EMAGE mouse gene expression spatial database

EMAGE:17626

Ndufa12 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 ( MGI:1913664)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005963. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005963_01	euxassay_005963_02	euxassay_005963_03	euxassay_005963_04

euxassay_005963_05	euxassay_005963_06	euxassay_005963_07	euxassay_005963_08	euxassay_005963_09

euxassay_005963_10	euxassay_005963_11	euxassay_005963_12	euxassay_005963_13	euxassay_005963_14

euxassay_005963_15	euxassay_005963_16	euxassay_005963_17	euxassay_005963_18	euxassay_005963_19

euxassay_005963_20	euxassay_005963_21	euxassay_005963_22	euxassay_005963_23	euxassay_005963_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10058
Entity Detected:	Ndufa12, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 ( MGI:1913664)
Sequence:	sense strand is shown >T10058 GTTTTACCCGAACACGGAAGGGGCGGACCATGCAGATGAGAATGGAGCTGGTGGAGGTCCTGAAGCGCGG CGTGCAGCAGGTCACCGGCCACGGCGGCCTGCGGGGCCTCCTACGGGTTTTCTTCAGGGCAAATGATATA AGGATTGGTACACTGGTGGGAGAAGACAAATATGGAAATAAATACTACGAAGACAACAAGCAATTTTTTG GCCGCCACCGATGGGTCATCTACACCACCGAGATGAACGGCAAAAACACATTCTGGGATGTGGATGGAAG CATGGTGCCTCCTGAGTGGCACCGCTGGCTTCACTGCATGACTGACGACCCTCCGACGACTAATCCACCG ACTGCTCGTAAATTCATCTGGACAAACCATAAATTCAATGTGTCTGCCACTCCAGAGCAATACGTTCCTT ATTCCACCACTAGAAAGGAGATTCATGAATGGGTTCCACCTTCGACACCTTACAAGTGACCGCAGTGAAG ACTGTTCCTGCACTTCCAGGCTAGTGCAGGACCATCCTAGCAGCTGACCATGAACTTGATTAAAATTGTT TGACCTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6751147 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6751147 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17623 same embryo
	EMAGE:17624 same embryo
	EMAGE:17625 same embryo
	EMAGE:17622 same embryo
	EurExpress:euxassay_005963 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source