EMAGE mouse gene expression spatial database

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EMAGE:19470

Serinc3 serine incorporator 3 ( MGI:1349457)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004869. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004869_01	euxassay_004869_02	euxassay_004869_03	euxassay_004869_04

euxassay_004869_05	euxassay_004869_06	euxassay_004869_07	euxassay_004869_08	euxassay_004869_09

euxassay_004869_10	euxassay_004869_11	euxassay_004869_12	euxassay_004869_13	euxassay_004869_14

euxassay_004869_15	euxassay_004869_16	euxassay_004869_17	euxassay_004869_18	euxassay_004869_19

euxassay_004869_20	euxassay_004869_21	euxassay_004869_22	euxassay_004869_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19470_wholemount_strong.wlz

19470_wholemount_moderate.wlz

19470_wholemount_weak.wlz

19470_wholemount_possible.wlz

19470_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19470_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

moderate

homogeneous

moderate expression: see section 09 10 12 20 21 weak expression: see section 11 18 19

thymus primordium

strong

homogeneous

strong expression: see section 13 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 05

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 09 10 moderate expression: see section 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 21 22 moderate expression: see section 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 16 17 18 19 20 21

mandible

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22

maxilla

strong

regional

strong expression: see section 04 05 06 07 08 09 10 16 17 18 19 20 21

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23

testis

strong

regional

strong expression: see section 07 08 09 20 21

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6019
Entity Detected:	Serinc3, serine incorporator 3 ( MGI:1349457)
Sequence:	sense strand is shown >T6019 GGCGACGCCTCAGTCGGCAGTTAGGATCGGCTCGGAGCGGTGGGTCTCGGCCCCGGCGTCACCATGGGGG CCGTCCTCGGCGTCTTCTCCCTCGCCAGCTGGGTCCCGTGCCTCTGTAGTGGTGCATCATGTCTGCTGTG CAGTTGCTGTCCCATCAGTAAGAATTCCACTGTAACTCGGCTCATCTACGCTTTTATCCTCTTCCTTGGC ACTATTGTGTCTTGCATCATGATGACAGAAGGCATACAAACTCAACTGAAGAAGATTCCTGGATTCTGTG AAGGAGGATTTCAAATCAAGATGGTTGATACAAAGGCAGAGAAAGATTGTGACGTGCTGGTCGGTTTTAA AGCTGTGTATCGGATCAACTTTGCTGTGGCCATCTTTTTCTTTGCCTTCTTTTTGCTCATGTTAAAAGTT AAAACAAGTAAAGATCCCAGAGCAGCAGTGCACAACGGGTTTTGGTTCTTCAAAATCGCTGCCATTATTG GTATCATGATTGGATCTTTCTACATCCCTGGGGGGAGTTTTACTGAAGTCTGGTTTTTTGCTGGAATGTT GGGGGCCTCTTTCTTCATTATCATCCAGCTGGTGCTCTTGGTAGACATGGCTCACTCTTGGAATGAATTA TGGGTAAATCGAATGGAGGAAGGAAACCCAAGGCTCTGGTATGCTGCCTTGCTGTCCTTTACAAGCCTCT TTTACATCCTCTCCATCGTCTTTGCCGCGCTGCTCTACGTCTTTTACACCAAGCCTGACGACTGCACAGA AAACAAGGTCTTCATCAGCCTCAACCTGATTTTTTGTGTTGCAGTTTCTATTGTGTCCATCCTCCCTAAA GTTCAGGAACATCAGCCTCGCTCTGGCCTCCTGCAGTCCTCCATCATCACTCTGTACACCCTTTACCTCA CGTGGTCAGCCATGACCAATGAACCTGAGCGGTCCTGCAATCCCTCCCTTATGAGCATCATCACACACTT AACTTCACCAACTGTGTCTCCTGCAAATTCAACTACTCTTGCTCCTGCCTATGCTCCGCCGTCACAGAGT GGACACTTTATGAATTTGGATGATATTTGGGGACTGATTATCTTTGTTTTCTGCCTTATATATTCTAGCT TCCGTACTTCGAGCAACAGCCAAGTTAACAAGCTGACCCTCTCTGGGAGTGACAGTGTTATCCTTGGTGA TACCACCAATGGAGCCAATGATGAAGAGGATGGACAGCCACGGAGGGCTGTAGACAATGAGAAGGAGGGG GTGCAGTATAACTACTCCTTTTTCCACTTGATGCTCTGCTGTGCCTCCTTGTACATCATGATGACCATAA CCAGCTGGTACAGCCCTGATGCCAAATTCCAGAAGGTATCCAGCAAGTGGCTAGCTGTGTGGTTCAAAAT GGGCTCCAGCTGGTTGTGCCTCCTCCTTTACCTCTGGACTCTTGTGGCTCCCCTGGTCCTCACAGGTCGG GACTTCAGCTGAGCTCAGTGTGTCAAGGACACTGATAAAGCTGACCAGAGTCTCCTTTTCTGAAAATGCA TATCCATTTTACGTTTCATCAACGAGACTATTAAGTGAACGCTTTGCAGATTTGGCTGTATTCAGGTTTA TATCAAAAGGCAAGATTGAGTAATGCTTGATGCAGAATCTGAGCTTTCATATATATATATATATATATAT ATATACACACACACACACACATATATGTTTATTTGTAAGGCTATAGCACAAAGGGAACATTTTTGTGTTT TAACATGAACTACAGCTGTGCTGTGAAGAGAATTCTTTATAAAGACCTGTAGATTCCTACAACTTTGGTT TAAGTTTTAAGTTAGAAGATTGTTGGATATTTAAGGCTGTCTTTAATTTCTATTACAGTCTCCTTAAAAA CCAAAAAGGAATGCATTAATCCACATTTCCCTTCTTCAGAGGTGTAGTGTCCTGGCTCTTGGCAAGGAAT TATGTATTTAGGTCAGTCCCCAGAAATGCAGCTGCTCATACAGCTGAGAGAAGGCTGTTATTGAGTTCCT TTACTTACTTTTTATACTACACTGATGCTGCTTGATAGAAGTCTGTGGCTTTGTCAGATATGTCACCCCA GTAAATGCTTTGTAGATCTGATTAAAATGAAAAGCTCACTTGAGAAACACTGCAGAGTTATGTAATGATC TTGTTGTGTGTGAAAGTCAAAGGCATGTCAGTTTATTACATTTGCAACATAAAAGTACTTAATTAAAATA GATATTTAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG
Notes:	The probe template was PCR amplified from IMAGE:3487943 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487943 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19474 same embryo
	EMAGE:19472 same embryo
	EMAGE:19473 same embryo
	EMAGE:19471 same embryo
	EurExpress:euxassay_004869 same experiment
	MGI:4827969 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source