EMAGE mouse gene expression spatial database

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EMAGE:17312

Zfp9 zinc finger protein 9 ( MGI:99210)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003736_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17312_wholemount_strong.wlz

17312_wholemount_moderate.wlz

17312_wholemount_weak.wlz

17312_wholemount_possible.wlz

17312_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17312_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5706
Entity Detected:	Zfp9, zinc finger protein 9 ( MGI:99210)
Sequence:	sense strand is shown >T5706 CCACTCCCCATCCCCGCTCTAAAACACTGCAGAAAATGACCAAGTACAAGGGACCCGTGACATTAAGGGA TGTTGCTGTGGAATTCTCCAAAGCAGAATGGAAGCTCCTGACCCCTGCTCAGAAGTCCCTTTACAAAAAC GTAATGCTGGAGACCTTCAGTAACCTGTTCTCTGTAGGTTATCGTGTGGTTAAGCCAAGTGTGATTGCAA AGTTGAAGAAAGGAAAAGAGCCATGGCCATTAGAAGAATTTTTCCGTAAGAGCCGTGGTGATAAGCGGCG GACCGCTCACGACCCCAGAGCCAAACATCAGGAGCAACAGGCTGGAAATGCAAGGAAAGGAGAACTTACA AAGCATCAGAAAACTCCCACCCGGCATGAAAGCTACGACTGTAGCGAATGTGGAAAATCTTTCTGCCAGA AGTCTTCCCTGCTGGCGCACCAGGAAACACACACCAAGAAGTCCTATAAATGTGAGAAATGTGGACAAGC TTTCTATAAGAACGAAGACCTCACAATCCATCAGAAAGTCCATACCAGAGATAAAACATACCCATGTAAA GAATGTAACAAAATTTTCTATCACCTGTCGTCTCTCACTAGACATCTGAGGATCCACGCTGGGGAGAAAC CCTACGAATGTAGCCAGTGTGATAAATCCTTCTATCAGAAGCCACACCTCACAGAGCACCAGAAAACACA CACAGGGGAGAAGCCATTTGAGTGTAAGGAGTGTGGGAAGTTCTTCTATGTGAAGGCATACCTCCTGGTA CACCAGAAAACACACACAGGCGAGAAGCCTTTTCAGTGTAAGGAATGTGGGAAGTTCTTTTCACAAAAAT CACACCTCACAGTACATCAGAGAACACACACAGGGGAGAAACCCTACAAATGTAAGGAGTGTGGGAAACT CTTCTCTAGGAATTCACACCTCAAAACCCATCAGAGAACCCACACAGGGGAGAAACCCTACAAATGTAAG GAGTGCGGAAACTGCTTCTACCAGAAGTCAGCCCTCACCGTGCACCAGCGAACTCACACCGGGGAGAAAC CTTTCGAATGCAGTAAGTGTGGGAAACACTTTTACTATAAGTCAGATCTCACCAAACACGAGAGAAAGCA TACAGGGGAGAAGCCGTACGAATGTGCAGAGTGTGGCAAATCTTTCTCTGTGAACTCAGTCCTTAGATTA CACGAAAGGACTCACACGGGAGAGAAGCCGTACGAGTGTGAGATATGTGGAAAGTCCTTCTCTCAGAAGT CCCATTTTGTCATCCATCAGAGAAAACACACAGGGGAGAAGCCCTATGAGTGCCAGGAGTGTGGGGAAGG CTTTATCCAGAAGTCACAACTCACGTCACATCAGAAGACACACTCCAAGAAGGGAAAGGCTAACAAGTAG CGCAAGTTCAGAACCCCTGCAGGAATTCACCCTTTGGCTGTAATACACACAGAGGGAGAACCTTCTAAAT ATGGAGGAAAAATGTCAACCATGTTCCCCCCGCCCCCTGAGAATTATCAGCAAATGTGCAGAAAGAAATT CTTCGATGTTATTAGGTAGACCTTGACAATAGGGTCAACTTTCATAAATATCAGGTGGCAGAAGACATTT TGCAAGCTACAAAATGTGAAAGAAACTTTTCTTTGAAAGAAACTTTCCTTTCAGAAGTCACACTGGTGCC ATATAGAAATAACACAGGGCAGAAATCCAGTAAGTAAGTGGATTTCCAGAAGTTTCTCTCATAGCATCTA