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EMAGE:17624

Ppp1r26 protein phosphatase 1, regulatory subunit 26 ( MGI:2685193)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624
"Pseudo-wholemount" of euxassay_005960. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005960_01 euxassay_005960_02 euxassay_005960_03 euxassay_005960_04
EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624
euxassay_005960_05 euxassay_005960_06 euxassay_005960_07 euxassay_005960_08 euxassay_005960_09
EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624
euxassay_005960_10 euxassay_005960_11 euxassay_005960_12 euxassay_005960_13 euxassay_005960_14
EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624
euxassay_005960_15 euxassay_005960_16 euxassay_005960_17 euxassay_005960_18 euxassay_005960_19
EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624 EMAGE:17624
euxassay_005960_20 euxassay_005960_21 euxassay_005960_22 euxassay_005960_23 euxassay_005960_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17624Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17624_wholemount_strong.wlz
17624_wholemount_moderate.wlz
17624_wholemount_weak.wlz
17624_wholemount_possible.wlz
17624_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17624_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9496
Entity Detected:Ppp1r26, protein phosphatase 1, regulatory subunit 26 ( MGI:2685193)
Sequence:sense strand is shown

>T9496
GAGGAGCTCAGTTCTGGGTCCATCGTTATAAGCTGTGAGCAGACAGTTTGGTCTCACTAGAGACCCCTGG
CTAAGCCAGGAACTCACTATGCATAGACCAGGCTATCCTGGTGCTTGGACTAAAAGTGTACACCACCATG
TCCAGCCCTCACACGGCCGACTTTAAAAGCTGGTAGAAGATGCTTTGAAGTGCAAGCCGAGCAGTGAAGA
CCTCACCTGGAAGCCTTGCAGAGCATCAGCCTACACCTGAGACTTACAAGATGTGAAACAGGCTGACCCA
TAAGCCCTGCCTCTCAGCACCCAGCCTGCCTGCTAGGATGTTCCTCATGAACGCCCCTCCAGTGGTGGCG
CTCCAGTCCAGATGGGAGGCCTTTGGGCAGCCCAGGAGCTTCTGCCTCCCAGACTGCTTCTCTGAGGCTA
AAGAGGACGGCTCCAGGGCTTCCGTGAGCGCCAGGGTCCAGATGCTCATCAGCACGCTTCAGAGGGACGA
GGCTGCCCTGGGCATGGGCCATGAGCGTCTCACCCAAAGAGGCCAACGTGCAGAGAGGTCCCGGGACACG
AGGCTGGCCCCCAAGCCTGCTGTATGCAAAGAGCAGCCAGAGTTCCCTGCCCGTGGTCTCGTGGCAAACT
GCAGCGCCCTGGAGAAAGATGAAGCTGGGAGACGCAGTCCCTTGGAGCTGGATTCTGACAGTGATGACTC
CGTGGACCGAGACATTGAGGAAGCTATCCAAGAGTACCTGAAGGCAAGGGGTGGGGCTTCTGAACCTATG
TCCCAAGGGGCCCCCTCTATCCCCGAGCCTGCCCACAGCAGCACCTTGCCCATCCCGTGTCCCTCACAGC
TCACTCCCGGCTCAGGTAGTGTTCCTGTGGGAGCCAGTGAAGATCAGGGCTCCACCTCCCCAGCGAGCAT
GAGCAGCGAGGACTCCTTTGAACAGAGCATCCGAGCTGAAATAGAACAGTTTCTTAATGAGAAAAGACAG
CATGAAAACCCGAAGTGTGATGGGTTTGTGGATAAAAAATCCGACCCAAACAACAGCCCTGCCAGACTTA
GAGGGAACCGAGAAACCTCAGCCAGGGCAGCTCTGATGGGAACGTGTAAGGAGTTCATCTTCCGAAAACC
TCCCAGGTTAACAAAGATGAGCACACAGCAGAGAAACTTCCAGCCTAAGCCCACCACAGAGCCTGAGACC
CCAGTGAGCACGAAGCTGACCGCCCACAGACCGGAGGCTGCACAGAGCAGGGGTGGGGTGAGGAGGAGCA
TGCCTGCCAGGCGCAGCAAGCGCATCAGGAGCTCGGCCCCAGTGCACCAGGCATCAGACTCCAGCAGTGA
CGACGGCATTGAGGAGGCCATCCAGCTATACCAGCTAGAGAAAACCAGGAAGGAGGCCAGTGGGGACCCA
CCTCTGAGAGGCCAGCTGAAAGAGGAAAGCCCTGGTAGTGCCCAGCCAAACGCCTTGCCTGAAGCCCACA
GGAGACCCCCTAGCAAGAAAAAGCTGGCTGTTCCCAAGGTTATAGACACCACCCAGGGGGTCCTCCACCC
TGATCCCCTCTCCAGGCTCCTGACGGATTCGAGAGCCTCTTTACCTCCAGGACACGCAGCTGCCAAGAGT
GAAGCTGTGTGCCAGGCCTCACGCCTAGCAGACACATCTACCGAACTGATGTGTGCAGAAGCGATCCTGG
ACATCTCCAAGACCATTCTGCCAGCCCCCATGGAAGGCAGCGACAGGCCTCCATCCAGGAACCCACTCTT
CTGTCCCCAACCCATGCCTCCCCGTTCTGAAGGTGACAGCAGCAACATTGACAGTGATGATAGCATCGAG
CAAGAGATCCGAACGTTTTTGGCTCTCAAGGCACAAGTAGGGAGTCCTCAGCCTGCCCAGGGTCCACTGT
CTTCGCCTGGCCCCAGTGGCCAGCCTGGCATCCCCAAGGTCCCTTTTGCTAAAACACTAGATCTCCCTCT
GGTCTGCAAACGGAAACGGAGAGGTGGAGGAGGCAGCACTACAATGCCCAAGAAAATAAGGGAGGGTAGA
GAAAGTACCCAGGACGCTGACCACATCCAGGGGAAAGCTCAGCCTGGCCATGACGGGTGGGACCCTCTCG
GGCACAACAAAATCACAGAGACCCCAGGAGGAGAGGCTGAAGCCAAGGAACAGCCTGTCATTTCCAGGAC
AGTTGGGCTCAGTGATACACACCTACCACAAGGGGCCCTCGGGAAGGCCACTGAGAAGGAAAGCTCTGAG
GATAAGAGCAGTTCTCTGGACAGTGACGAGGACCTGGACATGGCCATCAAGGACTTGCTAAGATCCAAGC
GCAAGTTTAAGAAGAGGTGCCGAGACCCACGGGCTTCCTGTAAGAAGGTCAGATTTGGCAGCACAGAGAC
TCGGTGTGGGGAGAAGCCGAGCAACCTTCCAGGGGACTGGAAAGACCACAGACAGCAGGCACTACGGAGC
TGTCTGCCCAAGTGCAGAGGGGACAATAAAGATGGCCCAGGACGAAGCCCAGGAAGCAGTGTGGCAGAGA
AGGCAAAGATGGGTGGCACAGGGGGCGAGGACGCAACCCCTGCCTTTCTCCTCAGGAGGAAAAGTCCAGA
AGGGGCTCTTCCCTCTACAGACACAGGAGCCAGTGGCCACCCTCCTTCAGCCTCCAGCCCCACATCTGAG
GACAGTGCAGTGGACAGTGATGACAGCATCGAGCTGGAGATCAGGAGGTTTTTGGCAGAGAAGGCCAAGG
AGTCTATACGCAACACAGAACCTCAAGGGGGACCTGCCAAGCCAGAGATGCCCTGTAGGAAAGACCCAAC
CCTGGGGTTGCAGCCTGGAGTGTGCACACGGAGCCAAAAAGCCAGGGGAACCCCTCAGCTGGCCGAAGGA
AGGAGAGGCCCAGAGAGAGCTCGGACACAGGCAACCGGCCTCTTGAGCCAGTCTGGGAAGGGCACCCTCC
GTGCTGAGCAGACCGCCCGCCTCACCACTGCCCTGGGCCGAAGTGAGCCAGCACTACCCAAGAACACCTG
CAGGAACAGCTCTGCCAAAGCATCTCCGCCAAGCAGGAAAAGTGCTCACGTTCACAAAGACCATAGCCCA
CAGGGAAGCCAGACTGCAACGGCAGAAAGTGTGTTCGGTCAGCTGCCCAGTTGTGCCAAAGTGGGTGCCG
AGGCTGGAAGCGCCGGGGGTACCTTTCACCTGAACTATGGCAGTCAGAATTTGCTAACCCCCAACCCTGG
GTCCCAGGCTGACCTCGTCCTTCCTTGGAGTGACTTTGCACACCAGAGCAGGCTATCCAGCCCATGGGTG
CTGAACTCGGGTCAAGGCACAGTGTGGACAGGGGTCTTTAGGGGTGAGAAGGAAAAAGGGGCTACATCAC
AGGCTGGGGCCCCACCCAGCCTCTCCTCAGGCCCCAGGAAGGGCCTGCCTTTCCTCTCCACCCAGCTCTT
