EMAGE mouse gene expression spatial database

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EMAGE:19512

Telo2 TEL2, telomere maintenance 2, homolog (S. cerevisiae) ( MGI:1918968)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004939. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004939_01	euxassay_004939_02	euxassay_004939_03	euxassay_004939_04

euxassay_004939_05	euxassay_004939_06	euxassay_004939_07	euxassay_004939_08	euxassay_004939_09

euxassay_004939_10	euxassay_004939_11	euxassay_004939_12	euxassay_004939_13	euxassay_004939_14

euxassay_004939_15	euxassay_004939_16	euxassay_004939_17	euxassay_004939_18	euxassay_004939_19

euxassay_004939_20	euxassay_004939_21	euxassay_004939_22	euxassay_004939_23	euxassay_004939_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19512_wholemount_strong.wlz

19512_wholemount_moderate.wlz

19512_wholemount_weak.wlz

19512_wholemount_possible.wlz

19512_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19512_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6946
Entity Detected:	Telo2, TEL2, telomere maintenance 2, homolog (S. cerevisiae) ( MGI:1918968)
Sequence:	sense strand is shown >T6946 GTAGGAGTGGTTGGTCCAGTCGGGGCTGCAGTGTCCTGCCTTGCAGCATGGCCAGTTCCCGTACGAGCCG AAGGCCTCGTGTTTAGGGGGCTGGGTGCTAACCTGGAGCTGCAGACGGAGGAGGAGTTTTAGTTATTGCA CCCTGGCAAGCCACTCACTCATCCAACTATCAGTGGGTTTGACAGAGGAAGCAGCCACAGTGCACCGGCG CAGTCCGGGACTGAAGCTCTGTCAGATCATCGGGCAGGCGGGAAACCCGGAAGAAGGACGGGATAGCGTG AGTGAGATTGTGAGTGAAGCGCCTGGAAGCGCAGAACCCTGTTCTGCAGGATGGACCCAGCCCTGTCTGC TGTCCGGCTCACCGTCCAGGAAGCCATTCACACCCTTTCATCCTCAGAGGATGCTGGCCATATCCTTTCT ACCCTGGGGACTCTGAAACGATACCTCGGTGGAACCGAGGATCCAGTTCTTCCTGAGGAGAAGGAGGAGT TTGCCACTGTCCACTTCTCAGCCGTCCTTAGATGTCTGGTCAGCAAGCTGAGCCCAGGTTGGCTGGAGCT GTCCCCCGGTGGCCAGCTGGAGCGGCTTTGGGAGAGCTTCTTCCTGGATGGCCCTCCAGACCAAGCCTTC CTGGTGCTGATGGAAGCCATTGAGAGCACTGCTGGCCCTAGCTTCCGTCTGATGAAGATGGCTCAGCTGC TGGACACATTTCTGAGCACGGGCAGAGTGGCTGCTCTAATGGAGGAGCAGTGTCGGCCACAGACAAAGCC CAGCTTTCCCCTGTTCCAGGAGACGCTGCTCAGCAAGGTTGTGGGCTTACCTGATCTCCTGGGCAACTGT CTCCAGCGGGACAACCTGACCCAGTTCTTCCCCCAGAACTACTTCCCTCTGCTTGGCCAGGAGGTTGTGC AGGCACTAAAGGCAGTTGTGAACTTTCTCCAAGGTGGCCTAGACTGCTCTGTCTCCTTCGTATCTCGAGT TCTTGGGAAGGTCTGCATCCAGGGCCGAAAGAGAGAGATCCTGAGTGTGCTGGTGCCCCAGCTGACAGTG CTCACCCAGGACAGCTGTCTGTGGCAGCGGGTGTGCTGGCGCCTGGTGGAGCAGGTGCCTGACCGGGCAG TGGAGGCTGTGCTGACAGGACTTGTAGAGGCTGCTCCCAGGCCTGAAGTCCTGTCACGACTACTAGGGAA CCTGGTGGTGAAGAATAAGAAAGCGCGGTTTGTGGTGACCCGCAAGCTCCTGCTCCTGCAGTACCAGCAC ACGACACCCATGGTGCAGAGCCTGTTGGGGTACTTGGCTCTAGACAGTCAGCGGCGGCCACTCCTCATAC AGGTGCTTAAGGAGCTGCTGGAGACCTGGGGCTGCAGCAGTGCTGTCCGACACACACCCCTGGAGCAGCA GTGTTACATCAGCAAGGCCATCCTTGTCTGCCTGGCACACCTCGGGGAGCCGGAGCTGCAGGACATCCGG GATGAATTGCTGGCCAGCATGATGGCAGGCGTGAAGTGCCGCCTGGATAGCAGCCTGCCCCCTGTGCGCC GCTTGGGCATGATTGTGGCCGAGGTCATCAGCTCCAGGATCCACCCTGAGGGGCCTCTCCTGAAATTCCA ATATGAAGATGACGAGATGAGCCGTGAGTTGCTGGCCTTGGCTACCCCAGAGCCTGCGGGTGACTGCTCC TCGGTGTCACGAGGCCCATCCCCAGCTCCTGCTGACACAGAGCCTCCTGTAGAGATGCCAGAAAAGGCCG TGGAGAGTGATGTCCCCCCAACTCAGCCGCAGGGCTCTGACTCGGAGCTGGACAGTGACGATGAGTTCAT CCCCTATGACATGTCAGGGGACAGAGAGCTGAAGAACAGCAAGGAGCCCCTGTATATCCGAGACTGCGTG GAAGCTTTGACTACATCTGAGGACATGGAACGCTGGGAGGCGTCCCTAAAGGGGCTTGAGGGCCTGGTAT ATAGGAGCCCCGCGGCCACTCGGGAGGTGAGTGTGGAGCTGGCCAAAGTGCTACTGCACCTGGAAGAGAA GACCTGTGTAGCAGAGTTTGAGCAGCTGCGCCAGAGCGCTCTGGTAGCTGTTACAGTCACAGACCCGGAG CAGGTAGCCAAGTATCTGACCTCACAGTTCTATGGCCTAAACTATAGCCTCCGGCAGCGCATGGACATCC TGGACGTCCTTGTTCTGGCTGCCCAGGCACTGTCTCGGCCAAAGAGCCTGCAGAGACGTTCTCAGCACGG TTCCCTCGTTCCTGGCACCATGTGTTCACCAGCACTAGCCGTTTCTCAGACTGGCAATGTCGCTGCTCCT GACTGGCAGATGGTTGTGGAGGAGCGGATCAGAAGCAAGACCCGGAGGTTCTCCAAGGGCTGTCCTCAGC GGGAGCTGTCAGGCGTCCCCAATGAATTCAGCTCTGTGGCTGGCTACTTCTTCTTCCCCCTCCTTCAGCA CTTTGACAGGCCTCTGGTGACCTTCGACCTTTTAGGAGATGATCAGTTGGTACTTGGAAGACTGACCCAC ACCTTAGCGTCCCTGATGTACCTGGCTGTTAACACCACAGTGGCTGTGCCTATGGGTAAGGCCCTGCTGG AGTTTGTATGGGCCCTTCGCTTCCACGTTGACATTTACGTGCGCCGGGGCTTGCTGTCTGCTGTGTCCTC TGTCCTCCTCAGTGTACCCACAGAGCGGCTGCTGGGGGACCTGCCAGATGAGCTACTGGAAGCCAGATCC TGGTTGGCAGATGTGGCTGAGAAGGATGTGGACGAGGACTGTAGGGAGCTGGCAGTAAGGGCTCTGCTGC TTCTGGAGAGACTCAAAGACAAGCTCCTGTCCAGTTCCTCTCCACAGCCCTGAGGAGTCTCCACAGGGGC CCGAATGGAGCCTGGGCCATCAGCACTGGAAGAAGGGAAGACCTCTGACAAGCAGAGTCCACAGGCCTGG GCTCTATGTGGAGAGGCTGCTGGTCCCTGTGTGCATGAAGTAGGCTCCTATCAATCTGACCCCTGCTGTT AACTTATTCCTTGGCCAGGCTAAGCGTTCAGCTAAAAATACTGGGGGGTCTGTGCCAAGGGACACAACAG CCTTTGACAGAATTGGGGCTGGGGTGTGGGGCCCGAGACCTGCCTCCTGAGAGCAGAACTTTGGGCTCTA TGGGCAGGGCCTCAAGAGTAAAGACAGGAGACAGCTACTCAGTCTTTACTATACTCGGATGGGGATGCTA GCTGCTGGCTCCCCATGGGCTGTGCAACACTTGTGTTCCTGTGAGAATAAACAGGCTACAGATGAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3984911 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3984911 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19516 same embryo
	EMAGE:19515 same embryo
	EMAGE:19513 same embryo
	EMAGE:19514 same embryo
	EMAGE:19511 same embryo
	EurExpress:euxassay_004939 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source