EMAGE mouse gene expression spatial database

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EMAGE:17125

Poc5 POC5 centriolar protein homolog (Chlamydomonas) ( MGI:1914713)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001514. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001514_01	euxassay_001514_02	euxassay_001514_03	euxassay_001514_04

euxassay_001514_05	euxassay_001514_06	euxassay_001514_07	euxassay_001514_08	euxassay_001514_09

euxassay_001514_10	euxassay_001514_11	euxassay_001514_12	euxassay_001514_13	euxassay_001514_14

euxassay_001514_15	euxassay_001514_16	euxassay_001514_17	euxassay_001514_18	euxassay_001514_19

euxassay_001514_20	euxassay_001514_21	euxassay_001514_22	euxassay_001514_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17125_wholemount_strong.wlz

17125_wholemount_moderate.wlz

17125_wholemount_weak.wlz

17125_wholemount_possible.wlz

17125_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17125_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05

spinal cord

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 12

cervical ganglion

moderate

regional

moderate expression: see section 06 07 13 14

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 12

neural retina

weak

regional

weak expression: see section 02 03 04 05 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4510
Entity Detected:	Poc5, POC5 centriolar protein homolog (Chlamydomonas) ( MGI:1914713)
Sequence:	sense strand is shown >T4510 CCACGCGTCCGAGGGGAGCCGCTGTGCTTGCTTTGGTTGCGGCGGCGACGCCGTCTGTCGCAGCGAGTCA GGAGCCGGGGTTTCGCCGAGACGGTGGATCCCGGTGGCGGCGCCCAGGCCGGTTCCTCCCTGGCAGGGTT GGGAGCATCGTCCCTGGAGGTGCCAGAAAGAGAAGACACAAGTGATGCGAACTAAATGAGTAAATCCATA GGAGCCCAGGGATACTGACTCCTGCGTGCCCACAAACCCGATGCTCCAGAGGCAACCTCATGCCGGCACA GCTGGTACTGAACTTGCTGACCTTCCAGTCCCATTGTGCCACCGTCCTTGCTTCGTGTTTGCAGAATGTC ATCTGATGAGGACAAGTGTTCACTTCATGTGGCTCACAATGACTCTGACCGTTCTGTCTCCACAGACCTT CAGGAGGAGTATGAAGAGCTGCTTCGCTATGCTATTGTGAATCCAAATGTTGAATGCAGTGCTTCGCAGC CATCTCACCTTAGAGGAGAAGTGGCACCGGATAGATTCCCTACGCTAGCTGGAAATAACCCTGTAAGAGA CACTGAACTGGAAGTGGGAAAAGGATCCGATTTAAATATTTCAAGCCATTCAAAATCAGGGTCACCCAGG AAGCCATCTCACCCAGTCATGGACTTTTTCAGCTCACATGTTTTAGATGACTCTTCCTCGCCAGCCTCTC TTTCTACTCGGACAGATGCCCATGAAATAAATGGGGGGGAGCATCTCGTCTCTGATGAAAATTTTCAGAA GTTGGAGAATATACTTGATATCTGGAGTTCAGGTCTTAAGACGAACGTTTTGTCTGAGCTGAGTAAGTGG AGGCTGAACTTTATCGACTGGCACCGAATGGAGATGAAGAAGGAGAAGGAAAAGCATGCGGTGGTCGTGA AGCAGCTGTCAAACCAGATCTCGGACCTGAAGGAGCTGCAGAAAGCCTTTGAGATCTCCATCGGGAGAAA GGATGAGGTGATTTCCAACCTGTCTCGTGCCATCGGCAAGCAAAAGGAGAGGATAGAGTTAATGAAAAGC TTCTTCCGCTGGAGGATCGGCCATGTCAAATCCAGACAGGAAATTTATGAAGGTAAACTGGCTGACCAGT ACTTTCAGAGAACTTTGCTGAAGAAAGTCTGGAAGGGCTGGCGCTCTGTGGTACAGAGGCAGTGGAAAGA TGTGGTGGAACGTGCGTGCCAAGCGAGGGCTGAAGAAGTATGTGTGCAGATCTCCAATGATTATGAAGCC AGACTAGCTATGTTATCTGGAGCTTTGGAAAATGCAAAGGCTGAGATTCAAAAAATGCAGCAGGAAAAAG ACCATTTCGAAGACTCCATGAAGAAAGCATTCATGCGGGGAGTGTGTGCATTAAATCTGGAGGCCATGAC CATATTTCAGAACAAAAATGAGGCAGGGATAGACTTCACAAATAATAAAAAGGAAGAGAGTGGCCCTGGC GGTCCCGGAAGAGAGCCTTCTGCTCACTTGGATACTTCTTCGTCCACGATGCCGTCAGCAGTTCCGTCAC AGCTGCTGCCCTCTGGACCATTAGCAGCAGGAGTAGCCAGTGCCACTGCCTTTCCCTCCGCTGCTTCCCT GACCTCGGCCGGAGCTACATCGGCCTCTTCTCTTCACGTTCCCATCTCTATTCTCAGTGCAGGATCTGCA GCTGCTCCTGCCCCAGAGGAATCGTACGCACCAAGAGTCGTGACCGCAGCCCAGCAGAAAGCTGGGAAGA CAATTACAGCCCGGATCACAGGTCGGTCTGATTTTGGTTCCAAAGCTAGAATCAACAGCAGTTTGGCGAT AATGGGAGTCTCTCCTCCCATGGGCTCAGTCGTTGTGGAGAAACATCATCCAGTCACAGTGCAAACCATT CCTCAGGCAACTGCTGCCAAGTACCCACGGACCATCCATCCTGAAAGCAGCATCTCCGCTTCTAGGTCTC TCGGAGCCAGGCCGGGCCACGCCCAGTCTCTCTCAGTTCAGTCGATAAAAGTGGTTGACTAGAGGGGCTG TGTTCACACTCATGGCTATGAGTCCTCTGATAAGGACTTTCCTGGGAGCAGCAAGCTTCTTGGTTTTGAC TTTTCTGTACTGTCAGAGCATCATGGACATATCATGTGCATCTTCTCAGAATTACAAGAGACTTCCCCAT CCTTTGTAACTGTATGTATAGATTATTTTAATGTTATTTTTATTTAAACAATTAAGTAGAACTTTTTAAT TAAAACAAACAAAAAAACCTTGGTTCTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4954391 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4954391 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17122 same embryo
	EMAGE:17126 same embryo
	EMAGE:17121 same embryo
	EMAGE:17124 same embryo
	EMAGE:17123 same embryo
	EurExpress:euxassay_001514 same experiment
	MGI:4822603 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source