CCACTTTGGGAAGAATGTTTCTTGGGGGGGCAAGCAGACCAGCCTCTTCAGCCCCAATCTAGGCTTACCT
CTGCAGGCTCCCGCATTCTCAGCCTTCAGGGAGACCCAGCCTGGCCACAACCCTGTATTTGGAAGCCCAC
ACTTGCTAATGAAAGACAGTGGGAACTGGCCAAGCCGGAAGGCTCAGGGGACCCTGAGACAACAGGACAG
GAGGAATTCTGCCTCTGAGGACAAAGTTCTAGACCTGAGATATCGGCACAGGGTTGACAGGGAACCGCAG
GACCAGGAGACCTTGGGCAGTGATGCCAGCGAATTCAGTGACACTTCCATGGAGGATGGGGGCAGTGCCA
CAGTGAGCAGTAAAGGCCTCAAGTTGTGACAGGCATGCCATGCCATGGCTGTGGGAAGCAGTGTCCGTGT
CTTGCCTGATGTGTGTGCATGCATGCCTGTCTGTATGTGTGTGACTTTGTGTTTATGTGCGTGCGTGAGC
CTCTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGTGC
CTGTGTGTTAGGCAGCATAGGGTGGAAGTAGGGGATCATGACAGATAAAGAGCAAAAATTGGGTCCCTTC
CATGCAGCTCCCATCCACCCCTAATATAAATATAAATATGTACACTAACTTCAAATGACATTTTTATTTA
ATGAATGTGTCCTGGTACATAATGGGTTTTGTAGGTTTTTTGTAAATTATTTTAAAAGTGCTGTAAAAAA
TACTTTTTGAAAATGTTCAGTCTTGCAGGGCGTTCCTAGCTGCAGTTCTGGGTCTCAGTGCATGTGTGGT
AGGTGAGTAGACCCTTGGTTCTGCCTGTCCCTGTGAGGGTCAGAGACAGAGGCTGGAACCGCTTTGCTTG
TGAAGATATTCAGGCCTGGAGATCGCAGCAACCATTTACCTTGGAAGTGCTAATGTGGTTGAGGTATGTC
AGGATACCACTGGGGCAAGGGTGTCCCTGGATTCTGTAGTCATGACCTGTAATTAGAAAACATTTCAAAG
CACAACAGCTAGCAAATAGCTAACCATCCATCCCATCTAGTGTGGGAATCACTACTGGCTCTGTAGCTCA
GCTGACCCCAGATCATCCAGTATCCAGTAGCTGGGGGATTCTAACATCCTTATAAATCCCTTCATATAAG
GGCTACAGATTTGCTTTTAATCATGGCTCTAAAAGGTGATCCCACATATCCGTTTTGACTGTCCTGTTTG
TGATGTGTGTATGGGTGAGTAGTGAGGCCATGAGATGGCTTGGTCTCCAAACACCTTAGCCACCTGTGAT
GGTGAGCCCCAGCCCTGGAAGCAGTGAGGGCAAACCCTCCCACAGCTGCTGTGGGTGACCAGTGATGACA
CCAGCAGGCTGCATGGCTTTCTGCAATGCTGCCTCTACCCAGGAGAAAGAAGGCTCTGGCCAGTGCTGAG
GCCAGACCGCAGGCACTGGGGTCCTGGGAGTTTGGCAGTGATACTGCCTTGGTGCTGAGGTGCCTGTGGC
AACTGGGAACTGAAGATGTGCTGGAGCCCAGATGTGAAGATTACATCTGTGTTGTGATTCCACCTGTCTT
CCCTGAGTTCTGCAGCTCAGTATTCTGGATTGCTGTGTGGCTACTTTGTGGCTGAATTCTATTGGTATTC
TGACATGGGAGAGAGTTTTCTACTTTATGCACCTGGGTCTGAATTGTAAAAAGTGCCCAAATGCTGGCAC
ACACTCCAACCATCCTCCCCATCTGCCACGTTGGAACTTTGATGTGGTGGCAGGCTCTGTGGTCCCGTTG
TGGTCCTAAATGATCTGGTTCTATGTATAGTAAATTCATCGTGATATTTGTTCGTGTGTTTCTAGGAGAA
ACAGCTTTGTGTCGTCATCCATGTTCCCACTTTAAATTTTCTGAGTGCTTTTAGCCCCTGAAAACAAAGA
GCATCTTGTTTTGTATATAAGACCCATCCAGTTTGGGTTTTTGTTTTTCTAATGAATAAACCAAGTACTA
TCCACAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30362439 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30362439 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17623 same embryo
 EMAGE:17625 same embryo
 EMAGE:17626 same embryo
 EMAGE:17622 same embryo
 EurExpress:euxassay_005960 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS