGGTCCGCAGAGATCAACA GTCTGATCTCCAGGGTGGATTTCTGCCCGGTTCTGCAAGTGTTCTTTCCGTTCAGCATTCAGCTGTACCC GAATGAGTTTCCGGGTAGTGACCCTAGAGATTGCCCCAGAGACCTAAAGAAGAATGCAGCTCTGAAGTTG GGGTGCATCAATGCCGAATACCCTGACTCCTTCGGCCATTACAGAGAGGCCAAGTTCTCACAAACCAAAC ACCATTGGTGGTGGAAGCTGCATTTTGTATGGGAAAGAGTCAAAGTTCTTCAAGATTACACTGGCCTTAT ACTCTTCCTGGAAGAGGACCACTACTTAGCCCCAGACTTTTACCATGTCTTCAAAAAGATGTGGAAACTG AAGCAGCAGGAGTGTCCTGGGTGTGACGTCCTCTCTCTAGGGACCTACACCACCATTCGGAGTTTCTATG GCATTGCTGACAAAGTAGATGTGAAAACTTGGAAATCGACAGAGCACAACATGGGGCTAGCCTTGACCCG AGATGCATATCAGAAGCTTATCGAATGCACGGACACTTTCTGTACTTACGATGATTATAACTGGGACTGG ACTCTTCAGTATTTGACTCTAGCTTGTCTTCCTAAAATCTGGAAAGTCTTAGTTCCTCAGGCTCCTAGGA TTTTTCATGCTGGAGACTGTGGTATGCATCACAAGAAAACATGTAGGCCATCCACCCAGAGTGCCCAAAT TGAGTCATTATTAAATAGCAACAAACAATACCTGTTTCCGGAAACTCTAGTTATCGGTGAGAAGTTTCCT ATGGCAGCCATTTCCCCACCTAGGAAAAATGGAGGGTGGGGAGATATTAGGGACCATGAACTCTGTAAAA GTTATAGAAGACTGCAGTGAGGAAAAAAAATATCACAATTGCAAAAGCAAAAGTGACAGTCTTCTATTTT TTATATTTGTCTCAATGGGATATACAATTGAATAAAGGTATAGGAACCGGCCTCTGCTTTAATACATAAA CTTCTTGTAAAAGGTGTCCAGATACATAGCAATCCTTTCCAGTTAGGACTGATTAAAATTTCAAACTGAA ATTTTCATTTTGGGACTGGGTTTTAAAATTCAGTCTATCTGCTTAAAAGTAATGATTAGATTATTGATAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4954047 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4954047 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17122 same embryo
	EMAGE:17126 same embryo
	EMAGE:17121 same embryo
	EMAGE:17125 same embryo
	EMAGE:17124 same embryo
	EurExpress:euxassay_001533 same experiment
	MGI:4826186 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source