EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19471

Psmd8 proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 ( MGI:1888669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006093. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006093_01	euxassay_006093_02	euxassay_006093_03	euxassay_006093_04

euxassay_006093_05	euxassay_006093_06	euxassay_006093_07	euxassay_006093_08	euxassay_006093_09

euxassay_006093_10	euxassay_006093_11	euxassay_006093_12	euxassay_006093_13	euxassay_006093_14

euxassay_006093_15	euxassay_006093_16	euxassay_006093_17	euxassay_006093_18	euxassay_006093_19

euxassay_006093_20	euxassay_006093_21	euxassay_006093_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19471_wholemount_strong.wlz

19471_wholemount_moderate.wlz

19471_wholemount_weak.wlz

19471_wholemount_possible.wlz

19471_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19471_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vibrissa

moderate

regional

moderate expression: see section 03 04 05 06 19 20 21

nose

strong

regional

strong expression: see section 11

naris

strong

regional

strong expression: see section 09 10 13 moderate expression: see section 14

urethra of male

weak

regional

weak expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6543
Entity Detected:	Psmd8, proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 ( MGI:1888669)
Sequence:	sense strand is shown >T6543 GCCGTAAGTCGGGTCGGCGGCTTGCCGCATCACGGAAGATGGCGGCCACAGCGGCTACGGTGAACGGGTC GACCACCGTCTCGAGCTCCGGACCCGCGGCCACCTCGGGCGGGATCCTGCAGGCAGCGGCCGGCATGTAC GAGCAACTCAAGGACGAGTGGAACCGGAAGAGCCCGAACCTTAGTAAATGTGGAGAAGAACTGGGCCGCC TGAAGTTGGTTCTGCTTGAGCTCAATTTCCTGCCAACCACGGGGACCAAACTGACTAAACAGCAACTCAT TCTGGCCCGTGACATTCTGGAGATCGGGGCCCAGTGGAGCATCCTGTGCAAAGACATTCCCTCCTTCGAG CGCTACATGGCCCAACTCAAATGCTACTACTTTGATTACAAAGAACAGCTTCCTGAGTCAGCCTATATGC ACCAGCTCCTGGGCCTCAATCTCCTCTTCCTGCTATCTCAGAACCGAGTGGCTGAGTTCCACACAGAATT AGAACGATTACCTGCCAAGGACATCCAGACCAATGTCTACATCAAACACCCTGTTTCCCTCGAGCAATAC CTCATGGAGGGTAGCTACAACAAGGTATTCCTGGCTAAGGGGAACATCCCTGCCGAAAGCTACACCTTCT TCATTGACATCCTGCTGGATACCATCAGGGATGAAATCGCAGGATGCATTGAGAAGGCCTATGAGAAAAT CCTTTTTGCTGAGGCTACCCGGATCCTCTTCTTCAGCACACCCAAAAAGATGACAGACTATGCCAAGAAG CGAGGTTGGGTGCTGGGCCCTAACAACTACTACAGCTTTGCCAGCCAGCAGCAGAAGCCAGAAGACAGCA CCATCCCCTCTACTGAACTGGCCAAACAAGTCATCGAGTATGCCCGGCAGCTGGAGATGATTGTCTGAGG CTTGACCAGGGCGGTGGATATGGCTAGTCCAACAAACCCAGTATAGGCTGGCCTTCAATAAAGGCAGATT AACCTCTAATGGGCCCGAGTCTCCCCAGTGTGATGGTGGAGATAAATTGCTCCTGGAGATTTGGGAGTTT GCGAAGCGTTTCCCAGTTGTCATTGCTCTGGGTTTTAAGTATAGATGCCCGGTGGCCCACAGGAGAGCAC AGGCCCAGACTGAGGGACTCTTCTAGCCAGCTGTGGATGCATTTTGGTGTTGGGCCAGGGACTAGATTTT TTTTTCCCCAGTGAAGCACCGTGTGTCTGTTTTAAAAAAATGTTGGCCGTGGATTAAATAATTTTTAAAA TGAGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591796 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591796 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19474 same embryo
	EMAGE:19470 same embryo
	EMAGE:19472 same embryo
	EMAGE:19473 same embryo
	EurExpress:euxassay_006093 same experiment
	MGI:4827489 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source