EMAGE mouse gene expression spatial database

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EMAGE:19515

Eno4 enolase 4 ( MGI:2441717)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004944. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004944_01	euxassay_004944_02	euxassay_004944_03	euxassay_004944_04

euxassay_004944_05	euxassay_004944_06	euxassay_004944_07	euxassay_004944_08	euxassay_004944_09

euxassay_004944_10	euxassay_004944_11	euxassay_004944_12	euxassay_004944_13	euxassay_004944_14

euxassay_004944_15	euxassay_004944_16	euxassay_004944_17	euxassay_004944_18	euxassay_004944_19

euxassay_004944_20	euxassay_004944_21	euxassay_004944_22	euxassay_004944_23	euxassay_004944_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19515_wholemount_strong.wlz

19515_wholemount_moderate.wlz

19515_wholemount_weak.wlz

19515_wholemount_possible.wlz

19515_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19515_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6945
Entity Detected:	Eno4, enolase 4 ( MGI:2441717)
Sequence:	sense strand is shown >T6945 AAAGAATTGGAAAAGAGCCAGGAGGAGCTGGTTCCCGCACCTCCACCAGTAACGCTGCCGCCGCCGCCGC CGCCGCCTCCCCCACCTCCCTCCAAAAAGAAAGGGCAGAAGGCAGGACGGAGGGATACGCTTTTGGAGAA ACCTGTTTCACCCCCGGAGCCCCCTGAACCAGTCCTCCATGGTAGCATGGCCATAGGCGCTGTGTCCCTG GCAGTGGCCAAAGCCAGCGCAACCTTGGCCAGCGATCCTCTCTACTTAACCCTGGCGTCACTGAAGCATG ATCAGGAGCAGCCGTCAACATTCTCTATGCCTTTGCTGATGGGATCTGTACTGAGCTGTGGGAAGTCGTC ACCTGGGAAGCTACATTTAATGAAAGAAGTGATTTGTATTCCCAGTCCTGGATTAACAGCCAAACAAAGT GTCGAGTTGCTTCTGGAAATTCAGAAACAAGTTAACAGAGCGATGGAGACGCTTCCACCTCCAAAACAAG AGACCAAAAAAGGGCATAATGGGAGCAAAAGAGCTCAGCCACCGATCACTGGCAAGGTATCTCACCTTGG CTGTTTAACCATCAACTACGACGCCATAGAGCAGCCACTGCTGCTGCTTCAGGGGATCTGCTCAAACCTG GGGTTGGAACTGGGAGTGAACTTCCATCTAGCCATCAACTGTGCTGGGCATGAGCTGATGGACTACAGTA AAGGGAAGTACGAAGTGATGGTGGGCACCCACAAAAGCGCAGCTGAGATGGTGGAGCTCTACGTGGACCT GATCAACAAGTATCCTTCCATAATCGCCTTAATTGATCCTTTCAGGAAGGAGGACGCCGAGCAGTGGGAC AGCCTCTATGCTGCTCTGGCTTCCCGGTGCTACCTAATTGCAGGCGCCGCTTCCGGGAGTGTCTCTAAAC TCCTAGAGTGCAGAAATATAAGCACCCTGAAATCCCACGGACTGATCATAAAGCACACAAACCAAACCAC AATGTCTGACTTGGTGGAAATAACCCATCTTATCAACGGTAAGAAGCTCCTGGCCGTCTTTGGAAGCACA GACTCGGAGTCCTCTGATGACAGCCTTGTCGATTTGGCTGTTGGATTCGGTGCCCGGTTTATCAAGTTGG GGGGTCTTTCTCGGGGTGAACGGATGACCAAATACAACCGCCTTCTTGCTATAGAGGAAGAACTCATCCA GAGGGGAGTATGGGGATGCTACTGCCACAATGGCTGAGGAAACTCTTGGGCTCCTGGACTCCATCTTCCC CACAGAGGTGATAGAGGAATCGGCTAAAACATGAGCCTCCCTCCCGGTTCTGGACTCCCAGGGCACAGCC ACTCCACCAGTGTGGGCCAGCTGGTGTGAAGTGCCTCCACGGTGTGGCTCCGCTCTGAACTCCACTTATA TCCGTAAATGAGTATAATCACCAACGTGTGCATGCTTTCGGTTTCACCGTCGGGTAAAATATATGCCTGG TGGGTTTGCCTGCAGCTCATGAGCTTTACTTTCGAGGCCACTTTGCCATGGGACACTAGCTCCACTGTCT CCTTCTGAAAGTCATGACCTAAGTTCATTAGAGGCTAATGTATTTCCGATGTCGGGTTCACGCCCAGTCA CGCACCCCTCATAAATCAGAGCGGCTCTAGCACCAAAAGACTGTTTCCCTGAGTAGCTCCCGGCACCACA CAGGAATCCTCTGATTTTGTTTCTCTTGGACCTCTGTGCTTGGACCACAGATGCTCTGCCTAGCCACCAA CATGCACCAAGCCTTGTCTGCAGAGATACTTCCATCTTAGCTTCCAACATTGATTACCTGTGCCTGTATT GATACTTCTATCTTAGCTTCTGACACTTGATTACCTGTGTCTGTGCCTTTCCTCATCCCTCTGGTGTGTT CATCATCCCTGTATTATTTTTTCCCTTAGGTCCCTTTTGATACATGAGTGGGATTAACTTAATAAAAACA CTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3984456 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3984456 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19516 same embryo
	EMAGE:19512 same embryo
	EMAGE:19513 same embryo
	EMAGE:19514 same embryo
	EMAGE:19511 same embryo
	EurExpress:euxassay_004944 same experiment
	MGI:4824554 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source