EMAGE mouse gene expression spatial database

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EMAGE:17311

Igfbp5 insulin-like growth factor binding protein 5 ( MGI:96440)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001623. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001623_01	euxassay_001623_02	euxassay_001623_03	euxassay_001623_04

euxassay_001623_05	euxassay_001623_06	euxassay_001623_07	euxassay_001623_08	euxassay_001623_09

euxassay_001623_10	euxassay_001623_11	euxassay_001623_12	euxassay_001623_13	euxassay_001623_14

euxassay_001623_15	euxassay_001623_16	euxassay_001623_17	euxassay_001623_18	euxassay_001623_19

euxassay_001623_20	euxassay_001623_21	euxassay_001623_22	euxassay_001623_23	euxassay_001623_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17311_wholemount_strong.wlz

17311_wholemount_moderate.wlz

17311_wholemount_weak.wlz

17311_wholemount_possible.wlz

17311_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17311_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5732
Entity Detected:	Igfbp5, insulin-like growth factor binding protein 5 ( MGI:96440)
Sequence:	sense strand is shown >T5732 AAAAGAGCTAGGAAAGAGCTGCAAAGCTGTTTGGGCTTTTTTCCCCCTTTTTGTTCCTTTTTGTTACCCC CCCCCTCGGTCTGCACCCTTCTCCGGACTTCACGCAGAACCTGCGGGTTTCGAAGAGGTGGTGACAGAGC AGGTGTTGGGGTCCAGGTTTGGTGAGGTCTGGGTTTTTGCCCTTTTTCCCCCCTCGATTTCAACATTTTC CCGATCTTGTTGTCAGCCGCCGACGCCTCTTACCTGTTCTGCGGCAGCAGCGCAGCTGGCCGCTGAGACC GAGCGGAGTGGGGTTGCGTTTTAGATTTTAAGCAAAGGGGGGAAAATTAAGCCCAATCCATTTTTTTCTT CACCTCCTCCCTTTTCAAGGCCTCCAAGCTAATTATTTCTGTTGCTTTGGAGTGAGCAATTCTGTGGTTC TCTCCACCACCACCCCCAATTCTGACCCGATCCCGCCTGGGGGTTTCTACGGTCTCCGCTCGCTCTGCGT GCACCTGGCGCGCCTCTTTTTTTCACCCCCAACCTGTTGCAAGTCTTTAATCCTCGCAATTGGGACTTGC GTGCAGGCATCTGAATCCTCCTTGCCTCATATTTTGCAAGTGTTTGGGGGAGAGCACCTGCTCTACCTGC AAGAGATTTAAAAGGAAAAAAATCTCCAGGCTCCCTCTTTCTCCACACACTCTCGCTCTCCTGCCCCGCC CCGAGGTAAAGCCAGACTCCGAGAAAATGGTGATCAGCGTGGTCCTCCTGCTGCTGGCCGCCTATGCCGT ACCGGCTCAAGGCCTGGGTTCTTTCGTGCACTGTGAACCCTGCGACGAGAAAGCTCTGTCCATGTGTCCC CCCAGCCCTCTGGGCTGTGAGCTGGTCAAAGAGCCCGGCTGTGGCTGCTGCATGACTTGCGCCCTGGCGG AGGGACAGTCGTGTGGCGTCTACACGGAGCGCTGCGCCCAGGGTTTGCGCTGCCTCCCCCGGCAGGATGA GGAGAAGCCGCTGCACGCCCTGCTGCACGGCCGCGGGGTTTGCCTCAACGAAAAGAGCTACGGCGAGCAA ACCAAGATAGAGAGAGACTCTCGGGAACACGAGGAACCCACCACCTCCGAGATGGCTGAAGAGACCTACT CCCCCAAGGTCTTCCGGCCCAAGCACACTCGCATTTCCGAGCTGAAGGCTGAGGCTGTGAAGAAGGACCG CAGAAAGAAGCTGACCCAGTCCAAGTTTGTGGGGGGTGCAGAGAACACTGCCCACCCCAGAGTCATCCCT GCACCTGAGATGAGACAGGAATCCGAACAAGGCCCCTGCCGCAGACACATGGAAGCTTCCCTCCAGGAGT TCAAAGCCAGCCCACGCATGGTGCCCCGTGCTGTGTACCTGCCCAACTGTGACCGCAAAGGATTCTACAA GAGAAAGCAGTGTAAGCCCTCCCGTGGCCGCAAACGTGGCATCTGCTGGTGTGTGGACAAGTACGGAATG AAGCTGCCGGGCATGGAGTACGTGGATGGGGACTTTCAGTGCCACGCCTTCGACAGCAGTAACGTTGAGT GACGCGTCCCCTCCCTTCCTCCCCCTATCCTACCCCCCCAGCCCCAACTCCAGCCAGCGCCTCCCTCCAC CCCAGGACGTCACTCATTTCATCTCATTTAGGGGAAATATATATACATATATATTTGAGGAAACTGAGGA CCTCGGAATCTCTAGCAAGGGCTAAGGAGACACTCCCCACCATGACCCCGGAAATGTATTCCTTTTTGAA GCAAGTTGAACGGACAGAGAAGGGAAGGAGAGAAGAAGCAAGAGGGAGCGAGAGATGGAAAGAAAGCAAA GCGTTGGAATAGAGGAAAAGAGGGAAGGACAGATAGGATTAGAGAGAGAAGAGAGAAACAGCAAGGCAGA AAGGACTCCACAACCAAGGCTGAATCTGCCCTTTTGCTTTCAGCTCTAGCCTGGGGTCAGAAAAAGTGTG GCATTCAGTGACACCCAGTTTAGATTGGTCAAGGGGAGAAAAGAAACAAGGTGTGTCAGTGCCTCTCGGG TCTGTCCCCTCCTGCAGCCAGCAGTGTGGATGGCTAGACCCCTCACCCTCCTCTCCTCTTACCCAAGTGC AGGGTGATTTCATCCCCAAATTTACAAAGACTAAAATGCATTCCATCCCTCTGAAAATAAACAAAAGTGA GTGATTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2648602 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2648602 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17310 same embryo
	EMAGE:17307 same embryo
	EMAGE:17309 same embryo
	EMAGE:17308 same embryo
	EMAGE:17312 same embryo
	EurExpress:euxassay_001623 same experiment
	MGI:4825540 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source