EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19513

Zfp825 zinc finger protein 825 ( MGI:2385315)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004946. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004946_01	euxassay_004946_02	euxassay_004946_03	euxassay_004946_04

euxassay_004946_05	euxassay_004946_06	euxassay_004946_07	euxassay_004946_08	euxassay_004946_09

euxassay_004946_10	euxassay_004946_11	euxassay_004946_12	euxassay_004946_13	euxassay_004946_14

euxassay_004946_15	euxassay_004946_16	euxassay_004946_17	euxassay_004946_18	euxassay_004946_19

euxassay_004946_20	euxassay_004946_21	euxassay_004946_22	euxassay_004946_23	euxassay_004946_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19513_wholemount_strong.wlz

19513_wholemount_moderate.wlz

19513_wholemount_weak.wlz

19513_wholemount_possible.wlz

19513_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19513_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6948
Entity Detected:	Zfp825, zinc finger protein 825 ( MGI:2385315)
Sequence:	sense strand is shown >T6948 GTTTTTTGCTGTACAGGCTTGAATGTTTCTCCGTGTCTTCTTTGAGCGCTGCTATTGGGTGCTGTGAGGG GACGTCAAGTAAGCTCCGTTTTTGAAACATGGATGCAGTGACTTATGATGATGTGCGTGTGAACTTCACT GAGGAAGAGTGGGATTTGCTGGATCCTTCCCAGAAAAGTCTCTATGAAGAGGTGATGCTGGAGACCTACC AGAACATCACTGCTATAGGCTACAAGTGGGAAGACCATCATATTGAAGAATATTGTCAAAGTTCTAGAAG ACATGAAAGGCATGAAAGAATTCATATTGGAGAGAAACCCTATGAACGTAACCAATGTGGTAAAGCCTTT GCAAGTGACAATCATCTCCAAATACATAAAAGAACAAATACTGAAGAGAAACCTTATGAATGTAATCAAT GTGGTAAAGCCTTTTCACAACACAGTAGACTCCAATATCATAAAAGAAGTCATACTGGAGAGAAACCCTA TAAATGTAATCAGTGTGGTAAAGCCTATGGACATCACAGTAGTCTCCAGTATCATAAAAGAAGACATACT GGAGAGAAACCTTATCACTGTAATCAATGTGATAAAGCATTTTCAGAACACAGTAGTCTTGAATATCATA AAATAACACATCTTGGAGAGAAACCTTATGAGTGTGATCAATGTGGTAAAGCCTTTGCATGGCAGTCTAG TCTCAATATACATAAAAGAACACATTCTGGAGAGAAACCTTATGAGTGTAATGAATGTGGTAAAGCCTTT ACACATCATGCTAGTCTCCAAAGTCATAAAAGAATACATACTGGAGAGAAACCGTATCAGTGTGATCAGT GTGGTAAAGCATTTGCACATCAAACGAGTCTCCAATGCCATAAAAGAACACATACTGGAGAGAAACCTTA TGAATGCAATTAATGTGGTAAAGCCTTTGCAAGTCACACTCATCTCCAAGTACATGAAAGAACACATTTT AGAAAAAAACCTTAGAATGCAATCAATGTTGTAAAGACTTTGGATGTAAGAATAATCTCCAAAGACATAA AGGAACATATACTGGACAGAAACCTTATGAATGTAATCAAAGTGATAAAGCCTTTGCAACTCACAGTCAT CTACAATGACATAAAAACACAGAGGAGAGAAACCTGGGTATTGTTTACTCTGAAGCTCCACCAAGTGCAA AACAGACCCTCCCATTCCCTCCCTGGAAGACAGTTTCTTCCTGACTGCCTTTGGATCAAAATGCAGAACT CTCAGCTCCTTCTCCAGCATCATGTCTGCCTGCATGCTGCTGCTGTGCTTCCTGCTATGATGAAAATGGA CTAAACCTCTGAAACTGTAAGCCAGCCCCAATTAAAGGTTTGCTTTTATGAGAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3985027 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3985027 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19516 same embryo
	EMAGE:19512 same embryo
	EMAGE:19515 same embryo
	EMAGE:19514 same embryo
	EMAGE:19511 same embryo
	EurExpress:euxassay_004946 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source