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EMAGE:17622

Ndufs6 NADH dehydrogenase (ubiquinone) Fe-S protein 6 ( MGI:107932)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622
"Pseudo-wholemount" of euxassay_005964. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005964_01 euxassay_005964_02 euxassay_005964_03 euxassay_005964_04
EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622
euxassay_005964_05 euxassay_005964_06 euxassay_005964_07 euxassay_005964_08 euxassay_005964_09
EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622
euxassay_005964_10 euxassay_005964_11 euxassay_005964_12 euxassay_005964_13 euxassay_005964_14
EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622
euxassay_005964_15 euxassay_005964_16 euxassay_005964_17 euxassay_005964_18 euxassay_005964_19
EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622 EMAGE:17622
euxassay_005964_20 euxassay_005964_21 euxassay_005964_22 euxassay_005964_23 euxassay_005964_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17622Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17622_wholemount_strong.wlz
17622_wholemount_moderate.wlz
17622_wholemount_weak.wlz
17622_wholemount_possible.wlz
17622_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17622_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T10028
Entity Detected:Ndufs6, NADH dehydrogenase (ubiquinone) Fe-S protein 6 ( MGI:107932)
Sequence:sense strand is shown

>T10028
ACCGGCAAGATGGCGGCGGTGCTGACCTTCCGCCGGCTGTTGACTCTCCCCAGGGCTGCACGGGGTTTCG
GGGTTCAAGTGTCGCCGAGCGGGGAAAAGATCACGCATACCGGCCAGGTGTATGATGAAAAAGACTACAG
GAGGGTTCGTTTTGTAGATCGTCAGAAAGAGGTGAATGAGAACTTTGCCATTGATTTGATAGCACAACAG
CCTGTGAACGAGGTGGAGCACCGCATCATAGCCTGCGATGGAGGCGGTGGTGCCCTGGGCCACCCCAAGG
TGTACATAAACTTGGACAAAGAAACAAAAACGGGGACATGTGGCTACTGCGGCCTGCAGTTCAAGCAGCA
CCATCACTAGTGTGGGCTGTGTCCTGGTCCTCTGACTCCTATGGAACATCTCCACGCTGGGTGTTCTGTG
TGAGGCCACTGCTCTGTGAATGGTGTCCCTTGTTTTGAATAAAGGATGCTCCCACCATGAAAAAAAAAAA
AAAAAAAAAAAAAGAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6760537 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6760537 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17623 same embryo
 EMAGE:17624 same embryo
 EMAGE:17625 same embryo
 EMAGE:17626 same embryo
 EurExpress:euxassay_005964 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS