EMAGE mouse gene expression spatial database

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EMAGE:17310

Dhx16 DEAH (Asp-Glu-Ala-His) box polypeptide 16 ( MGI:1916442)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003719. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003719_01	euxassay_003719_02	euxassay_003719_03	euxassay_003719_04

euxassay_003719_05	euxassay_003719_06	euxassay_003719_07	euxassay_003719_08	euxassay_003719_09

euxassay_003719_10	euxassay_003719_11	euxassay_003719_12	euxassay_003719_13	euxassay_003719_14

euxassay_003719_15	euxassay_003719_16	euxassay_003719_17	euxassay_003719_18	euxassay_003719_19

euxassay_003719_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17310_wholemount_strong.wlz

17310_wholemount_moderate.wlz

17310_wholemount_weak.wlz

17310_wholemount_possible.wlz

17310_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17310_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5707
Entity Detected:	Dhx16, DEAH (Asp-Glu-Ala-His) box polypeptide 16 ( MGI:1916442)
Sequence:	sense strand is shown >T5707 GGCAGCCGGAGCGAGGCGGTAGTACCGCAGGCTCTGTCCCCGCCGCAGTCCTGGCCGTTCCGCGGTTGCG CGCCCGGAGCAGGACGCGTCGCCATGGCGACGCCGGCGGGGCTGGAGCGGTGGGTGCAGGAGGAGCTGCA CTCGGTGCTGGGGCTGAGCGAGCGGCACGTCGCGCAGTTCCTGATCGGCACCGCACAGCGCTGCGCCTCC GCCGAGGAGTTCGTGCAGCGCCTGCGGGACACCGACACCCTGGACCTCGGGGGGCCGGCGCGGGACTTCG CCCTGAAGCTCTGGAGCAAGGTGCCACGGAAGGCTGTGGTGGAAAAGCCAGCTCGGGCAGCTGAGCGAGA GGCCCGAGCCCTGCTAGAGAAAAACCGTTCTTATAAGTTGTTGGAAGACAGCGAGAGCGGCGAGGAGGCA GTGGGTAGTAACGGGAGCAGCCTCCAGAAGAAGCGGAAGAGACGGAAACACCTCAGGAAGAAACACCAGG AGGACGAGGAGGACGAGGAGGAAGAGGTTTCCGAGTCAGGGAAGAGGAAGGCAGGGGGGAGTAAATCACC CACGGAGGAGAAGCCAGCCTCAGAGGATGAATGGGAAAGGACGGAGCGTGAGCGTCTCCAGGACTTGGAA GAACGGGATGCCTTTGCAGAGCGGGTTCGGCAACGGGACAAGGACCGGACCCGGAATGTCCTGGAACGGT CAGATAAGAAGGCCTATGAAGAGGCTCAGAAGCGCCTCAAGATGGCTGAGGAAGATCGGAAAGCCATGGT CCCTGAGCTCCGTAAGAAGTCTCGTCGGGAATACCTGGCGAAGCGGGAGCGAGAAAAACTGGAGGACCTG GAGGCGGAACTGGCTGATGAGGAGGTCCTGTTTGGGGACGTGGAGCTGAGTCGCCATGAGCGCAGGGAGC TCAAGTACAAGCGACGTGTGCGGGACCTGGCTCGGGAGTACCGGGCAGCCGGAGAGCAGGAGAAGCTGGA GGCCACTAATCGCTACCACATGCCCAAGGAGACCCGAGGGCAGCCAGCTCGAACTGTGGACATCGTTGAG GAGGAATCGGGTGCGCCGGGAGAGGAGCAGCGGCGCTGGGAGGAGGCCCAGCTGGGTGCTGCATCCCTGA AGTTTGGAGCCCGAGATGCTGCTGCGCAGGAGGCCAAGTACCAGCTGGTGCTGGAGGAGGACGAGACCAT CGAGTTTGTCCGTGCTGCTCAACTCCAGGGTGACGAGGAGCTGTCAGGCCCGCCCCTGTCAGCCCAGGCC CAGCAGAAGGAATCTATCCAGGCTGTGCGCCGCAGCCTGCCCGTGTTCCCCTTCCGAGAGGAGCTTCTGG CCGCCATTGCCAACCATCAGGTCCTCATCATCGAAGGCGAGACTGGCTCTGGGAAGACCACACAGATCCC ACAGTACCTCTTTGAGGAGGGTTACACAAAGAAGGGCATGAAGATTGCTTGCACCCAGCCCCGGAGAGTG GCGGCTATGAGTGTGGCAGCTCGAGTGGCCCGGGAGATGGGTGTGAAGCTTGGGAACGAGGTGGGCTACA GCATCCGGTTTGAGGACTGCACCTCAGAGCGAACTGTTCTCCGCTACATGACAGATGGAATGCTACTCCG AGAGTTCCTCTCTGAGCCTGACCTTGCAAGTTACAGTGTGGTGATGGTGGATGAAGCTCACGAGCGGACC TTGCACACAGACATTCTCTTTGGATTGATCAAAGACGTCGCTAGATTCCGACCTGAGCTCAAGGTCCTGG TGGCTTCAGCCACACTGGATACTGCCCGGTTTTCTGCCTTCTTCGATGACGCCCCTGTCTTCAGAATCCC TGGACGCAGGTTTCCAGTTGACATCTTCTATACCAAGGCCCCAGAGGCTGACTACCTGGAAGCCTGCGTT GTGTCTGTGCTGCAGATCCACGTGACCCAGCCCCCTGGAGATATACTGGTGTTCCTGACGGGACAGGAGG AGATTGAGGCTGCCTGTGAGATGCTCCAGGACCGCTGCCGCAGGCTGGGCTCCAAGATCCGGGAGCTCCT GGTGCTGCCCATTTATGCCAACCTGCCCTCAGACATGCAGGCTCGCATCTTCCAGCCCACACCCCCCGGG GCCCGAAAGGTGGTTGTGGCAACAAACATTGCAGAAACTTCCCTCACCATCGAAGGCATCATCTATGTGC TGGACCCAGGGTTCTGCAAGCAGAAGAGCTACAACCCTCGTACGGGAATGGAGTCACTCACGGTCACCCC CTGCAGCAAGGCTTCAGCCAATCAGCGGGCTGGCCGTGCAGGTCGAGTGGCTGCCGGGAAGTGCTTCCGC CTGTATACGGCCTGGGCCTATCAGCATGAGCTAGAGGAGACCACAGTTCCTGAGATCCAGAGGACAAGCC TGGGCAACGTTGTGCTGCTGCTAAAGAGCTTAGGGATCCATGACCTGATGCACTTTGACTTCCTGGACCC TCCTCCATACGAGACCCTGCTGCTGGCTTTGGAGCAGCTTTATGCCCTTGGCGCCCTCAACCACCTTGGA GAACTCACCACGTCTGGTCGAAAGATGGCAGAGCTGCCTGTGGATCCCATGTTGTCTAAAATGATCTTGG CCTCCGAGAAGTATAGCTGTTCAGAAGAGATCCTGACCGTGGCTGCTATGCTGTCTGTCAACAATTCCAT CTTCTACCGGCCCAAGGATAAGGTTGTCCATGCCGACAATGCCCGTGTCAACTTCTTCCTCCCTGGTGGA GACCACCTGGTTCTGCTAAATGTGTACACACAGTGGGCTGAGAGTGGCTACTCTTCCCAATGGTGCTATG AAAACTTTGTACAGTTCAGATCCATGCGCCGAGCCCGGGATGTGCGGGAACAACTGGAGGGGCTCTTGGA GCGTGTGGAAGTTGGCTTGACTTCTTGCCAAGGTGACTACGTTCGTGTGCGAAAGGCCATCACTTCGGGT TATTTTTACCACACTGCGAGGCTGACTCGGAGCGGCTACCGCACAGTGAAGCAGCAGCAGACAGTGTTTA TTCATCCCAACTCCTCTCTCTTTGAACAACAGCCACGCTGGTTGCTCTACCATGAGCTTGTCTTGACCAC AAAGGAATTCATGAGACAAGTACTGGAGATTGAAAGCAGCTGGCTTTTGGAAGTGGCTCCCCACTACTAT AAGGCCAAGGAGCTAGAAGATCCCCATGCTAAGAAAATGCCCAAAAAAGTCGGCAAGACACGAGAAGAGC TGGGCTAGAGAGTGACATGGACCAAATCTTGCAGCTCTTTTTTCCTTCCATACTTTAATATCTAGTAAAT AAAATTATTTTTGGAATGAAGTGCATGGGAACTCTGGAACCCCAGAGAAAGTGACGTGAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2647184 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2647184 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17311 same embryo
	EMAGE:17307 same embryo
	EMAGE:17309 same embryo
	EMAGE:17308 same embryo
	EMAGE:17312 same embryo
	EurExpress:euxassay_003719 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source