GCATTACACATGCAGAGTTCACAGTGAGGAACTCTGGTGGGTATCAGGCACTCAGAAGTCTTCTTACAAG GTTGCACTCATCTTAAAAAGATGGAAGGGCATTCATGCATAGATTGCAAACAGATAGGAGAGCCTTATCA GGAACACGCCCATCGTGAAATGTTAATTGCATGAGTACTTTAATCATCCCTATGTACGTATTTTAAATAT TTGAACTGCCTTCAACAAAAATGCCCTGAAGGCATCGGTCACACTCTTAGTCCCAATGCCAGTTCCAAGG TTGTTTTTACTTCCTAAAGAAACATACAATATAAACAGAAACCAACTAATATCACATATATCAAAGGTAG TAAAAGAGCAAAACACAGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGT CCTGAGTTCAATTCCCAGTAACCACATGGTGGCTCACAACCATCTGTAATGGGATCTGATGCTCTTTTCT GATGTGTCTGAAGACAGCTACAGTGTACTCATATACATGAAATAAATACATAAATCTTTTAAAAAAAAAG AGCAAAAAACAATCAGAAAACATCATTAAACAAATAAGTCTTTTAATAAGTAGGATGTAGGGGTGTGTGT GTGTGTGTGTGTTTACCTATAAAAAAAATCTATCTAAAAAACTGTGTGTCTTGCTCTTGGAGCCCATTGT GGTTGTTCCTGAGCTCAGTAGCTGTGTAAGAACACATGTCACATTGTATCAACAGTGACTCCCACTGGTG GCATTGTTCCTGTAACATATATTGGTGTATACTTTAAATGCACAGTGTGTCTTTTTGTCCATCTCTGGGC ACTTACAAATGGGTTCAGACATTGTTACTAAATGGACCCTTCAAAAGAAACACTGTTTTTGTAACGTTTT CAGCTAAACTGTGTTGGGCTGAACTTCAGCTTGGACATGCATTTGAGCGGTCAGAGCTGTTTCTAAAGCA GCAGTATTTCCTTATTTTATTGTAGATGTAGCAGTGGCATCTGTGTTTGTATTGTGTATGAAAAATACCT CATGACTTTCCCTTCCTATTCTGGGTAGCAGAGGCAACATGACCAGTGGCTCAAAGTGAGATTCTAGGCT CTTACTACCTTCGTTGACCCAGATCCCAGCATGGACATACCATGTAACTTCACTGTGCCTCACCTTCTTC CTCTGTAAGAGAAATGACAGTAACATCCTAGAATTGCCCTGAGGTTGAAATACGTTGCCATTTTGAAGCA CTCAGAACAGTGTCTGTAAAACATAAGAACTCGACATTAGATAGTATTCCCATTAATTTGTATTTCCCGA ATCACATGGTGAAAAGGTTGGCAACCCAGAATTTATCTCCCATCTTATTCCTGTTAGTGCCTTGATTTGA TTTGGCTGTGTATCTCCCCACCGTGGCTCAGGAGCAGACCCTAAGTAGGCAAGGCCAGGGGTTGGTGAAC CGCAGCCCATCAGCCAGATCCACAGCCCGTTTTGTAGCCTGTAAGCTGAAATTAGTTTTATATTACTCAA TGATTGCAAAAATTAAGTATTTTGTGACACATGAAGGTTGTACAAAACAAAAGTTCCATATATCTAACTG AAGCACTTTGAGAACACTAATACAAACCCGTTCGCTACATTCTGCCTTTGCTGCCTTCGAACTGTGCTAG CGAGGCAGACCTAAGATGTTGCTGACTCAGCACTTTACCGCATCGGTTTCTGTTACTCAGGGCTGCACAC GCTGCAGCCGGTCCTGAGCCCTTCTTCTAAACGTTCTGTTTACCAAATGTGATGCCTCAGTGTATGGGTG GGTAATTTTTGTGCAGTATATTTATTTTGTGCATTGTATTTATATGTGGGAAAAGCCTAGGCTTTGAAGT ATTTTTTCCCAAAGAAATGTGGGTTTTTTTTTTTTTTTACACTTACTGAATTGTTGCGTTAAAGCATATC TCCTGATGGAATGTAATAAATATATGAAATAAACATTGTCTCAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2647280 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2647280 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17311 same embryo
	EMAGE:17310 same embryo
	EMAGE:17307 same embryo
	EMAGE:17309 same embryo
	EMAGE:17308 same embryo
	EurExpress:euxassay_003736 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source