EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19472

C1d C1D nuclear receptor co-repressor ( MGI:1927354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006080. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006080_01	euxassay_006080_02	euxassay_006080_03	euxassay_006080_04

euxassay_006080_05	euxassay_006080_06	euxassay_006080_07	euxassay_006080_08	euxassay_006080_09

euxassay_006080_10	euxassay_006080_11	euxassay_006080_12	euxassay_006080_13	euxassay_006080_14

euxassay_006080_15	euxassay_006080_16	euxassay_006080_17	euxassay_006080_18	euxassay_006080_19

euxassay_006080_20	euxassay_006080_21	euxassay_006080_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19472_wholemount_strong.wlz

19472_wholemount_moderate.wlz

19472_wholemount_weak.wlz

19472_wholemount_possible.wlz

19472_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19472_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6542
Entity Detected:	C1d, C1D nuclear receptor co-repressor ( MGI:1927354)
Sequence:	sense strand is shown >T6542 GACCTATTTCCCGGAGACAGGCGTCCACGGTATTGAGTTGGTCACAATGGCAGGTGAAGAAATGAATGAA GATTATCCCGTAGAAATTCACGAGTCTTTAACAGCCCTGGAGAGCTCCCTGGGTGCTGTGGATGACATGC TGAAGACCATGATGGCTGTTTCTAGAAATGAGTTGTTGCAGAAGTTGGACCCATTGGAACAAGCAAAGGT GGATTTAGTTTCTGCATACACCTTAAATTCAATGTTTTGGGTTTATTTGGCAACTCAAGGAGTTAATCCC AAAGAGCATCCAGTGAAGCAGGAACTGGAAAGAATCAGAGTCTACATGAACAGAGTTAAAGAAATAACAG ACAAGAAGAAGGCTGCCAAGCTGGACAGAGGTGCTGCTTCGAGATTTGTCAAGAACGCACTCTGGGAACC CAAAGCAAAAAGCACACCAAAAGTGGCTAATAAAGGGAAAAGCAAACACTAATCTTTTGGTTTTGATGTA CATGTTTTCAAAAAGTACATCCTTTTTAATCAGTTTACAATGTAGTTATGTGACCATGTGGTGTTTAAAT GGATTCCTTTTGGAATTCATGTATAAATTTACACATTACATTTGTGATACTGAATCTTTTTTTTGCTGAG AAAGATTAAGTTGTCTTTGTTGATTTTCATATAAAGCATCATGATGTGTTTAATATTGTAAGATATTCTA TAAGCAGTTGTGAAATCCAAATGTTCTCTGTAAACATTTGTAGTGTTTGAAATGAACAATGATATTATGA AGTGTGCTATCTGTAGACTGGAGGTGTAAGGACATTTGTTTTCAGTAATGATGAGAAATACAGTGACTTA AATACCCACTCTGTTTTCTGTTCAGTTAGTTCAACATGTTTCTGTGATTTTTTTTTTTTGGAGTAATTGC TGTCTTGATATTCAAAGTACAAAATTGAAACCTTAAGGCTGTACTTTAATTCTTCATGTTCCATTTAAAA TAAAATGTTCTCATTAACTCTGATGGAAAACACGGTTGTATGTCCTGACTGGCTTTGGCTTGATTCCCTT TTAATAAGTATGGTAGCTGGGTAGCTCACATGCTTCTTGCTGTCCACTGCGTGAGTGAACAAGCCCTCCA GATGAGGCAGTGTGAGCAGCTCCACCCGGTAGAGAAGGCTGATGAGTCATGGCGGCTCTCTGTTCTCTTG GTCTCACTCTTAGAATCTTTCTGACTAAGTATTTCCTAAGCCATGTGGATGTTTTGAAATATCTTCTTTA TTAAATACTGCCCCTTAGGCACAGGAGGAATCAAATTATATTTACATTCTGATTTATTTTTCCAGGGTGT TCGTTATGATGAATTCTACTTATTAATGTCTGAGCTCATATAACCTTGCTTCTCTAGGCGCACACAGTCA TGTGTAGAAGTGCCAAGGATAGCTAAGGTTTTTTTTGTTGTTGTTCTTGAGAATTAGAGTTGTAGAAATT GTCTGTGACTAACTTTTAATATATGAATTTCTTGATTAGGTAGCGGATAGTGGAACTGGTCTGAAGTTAT GATGGACTTTGTAAGGCATGCTAAAATCTGATTCATAAACAGTAAGTCAATAGATGCAGAAAGTCTCAGC TCTGATATCAGATACTTTGTGATGTGAAAGAACATTTATGAGCATTTGTAGTTATTTCACTCTGGGCCAT TTTAATTCTCTGATAATAGTGAAAGTAACTTCATTAGAGAAATAAGTGCTTTATGAAGATAACTGTAGAA TGCATTTTTAATGTTATTCGCTGCAAAGAAACTTGGGTCTTGTATTTCTTTTTAAATTTCATAAAGAAAA GAGCTTTTAATGGAAATTTTCACAATTGCAGAAAATAATTAACCAAGGTATTTGTGAACACCCAGCCTAT GTCCCACGGAATTGGATATCTTTAACGAGTTCTCTCAGCATGCGTTTGTAGAAATAAAATATTTTAAGTC TGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591682 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591682 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19474 same embryo
	EMAGE:19470 same embryo
	EMAGE:19473 same embryo
	EMAGE:19471 same embryo
	EurExpress:euxassay_006080 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source