EMAGE mouse gene expression spatial database

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EMAGE:17308

Sardh sarcosine dehydrogenase ( MGI:2183102)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003731. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003731_01	euxassay_003731_02	euxassay_003731_03	euxassay_003731_04

euxassay_003731_05	euxassay_003731_06	euxassay_003731_07	euxassay_003731_08	euxassay_003731_09

euxassay_003731_10	euxassay_003731_11	euxassay_003731_12	euxassay_003731_13	euxassay_003731_14

euxassay_003731_15	euxassay_003731_16	euxassay_003731_17	euxassay_003731_18	euxassay_003731_19

euxassay_003731_20	euxassay_003731_21	euxassay_003731_22	euxassay_003731_23	euxassay_003731_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17308_wholemount_strong.wlz

17308_wholemount_moderate.wlz

17308_wholemount_weak.wlz

17308_wholemount_possible.wlz

17308_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17308_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5730
Entity Detected:	Sardh, sarcosine dehydrogenase ( MGI:2183102)
Sequence:	sense strand is shown >T5730 GGCTCCTCTGGCATGTATGCAGTGCCTGGCATGGTGTCCTAAGGTAGAGGCACTGTTTGGACTTGCAGAG CCCCGATTGGATCCATATCATGCTGACTTTTCCTTGCTAGGAACCTCTCAGGAGCTTCTGGAAGACCAAG GAACCCATTGAAAGACTAAGGTACAGGAGCCCTAAGTCCCCATGGCTTCACTGAGCCGGGTCCTACGTGT AGCTGCCACCTGTCCTCGAGGGAGAGCTGCTTGGAATCTGGGGCTACAACCCCTAGCCACAGAGGCCAGG CCCACCACCGAGAAGAGTGTTCCTTATCAGCGGACCCTGAAGGAGGAGGCCCAAGGTGCCTCCGTGGTGC CCCAAGGACCAAGCCAGCCCCTGCCTAGCACAGCCAATGTTGTGGTCATCGGTGGGGGCAGTTTGGGCTG CCAGACTCTGTATCACTTGGCTAAGCTGGGTGTAGGTGGAGCCGTGCTGCTGGAGAGAGAGCGACTGACC TCTGGGACCACCTGGCACACAGCAGGCTTACTGTGGCAGCTGCGGCCCAGCGATGTGGAGGTAGAGCTTT TGGCTCACACACGGCAAGTGGTGAGCCGTGATCTGGAAGAGGAGACTGGGCTGCATACAGGCTGGATCCA GAATGGTGGCCTCTTCATCGCCTCTAACCAGCAACGCCTGAATGAATACAAGAGGCTCATGTCGTTGGGC AAGGCCTATGGTATAGAATCCCACGTGCTGAGCCCAGCAGAGACCAAGAGTCTATATCCGCTGATGAACG TGGACGACCTCTATGGGACCCTGTATGTGCCACAGGATGGTACCATGGACCCCGCTGGCACCTGTACTAC CCTCACCAGAGCAGCAGTGGCTCGAGGAGCTCAGGTCATTGAAAACTGTGCGGTGACTGGCATCCGTGTG CGGACGGACGACTTTGGAGTGCGGCGGGTGGCAGCTGTGGAGACAGAGCATGGCTCCATCCAGACACCCT GTGTGGTCAACTGTGCAGGAGTGTGGGCGAGCAAGGTGGGCCGCATGGCTGGAGTGAAGGTCCCGCTGGT GGCCATGCACCATGCTTACGTCGTCACTGAGCGCATCGAGGGGATCCAGAACATGCCCAATGTCCGGGAT CACGATGCTTCTGTCTACCTCCGACTGCAGGGGGATGCCTTGTCTGTGGGTGGCTATGAGGCCAACCCTA TCTTTTGGGAGGAGGTGTCAGACAAATTTGCCTTTGGCCTCTTTGACCTGGACTGGGATGTGTTCACCCA GCACATTGAAGGCGCCATTAACCGTGTCCCTGTGTTGGAGAAGACGGGGATCAAGTCCACTGTCTGTGGT CCTGAATCCTTCACACCTGACCATAAGCCCCTGATGGGGGAGGCTCCTGAACTCCGTGGCTTCTTCCTTG GCTGTGGCTTCAACAGTGCAGGCATGATGTTGGGTGGTGGCTGTGGACAAGAGCTGGCCCATTGGATTGT GCACGGGCGCCCAGAGAAGGACATGTACAGCTATGACATCAGGCGTTTCCATCACTCGCTCACGGACCAC ACCCGCTGGATCCGAGAGCGCAGCCATGAGTCCTATGCCAAGAACTACTCGGTTGTCTTCCCACACGATG AGCCACTGGCAGGACGCAACATGAGGAGAGACCCCCTGCATGAGGAGCTGCTTGGACAAGGCTGTGTGTT TCAAGAGCGACAGGGATGGGAACGGCCGGGATGGTTCAACCCCCAGGAAACAGCTCAGGTCCTTGACTAC GACTACTACGGGGCCTACGGAAACCAGGCACACAAGGACTACACCTACAGCAGGTTGCTAGGCGATGAGT ACACCTTCGACTTCCCACCCCACCACCACATGATCCAGAAGGAATGTCTGGCCTGCAGAGGGGCTGCTGC TGTGTTCAACATGTCTTACTTTGGGAAGTTCTACCTGCTCGGGGTGGACGCCAGGAAGGCTGCAGACTGG CTCTTCTCAGCAGATGTCAACCGACCCCCAGGCTCAACAGTGTACACATGCATGCTGAACCAACGGGGAG GCACTGAGAGCGACCTGACTGTTAGCCGATTGGCTCCTGGCACCCAGGCCAGTCCCCTGGTCCCTGCCTT TGAAGGGGATTGCTACTACCTGGCCGTGGGTGGGGCTGTGGCCCAACACAACTGGTCCCACATAAACACG GTGCTACAGGACCAGGAGTTCCGGTGCCAGCTTATGGATAGTTCTGAAGATCTGGGCATGCTCAGTATCC AGGGCCCAGCCAGCCGTGACATCCTGCAGGATGTGCTTGATGCTGACCTGAGCAACGAGGCCTTCCCCTT CTCCACCCACCAGCTGGTGAGAGCCGCCGGGCACCTGGTCCGGGCCATCAGGCTGTCCTTTGTCGGGGAG CTGGGATGGGAGCTGCATGTACCCCGGGCATCCTGCTTGCCAGTGTACCGGGCTGTGATGGCTGCAGGTG CGAGGCATGGCCTAGTCAACGCAGGCTACCGTGCCATCGACTCCCTGAGCATCGAGAAAGGCTATAGACA CTGGCATGCAGACCTGAGGCCCGATGACAGCCCCCTGGAGGCAGGATTGGCCTTCACCTGCAAACTCAAG ACCTCGGTGCCCTTCCTAGGCCGAGAGGCCCTGGAGAAGCAGCGTGCCACAGGTCTCCGCCGACGCCTGA TATGCCTCACTGTGGAAGAGGAGGTGCCTATGTTTGGCCTGGAGGCCATCTGGAGAAACGGCCAAGTGGT GGGCCATGTCCGGAGAGCTGACTTTGGATTTACTGTGAACAAGACCATCGCCTATGGCTACATCCGAGAC CCCAGTGGTGGCCCTGTCTCTCTGGACTTTGTGAAGAATGGAGAGTATGCCTTGGAGAGAATGGGGGTCA CCTATGCTGCCCAGGTTCACCTGAAGTCTCCCTTTGACCCTGACAACAAGCGGGTGAAGGGAATCTATTG ATGGGATGCAGGTGGAGGCTACAGCTGCTCAGGCCACTGGCAACAGCCATGCTCCCCACACTGCCTGCGG ACCCCTGCACTGCACACACAGCCTTGTGTCCTGAGACCCAGAATAAAGGCAAAGCACAGACTTCCCCAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2648344 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2648344 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17311 same embryo
	EMAGE:17310 same embryo
	EMAGE:17307 same embryo
	EMAGE:17309 same embryo
	EMAGE:17312 same embryo
	EurExpress:euxassay_003731 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source