EMAGE mouse gene expression spatial database

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EMAGE:17258

Pkmyt1 protein kinase, membrane associated tyrosine/threonine 1 ( MGI:2137630)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001641. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001641_01	euxassay_001641_02	euxassay_001641_03	euxassay_001641_04

euxassay_001641_05	euxassay_001641_06	euxassay_001641_07	euxassay_001641_08	euxassay_001641_09

euxassay_001641_10	euxassay_001641_11	euxassay_001641_12	euxassay_001641_13	euxassay_001641_14

euxassay_001641_15	euxassay_001641_16	euxassay_001641_17	euxassay_001641_18	euxassay_001641_19

euxassay_001641_20	euxassay_001641_21	euxassay_001641_22	euxassay_001641_23	euxassay_001641_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17258_wholemount_strong.wlz

17258_wholemount_moderate.wlz

17258_wholemount_weak.wlz

17258_wholemount_possible.wlz

17258_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17258_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 13 14 15 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 11 19 20 21 22

vibrissa

moderate

regional

moderate expression: see section 05 06 08 09 10 21 22 23

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 12 17 18

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

strong

regional

strong expression: see section 11 12 13 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5673
Entity Detected:	Pkmyt1, protein kinase, membrane associated tyrosine/threonine 1 ( MGI:2137630)
Sequence:	sense strand is shown >T5673 CGCTGCTGCGCCACGCCCAGCCTCCCCAGGTACTTGTGGCCCACACGACCTTCATGTGGACAATACAACG TGTGTGTGGCAAAGAAGTGGCTCTCTTGTGGGCCACAGGACCAAGTGCTGTTTGAGTGGACACATTGCTT TGTGCTCGGATCCTTTAGCGTTGGCCATGACCATGCCCACCGAGGGCACCCCACCACCCCTAAGTGGTAC CCCCATCCCAGTTCCAGCTTACTTCCGACACGCAGAGCCTGGTTTCTCCCTCAAAAGGCCTGGGGGCCTC AGTCGGAGCCTCCCACCTCGGCCCCCTGCCAAGGGCTGCATCCCTGTCAGCCGTCTATTCCCCCCTCGCA CCCCAGGCTGGCACCAGCCCCAGCCCCGGAGGGTGTCTTTCCTGTGTGAGACCTCAGAGCCCCTGCAGAG TCCTGGGTATGACCCGAGCCGGCCCGAGTCCTTCTTTCAGCAGAACTTCCAGAGGCTCAGCCGCCTGGGT CATGGCTCATATGGAGAAGTCTTCAAGGTGCGCTCTAAGGAAGATGGGCGACTCTATGCTGTTAAGCGCT ACATGTCGCCATTCCGCGGCCCCAAAGACCGAACTCGTAAACTGGCTGAGGTAGGTGGCCATGAGAAAGT GGGGCAGCATCCACACTGCGTGAGACTGGAGCGGGCCTGGGAGGAGGGTGGCATCCTATACCTGCAGACA GAACTCTGCGGGCCCAGCCTGCAGCAACACTGTGAAGCCTGGGGGGCCAGCCTGCCAGAGGCCCAGGTCT GGGGCTACTTGCGGGACATTCTTCTGGCTCTGGACCATCTACATAGTCAAGGCCTAGTTCACCTTGATGT CAAGCCTGCCAACATCTTCTTGGGTCCCCGGGGCCGCTGCAAGCTGGGCGACTTTGGACTACTGGTGGAG CTGGGTTCAACCGGTGCTGGCGAGGCCCAGGAGGGAGATCCTCGCTACATGGCCCCAGAACTGCTGCAGG GCTCTTATGGGACAGCAGCAGATGTGTTCAGTCTGGGTCTCACCATCTTGGAAGTGGCCTGTAACATGGA ACTGCCCCATGGTGGGGAGGGCTGGCAGCAGCTGCGCCAGGGATACTTGCCCCCTGAGTTCACTGCTGGT CTGTCTTCTGAGCTGCGTTCTGTCCTCGCCATGATGCTGGAGCCTGACCCCCAGCTTCGAGCCACAGCTG AGGCCCTGTTGGCCTTACCCATGCTGAGGCAGCCACGTCCCTGGAATGTTCTGTGGTATATGGCTGCAGA AGCCCTAAGTCGAGGCTGGGCCCTGTGGCAGGCCCTGGTCACTCTGCTCTGCTGGCTCTGGCACGGGCTG GTGCATCCTGCCAGTTGGCTGCAGCCTCCAGGCCCGCCGGCCACACCACCTGGCTCTCCACCTTGCAGCC CCCTCCTGGACAGCACCCTCTCCAGCAGCTGGGATAATGACAGCATAGGTCCCTCACTCTCCCCAGAGAC CGTCCTGTCCCGGATCACTAGAAGAACCTCTACCCCTCGGGGCAGGTACATACCTAGGGATGCCCTGGAC CTAACTGATGTGGACTCAGAGCCTCCAAGAGGTCCCTGCCCCACCTTTGAGCCAAGGAACCTCCTCAGCC TGTTTGAGGACTCCCTAGACCCAGCCTGAGCACAGGCCCAGCCAGCCTTGGTGCTTTTAAACCTCTGACC GTCTTGCCCACCTCTCCCCTGAAACTTCCGTGTCTAATAAAAAGTAATGCGTCTTGGGAACACCCAAGGT TGCTCATGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2609421 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2609421 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17262 same embryo
	EMAGE:17259 same embryo
	EMAGE:17264 same embryo
	EMAGE:17260 same embryo
	EMAGE:17261 same embryo
	EMAGE:17263 same embryo
	EurExpress:euxassay_001641 same experiment
	MGI:4827234 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source