EMAGE mouse gene expression spatial database

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EMAGE:17259

AI480653 expressed sequence AI480653 ( MGI:2146443)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001646. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001646_01	euxassay_001646_02	euxassay_001646_03	euxassay_001646_04

euxassay_001646_05	euxassay_001646_06	euxassay_001646_07	euxassay_001646_08	euxassay_001646_09

euxassay_001646_10	euxassay_001646_11	euxassay_001646_12	euxassay_001646_13	euxassay_001646_14

euxassay_001646_15	euxassay_001646_16	euxassay_001646_17	euxassay_001646_18	euxassay_001646_19

euxassay_001646_20	euxassay_001646_21	euxassay_001646_22	euxassay_001646_23	euxassay_001646_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17259_wholemount_strong.wlz

17259_wholemount_moderate.wlz

17259_wholemount_weak.wlz

17259_wholemount_possible.wlz

17259_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17259_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5678
Entity Detected:	AI480653, expressed sequence AI480653 ( MGI:2146443)
Sequence:	sense strand is shown >T5678 TGCTGCTCTGAGTGGGCCCGGCTGCTGGGCCGCCGGCTCCGGCGCATGGGCCTCCTCCGAGGCGGAGCTG CGTGCGCTCGGGCCATGGCGCGCCTGGGCGCGTTGCGCTCGCACTACTGCGCGCTGCTGCTGGCCGCGGC GCTGGCGGTGTGCGCCTTCTACTACCTGGGCTCAGGCCGCTTGCCCTTCTCCAGCGCCACCAAGAGGCTG AAGGAGGCCCGCGCCGGGGCCGCCGCGCCCACGCCGCCCGCACCCGAGCTGGCCCGGGGCTCCGCGGCGC CGGGCTTCGGGGCCAAGGCCAAGAGCCTGGAGGGCGGCGTGGTGGTCCCGGTGGACTACCACCTGCTGAT GATGTTCACCAAGGCGGAGCACAACGCGCCGCTGCAGGCCAAGGCTCGGGTGGCGCTGAGCTCCCTGCTG CGCCTCGCCAAGTTCGAGGCGCACGAGGTGCTTAACCTCCACTTCGTGAGCGAAGAGGCCAGCCGCGAGG TGGCCAAGGCCCTGCTGCGGGAGCTCCTGCCGCCCGCCGCCGGCTTCAAGTGCAAGGTCATCTTCCATGA TGTTGCTGTGCTGACAGATAAGCTCTTCCCCGTCGTGGAGGCCATGCAGAAGTACTTCAGTGCTGGCTCG GGGACCTACTACAGCGACTCCATCTTCTTCCTCTCGGTTGCCATGCACCAGATCATGCCCAAAGAGATCC CGAGGATCATCCAGCTGGACCTTGACCTGAAGTATAAGACCAACATCCGAGAGCTGTTTGAGGAGTTCGA CAACTTCCTGCCAGGCGCTGTTATCGGCATAGCCAGAGAGATGCAGCCTGTGTACAGGCACACGTTCTGG CAGTTCCGCCATGAGAACCCCAAGACCAGAGTTGGGGACCCTCCGCCTGAAGGACTCCCTGGCTTCAACA GTGGAGTGATGTTGCTGAACCTGGAGGCCATGCGCCAGTCTCCGCTCTACAGCCATCTGCTGGAACCTTC GTGGGTACAGCAGCTTGCTGACAAGTACCACTTCCGGGGCCACCTGGGGGACCAGGACTTCTTCACCATG ATTGGCATGGAGCACCCTGAACTCTTCCATGTGCTGGACTGCACCTGGAACCGGCAGCTGTGCACCTGGT