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EMAGE:17625

Mrpl47 mitochondrial ribosomal protein L47 ( MGI:1921850)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625
"Pseudo-wholemount" of euxassay_005962. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005962_01 euxassay_005962_02 euxassay_005962_03 euxassay_005962_04
EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625
euxassay_005962_05 euxassay_005962_06 euxassay_005962_07 euxassay_005962_08 euxassay_005962_09
EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625
euxassay_005962_10 euxassay_005962_11 euxassay_005962_12 euxassay_005962_13 euxassay_005962_14
EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625
euxassay_005962_15 euxassay_005962_16 euxassay_005962_17 euxassay_005962_18 euxassay_005962_19
EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625 EMAGE:17625
euxassay_005962_20 euxassay_005962_21 euxassay_005962_22 euxassay_005962_23 euxassay_005962_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17625Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17625_wholemount_strong.wlz
17625_wholemount_moderate.wlz
17625_wholemount_weak.wlz
17625_wholemount_possible.wlz
17625_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17625_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T10065
Entity Detected:Mrpl47, mitochondrial ribosomal protein L47 ( MGI:1921850)
Sequence:sense strand is shown

>T10065
ATTTAGAAACATGGCTGCGACCAGTCTAGTGGGTATTTGTAGAAGAGCCTCAGCGTTCCTGAAGGCAGCT
TGTTCCCTAGTAAATCCCAAGGACGCTGCTCACTCGGGTTGCAGGTCTTCTCTTAGTTTGTTACATAAGA
ACACACCACATGTCACATCTTTTCTCCAGTGTAAATTACTTCATACCACGTTGTCAAGGAAAGGACTGGA
AGAATTTTTTGATGACCCAAAGAATTGGGGGGAAGAAAAAGTCAAATCTGGAGCTTCATGGACCTGCCAG
CAGCTGAGGAACAAAAGTAACGAAGACTTACATAAGCTTTGGTATGCCCTTCTGAAGGAAAGAAACATGC
TTCTAACTCTGGAGCAGGAGGCCAAGCGACAGAGGTTGCCAATGCCAAGTCCGGAGCGCTTAGAAAAGGT
CGTTGATTCCATGGATAACGTAGATAAAGTTGTCCAGGAGAGGGAAGATGCTCTAAGGCTTCTTCAGACC
GGTCAAGAAAAGCCCAGACCCGGTGCTTGGAGAAGGGACATCTTTGGACGAATTGTCTGGCACAAATTCA
AGCAGTGGCCTATACCTTGGTACCTAAATAAAAGATACAACAGGAGGCGGTTCTTCGCAATGCCTTATGT
GGATCGCTTTATCAGACTAAGAATTGAGAAACACGCCCGCATTGAAGCAAGAAAGAGAAGTTTACAGAAA
AAGAAAGAAAAAATTCTCCATGCAAAGTTCCCACATCTCTCTCAAGAACGGAAATCAAGTAGTGTCTAAG
ATGAGACCCAGAAGTCTGGTTCCATTGTTCTTAAAGCCTGTGTCCTGACGTGAATACCTGTTAAGCAGTT
TATAAAGAAATGTTCTTGTCAAGTGAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30119240 using vector (pDONR 201) specific primers. Forward Primer - name:T7-pDONR 201-fw, sequence:CGTAATACGACTCACTATAGGGCTCGCGTTAACGCTAGCATGGAT; Reverse Primer - name:T3-pDONR 201-rv, sequence:CAAATTAACCCTCACTAAAGGGGTGCAATGTAACATCAGAGA. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30119240 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17623 same embryo
 EMAGE:17624 same embryo
 EMAGE:17626 same embryo
 EMAGE:17622 same embryo
 EurExpress:euxassay_005962 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS