EMAGE mouse gene expression spatial database

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EMAGE:17124

Shisa4 shisa homolog 4 (Xenopus laevis) ( MGI:1924802)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001517_01	euxassay_001517_02	euxassay_001517_03	euxassay_001517_04	euxassay_001517_05

euxassay_001517_06	euxassay_001517_07	euxassay_001517_08	euxassay_001517_09	euxassay_001517_10

euxassay_001517_11	euxassay_001517_12	euxassay_001517_13	euxassay_001517_14	euxassay_001517_15

euxassay_001517_16	euxassay_001517_17	euxassay_001517_18	euxassay_001517_19	euxassay_001517_20

euxassay_001517_21	euxassay_001517_22	euxassay_001517_23	euxassay_001517_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17124_wholemount_strong.wlz

17124_wholemount_moderate.wlz

17124_wholemount_weak.wlz

17124_wholemount_possible.wlz

17124_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17124_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

tail dorsal root ganglion

weak

regional

weak expression: see section 04 05 06 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4482
Entity Detected:	Shisa4, shisa homolog 4 (Xenopus laevis) ( MGI:1924802)
Sequence:	sense strand is shown >T4482 GCGGGCTGGGCGGTGCTGCAGCTCCCGCCGGCCGGCTTCGTCCAGCTTTGTCCTGCGGCCCCTTTTCCAG GCCGTCCTGGCCGCGCCCAGCCCCCTCTCGCCATGCCGCCCGCCGGGCCCCGCGGGACCGCGCCGCTCGC AGCCGTCGTCCTGCTGGTGCTTGGGGCTCCTCTGGCGCTGGCGAGTGAGGACTGCTTGTGGTATCTGGAC CGGAATGGCTCCTGGCATCCGGGATTTGACTGCGAGTTCTTCACCTTCTGCTGCGGGACCTGCTACCAGC GGTACTGCTGCAGGGACCTGACCTTACTCATCACCGAGAGACAGCAGAAACACTGCCTGGCCTTCAGTCC CAAGACCATAGCAGGCATCGCCTCTGCTGTAATCCTCTTTGTTGCTGTGGTTGCCACCACCATCTGCTGC TTCCTCTGTTCCTGTTGTTACCTATACCGAAGGCGCCAGCAGCTCCAGAGCACGTTTGAAGGCCAGGAGA TTCCTATGACAGGCATCCCAATGCAGCCAGTATACCAATACCCCCCGGATCCCAAAGCTGGCCCTGCACC TCCACAGCCTGGTTTCATGTACCCACCTAGCGGCCCTGCTCCTCAGTATCCCCTCTACCCAGCTGGGCCC CCAATCTACAACCCTGCAGCTCCTCCGCCTTATATGCCACCACAGCCCTCTTATCCAGGAGCCTGAAGAG ACAGCTGTGGCTCTGCTGTCCCTTCTGTGATGTCGTTTTTTGGTTTTTTTTTTTTTTTTTTGGAGAGTCC CCGTCCTGTACCTGCATCTAGCCCTGAGGGTGGGCAAGGGTCTCCTAGTCATCAGGCCAGCCCCCAGTCA AACCAAGCCTTGGGCCCTGCTGAGGACAGAGCCCCAGAGAAGTGGAACGTGAGCTGAGCTGGAACCAGGC CGTGGATGAGGGGTGGGTAGGGAGGACTTGGGATTCATGAGCTATTTTTACTGGGAGACTGGGAGTAAGA TGAGAGCCTAGGCCACAGTCTCTGTTTTCAAGTCGTCCCTCTGTGCCCAGGACCCCAATCAGAAAGGTGG GACCCCTTCTATATGCCCAGTTTGGGCTGAGGTGGAGACAAGTGAGGGGGCTCTATCAACAGCTGGCCAC AGCCCTTCCCTCTGGCTGCCCAATTGGCTGGAGCTCAGGCCTCCTGGATTAAAGCTGTAATGCTGTAACT CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4950177 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4950177 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17122 same embryo
	EMAGE:17126 same embryo
	EMAGE:17121 same embryo
	EMAGE:17125 same embryo
	EMAGE:17123 same embryo
	EurExpress:euxassay_001517 same experiment
	MGI:4828058 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source