GGAGGGACCATGGCTACAGCGATGTCTTCCAGGCATACTTCCGCTGTGAGGGCCACGTCAAGATCTACCA TGGGAACTGCAACACACCCATCCCAGAGGACTAGGCACCAGCTCACCCGCGATGCTGGTGGGGCTCCCGG GCCTGGGGAAGAAAGAGAGACTCTGCTAGGACACTCTTTAGGGGTGAGGTGCTGCAGACCTCCCAGCTGG CTGGCTCCTATCCAGCCAATATCCAAAGAAGCTTAGCTGAGCCTTGCTGCTGCTCGAGCCGTTTTCCTCC AGGGCAGCCCGAAGGACTGACACGGGTGTCTTCCAGGAGGTGGTCGAAGATACGGAAGGAAGGAGAATTG CACCCTTGTCCACACGGAATCAAAACCCTTGTAAAGAACTGGCTTTGCTAGACCAACTAAGCATTTCAAG CGGCAGGCTCTAATGCTTGGTGAGAGAAGAGGTGGGTGCTACTGTGGCTCCCAGTGAGCACTGAATGCCT AGTCAGGGTTTTAACAAGCAGCCCAGGGCTTGAGCCTCCAAAGGAATGCCACAGTCCCACATCCTTTCCC AGGAGGCTTTGTACCTCCTCCAGATGTTCATGTTGCTTGGTGCTGGCTTCAAGAGCAGCACCTCATTAGA TAAAGACTGAGCACATAAAAAAAACCCTTACTATAGTGTCCAGAGCTCTTATCCCTCCTCTATCCCAGAA CCCCACCTTCTCTTGTCTGTCTACCTGCTCCAGGTGACCTGACTTCTACTAAAGAATCAAACCCGTCATC CAAGGACAGAGGTTGGCCATGCCGGGGCATGCACTAGATTCACTCCAAGGAATTCACTCCAAGTCTAAAG TACTGGGTCACAAGTACAATTGGGGGGGCTTGGGAAAGGGTGGCCTCCTGCTCTCATTGTAGTCTCTGGA GAAGAAAGAAAACCAAACTCGTAATTGTCCACTGTGTCATGCCAGCCCCTACCCCTTCCCAGAAGTGAGT ACCATTTCTCTGTGTGCTTGAGACGGGCTTTGTGAGTCAGCTGCCCACATGAAGCCCCACAGGCCGTGTC TGCACAGTCCAGCTCATGTCCTCAGGCAAGAAATGGGAGATTCTTATTGTATATAGTCAGTACCATGAGC GACAGACACTAGCTGATGAATAGACTTTGAGAACTCTTGAGTCACGGTGTTTGTTCTCTGGCCAGTGTTC AGGGCTCTGGATGGGCAGTAGGATCAGCATCAAGCAGAAGGCCTAAGAGGCCCAACCGGCAGGCCTGCAC GTGGGCGCAGTGGGTGGTAGGTACTGCCCTGTTTGCTTCCAGGTGCAGAACTATCTCGTTCTAGCTCTTA GCAGGCTCATCCCCTGCCGGGAACAAGCTCAGAAGTCTCTGGCTCCTTAGCCCAGACCTCCCAGTTCCAT TCATGCTGGTGAGTGGCACACTCCTTCACTGGCCCTCTGCCGGGCCCAGTTCCTCTGTTCTCCTTGCTAC TTGATTTCCTATTCATAGTGTCTGGTTATTAGATGCCTGCGTGCGCCAGGCATCGTCTTCAGGATTTATA TGTGCCCTAGATAATATTCATGAAAACCTCATCCAGTTCACTCCAGCTCTAAAACTGATGGATGAAAACA GAGACACGGACAGGTTCTCTGCTGTGCTGTTTACTGAAGCTTTTTACTGTGCAAAAATGTTGGAAACAGC TTAAAATGTTGGCTAGAGAAGCAGATAAATAATGCATACTGCAACCGTATGGTGGAATATATCGCAAATC ATTTGAAGGAATATTTAATGTACCAGGAAATGCTTATGCTAGTGAGTAAAGAATACACAATAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2615706 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2615706 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17258 same embryo
	EMAGE:17262 same embryo
	EMAGE:17264 same embryo
	EMAGE:17260 same embryo
	EMAGE:17261 same embryo
	EMAGE:17263 same embryo
	EurExpress:euxassay_001646 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source